Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A24 F30 I1 R1	18	33.7	1149868	98.9%	1137219	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,505,167	C→A	G370G (GGC→GGA)	yjhB →	putative sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,505,167	0	C	A	100.0%	118.0 / NA	34	G370G (GGC→GGA)	yjhB	putative sialic acid transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (22/12); total (22/12)

TTGGTTCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGC  >  NC_000913/4505033‑4505301

ttggttCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAgg                                                                                                                                   >  2:81555/1‑140 (MQ=255)

ttggttCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAgg                                                                                                                                   <  1:91053/140‑1 (MQ=255)

   gttCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGgtgt                                                                                                                                >  2:180139/1‑140 (MQ=255)

                      aTTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACg                                                                                                             >  2:128197/1‑140 (MQ=255)

                       ttACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACgg                                                                                                            <  1:239777/140‑1 (MQ=255)

                                  caaAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTctct                                                                                                 >  2:126524/1‑140 (MQ=255)

                                               aTTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAAttt                                                                                    <  2:515919/140‑1 (MQ=255)

                                                     gACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAg                                                                              <  1:126524/140‑1 (MQ=255)

                                                      aCCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGt                                                                             <  1:488020/140‑1 (MQ=255)

                                                        cGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTg                                                                           >  2:329144/1‑140 (MQ=255)

                                                         ggTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTgg                                                                          >  1:559452/1‑140 (MQ=255)

                                                          gTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGt                                                                         >  2:6276/1‑140 (MQ=255)

                                                                          aGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTa                                                         >  2:254568/1‑140 (MQ=255)

                                                                          aGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTa                                                         >  2:481958/1‑140 (MQ=255)

                                                                           ggggCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTaa                                                        <  2:544111/140‑1 (MQ=255)

                                                                                  cTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTaa                                                        >  1:218017/1‑133 (MQ=255)

                                                                                  cTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTaa                                                        <  2:218017/133‑1 (MQ=255)

                                                                                                   ccTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTaa                                <  1:6276/140‑1 (MQ=255)

                                                                                                            gCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGa                       <  1:254568/140‑1 (MQ=255)

                                                                                                             cTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAg                      >  2:259/1‑140 (MQ=255)

                                                                                                                 atacatTTCAGGATATTATGGATTGGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGc                                   >  2:19768/1‑123 (MQ=255)

                                                                                                                 atacatTTCAGGATATTATGGATTGGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGc                                   <  1:19768/123‑1 (MQ=255)

                                                                                                                 atacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCtt                  >  2:284366/1‑140 (MQ=255)

                                                                                                                 atacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCtt                  >  2:46535/1‑140 (MQ=255)

                                                                                                                 atacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCtt                  >  1:425883/1‑140 (MQ=255)

                                                                                                                 atacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCtt                  >  2:532545/1‑140 (MQ=255)

                                                                                                                  tacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTAGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCttt                 <  1:81555/140‑1 (MQ=255)

                                                                                                                       ttCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGat            <  1:128197/140‑1 (MQ=255)

                                                                                                                          aGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGattaa         >  1:390088/1‑140 (MQ=255)

                                                                                                                           ggATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTAATAAACTATCCGTGGCTAAATGATTGGAGGCTTAATGattaat        >  2:103136/1‑140 (MQ=255)

                                                                                                                                 tatGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGc  >  2:388170/1‑140 (MQ=255)

                                                                                                                                 tatGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGc  >  2:444934/1‑140 (MQ=255)

                                                                                                                                 tatGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGc  >  2:455452/1‑140 (MQ=255)

                                                                                                                                 tatGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGc  >  1:400753/1‑140 (MQ=255)

TTGGTTCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGC  >  NC_000913/4505033‑4505301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A