Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 145 97.8 3401598 99.2% 3374385 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,638,743 A→G D189G (GAC→GGC)  creD → putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,638,7430AG98.5% 235.0 / ‑4.4 67D189G (GAC→GGC) creDputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (40/26);  total (40/27)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

TGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGAT  >  NC_000913/4638608‑4638879
                                                                                                                                       |                                                                                                                                        
tgATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTgg                                                                                                                                      >  2:1273123/1‑140 (MQ=255)
  aTTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCg                                                                                                                                    <  1:662607/140‑1 (MQ=255)
  aTTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCg                                                                                                                                    <  2:119615/140‑1 (MQ=255)
            gggATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAAc                                                                                                                          >  1:190397/1‑140 (MQ=255)
             ggATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAAcc                                                                                                                         <  1:240277/140‑1 (MQ=255)
             ggATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAAcc                                                                                                                         <  1:200575/140‑1 (MQ=255)
                  cgcgTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAg                                                                                                                    >  2:935896/1‑140 (MQ=255)
                            tggtgtggtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATTCCTTTGCCTGTAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATgg                                                                                                          <  2:1462494/140‑1 (MQ=255)
                              gtgtggtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGcc                                                                                                        <  1:1273123/140‑1 (MQ=255)
                              gtgtggtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGcc                                                                                                        <  2:981852/140‑1 (MQ=255)
                                 tggtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTg                                                                                                     >  1:1215284/1‑140 (MQ=255)
                                   gtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGaa                                                                                                   >  1:868756/1‑140 (MQ=255)
                                   gtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGaa                                                                                                   >  2:1539594/1‑140 (MQ=255)
                                   gtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGaa                                                                                                   >  1:61252/1‑140 (MQ=255)
                                   gtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGaa                                                                                                   >  1:599671/1‑140 (MQ=255)
                                   gtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGaa                                                                                                   >  2:853690/1‑140 (MQ=255)
                                   gtgAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGaa                                                                                                   >  2:132902/1‑140 (MQ=255)
                                     gAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAAtt                                                                                                 <  1:1539594/140‑1 (MQ=255)
                                       aaGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTa                                                                                               <  2:61252/140‑1 (MQ=255)
                                        aGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAAt                                                                                                  <  1:1394368/136‑1 (MQ=255)
                                        aGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAAt                                                                                                  >  2:1394368/1‑136 (MQ=255)
                                         gcgcCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAg                                                                                             <  1:73215/140‑1 (MQ=255)
                                                aaGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTAcc                                                                                      <  1:375944/140‑1 (MQ=255)
                                                          aacggCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCttt                                                                            >  2:1320714/1‑140 (MQ=255)
                                                          aacggCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCttt                                                                            <  1:132902/140‑1 (MQ=255)
                                                          aacggCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCttt                                                                            >  2:1422934/1‑140 (MQ=255)
                                                          aacggCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCttt                                                                            <  2:190397/140‑1 (MQ=255)
                                                           acggCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTc                                                                           >  2:362082/1‑140 (MQ=255)
                                                               cgcTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATACCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCtgtg                                                                       >  1:1140572/1‑140 (MQ=255)
                                                                gcTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTgtgg                                                                      <  1:853690/140‑1 (MQ=255)
                                                                    aCCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCATAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGcc                                                                  >  1:356107/1‑140 (MQ=255)
                                                                      cATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTg                                                                <  1:959913/140‑1 (MQ=255)
                                                                        ttGAACCCGGCGCCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCAGCGATCTTTCTGTGGTGCCTggc                                                              <  2:883535/140‑1 (MQ=255)
                                                                        ttGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTggc                                                              <  1:1128892/140‑1 (MQ=255)
                                                                         tGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTggcg                                                             >  2:438570/1‑140 (MQ=255)
                                                                             cccGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTggcgggcg                                                         <  2:868756/140‑1 (MQ=255)
                                                                                ggCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTaa                                                      >  1:184486/1‑140 (MQ=255)
                                                                                ggCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTaa                                                      >  2:418493/1‑140 (MQ=255)
                                                                                  cgcaggggTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTAACTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATa                                                    >  2:250921/5‑140 (MQ=255)
                                                                                  cACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATa                                                    <  2:1291868/140‑1 (MQ=255)
                                                                                           agagCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGa                                           >  1:671509/1‑140 (MQ=255)
                                                                                             agCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGAcc                                         <  1:438570/140‑1 (MQ=255)
                                                                                              gCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCt                                        <  1:418493/140‑1 (MQ=255)
                                                                                               cAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCtt                                       >  2:1251795/1‑140 (MQ=255)
                                                                                                  ggCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAAc                                    >  1:63812/1‑140 (MQ=255)
                                                                                                  ggCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCAGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAAc                                    >  2:972248/1‑140 (MQ=255)
                                                                                                      ggCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGGGTAATAGCGAAATGACCTTAAACAGc                                >  2:1685754/1‑140 (MQ=255)
                                                                                                       gCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCa                               >  2:194331/1‑140 (MQ=255)
                                                                                                       gCAGGGCGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCa                               >  1:1512552/1‑140 (MQ=255)
                                                                                                             cGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGc                         >  1:550954/1‑140 (MQ=255)
                                                                                                             cGTGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGc                         >  1:1649453/1‑140 (MQ=255)
                                                                                                               tGCATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCg                       <  1:1290504/140‑1 (MQ=255)
                                                                                                                 cATATCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCa                     <  1:1422934/140‑1 (MQ=255)
                                                                                                                     tCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATccc                 >  2:144512/1‑140 (MQ=255)
                                                                                                                     tCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATccc                 >  2:1089009/1‑140 (MQ=255)
                                                                                                                     tCCCTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATccc                 >  2:106015/1‑140 (MQ=255)
                                                                                                                      cccTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCCTcccg                >  1:65042/1‑139 (MQ=255)
                                                                                                                      cccTTTACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCa                >  1:742456/1‑140 (MQ=255)
                                                                                                                          ttACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGttt            >  1:263903/1‑140 (MQ=255)
                                                                                                                          ttACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGttt            >  2:343587/1‑140 (MQ=255)
                                                                                                                           tACCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGtttt           >  2:1647032/1‑140 (MQ=255)
                                                                                                                            aCCTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGttttt          <  2:1215284/140‑1 (MQ=255)
                                                                                                                             ccTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGtttttt         >  1:1300212/1‑140 (MQ=255)
                                                                                                                              cTGAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTa        >  1:1500551/1‑140 (MQ=255)
                                                                                                                                gAAGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAgg      <  1:1089009/140‑1 (MQ=255)
                                                                                                                                 aaGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGt     <  1:194331/140‑1 (MQ=255)
                                                                                                                                 aaGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGt     >  2:1282953/1‑140 (MQ=255)
                                                                                                                                 aaGGGGGCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGt     >  1:675826/1‑140 (MQ=255)
                                                                                                                                    ggggCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGAt  <  2:1500551/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
TGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGAT  >  NC_000913/4638608‑4638879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A