Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A31 F30 I1 R1 28 44.1 1499574 99.3% 1489076 139.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,085,456 G→T Q123K (CAA→AAA)  fdoG ← formate dehydrogenase O subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,085,4560GT100.0% 141.9 / NA 41Q123K (CAA→AAA) fdoGformate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/17);  total (24/17)

CGCGGAAGCACACAGCATCCCGGTGGAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGATA  >  NC_000913/4085319‑4085592
                                                                                                                                         |                                                                                                                                        
cgcgGAAGCACACAGCATCCCGGTGGAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTttct                                                                                                                                        >  2:80307/1‑140 (MQ=255)
cgcgGAAGCACACAGCATCCCGGTAGAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTttct                                                                                                                                        >  1:403525/1‑140 (MQ=255)
                  cccGGTGGAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTg                                                                                                                      >  1:537123/1‑140 (MQ=255)
                       tGGAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCa                                                                                                                 <  1:355526/140‑1 (MQ=255)
                         gagaGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACg                                                                                                               <  2:221957/140‑1 (MQ=255)
                            ggCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTa                                                                                                            >  1:459587/2‑140 (MQ=255)
                                      tAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAAc                                                                                                  >  2:733912/1‑140 (MQ=255)
                                           gTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAg                                                                                             <  2:424586/140‑1 (MQ=255)
                                              aCGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACg                                                                                          >  2:746495/1‑140 (MQ=255)
                                              aCGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACg                                                                                          >  1:671730/1‑140 (MQ=255)
                                               cGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACgg                                                                                         >  2:505822/1‑140 (MQ=255)
                                                    tCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTc                                                                                    >  2:244986/1‑140 (MQ=255)
                                                       gCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGa                                                                                 <  1:25455/140‑1 (MQ=255)
                                                       gCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGa                                                                                 <  2:473567/140‑1 (MQ=255)
                                                            ttGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGa                                                                            >  1:230223/1‑140 (MQ=255)
                                                                       aGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATccacc                                                                 <  1:195499/140‑1 (MQ=255)
                                                                         ttAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAg                                                               >  1:388677/1‑140 (MQ=255)
                                                                              aTCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAg                                                          >  2:607374/1‑140 (MQ=255)
                                                                              aTCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAg                                                          >  1:555929/1‑140 (MQ=255)
                                                                              aTCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAg                                                          >  2:12741/1‑140 (MQ=255)
                                                                              aTCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAg                                                          >  2:225392/1‑140 (MQ=255)
                                                                              aTCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAg                                                          >  2:231135/1‑140 (MQ=255)
                                                                              aTCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAg                                                          >  1:347862/1‑140 (MQ=255)
                                                                              aTCGCGGTCTTCTTTCATCAGTTTAGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAg                                                          >  1:475586/1‑140 (MQ=255)
                                                                                  cgGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCTGGCCAGCGcc                                                      >  2:510565/1‑140 (MQ=255)
                                                                                  cgGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGcc                                                      >  2:601400/1‑140 (MQ=255)
                                                                                   ggTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCt                                                     <  1:284810/140‑1 (MQ=255)
                                                                                        tcttTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCgg                                                <  1:496064/140‑1 (MQ=255)
                                                                                        tcttTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCgg                                                <  2:25138/140‑1 (MQ=255)
                                                                                               tcaGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCTGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAgg                                         >  2:343257/1‑139 (MQ=255)
                                                                                                  gTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCa                                      <  1:231135/140‑1 (MQ=255)
                                                                                                    ttGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCAcc                                    >  1:198294/1‑140 (MQ=255)
                                                                                                      ggCGATGCGATCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACcgc                                  <  2:658813/140‑1 (MQ=255)
                                                                                                        cgatgcgatCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCgg                                >  1:572334/1‑140 (MQ=255)
                                                                                                                tCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCgg                        <  2:403525/140‑1 (MQ=255)
                                                                                                                tCAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCgg                        <  2:572334/140‑1 (MQ=255)
                                                                                                                 cAAACGCCTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCggg                       <  1:80307/140‑1 (MQ=255)
                                                                                                                       ccTCTTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATcc                 >  2:648157/1‑140 (MQ=255)
                                                                                                                          ctTCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGa              >  1:266942/1‑140 (MQ=255)
                                                                                                                           ttCCCAACTGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGAt             <  1:554087/140‑1 (MQ=255)
                                                                                                                                   tGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGTTTTCGGGGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCg     <  2:293963/140‑1 (MQ=255)
                                                                                                                                   tGATTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCg     <  1:192553/140‑1 (MQ=255)
                                                                                                                                     aTTTTCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGat   >  1:365236/1‑140 (MQ=255)
                                                                                                                                      ttttCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGata  <  2:216400/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CGCGGAAGCACACAGCATCCCGGTGGAGAGCCAGCGGTTAACAGTCACGCCTTCGGCGTTTTGCGCAATGTAGTTAGCATCGCGGTCTTCTTTCATCAGTTTGGCGATGCGATCAAACGCCTCTTCCCAACTGATTTGCTGCCATTTATCAGAACCTGGCGCACGGTATTCCGGAAACTTCAGACGGCTTTCGGAGTGGATGAAATCCACCAGGCCAGCGCCTTTCGGACAAAGTGCACCGCGGTTGACCGGGTGATCCGGATCGCCTTCGATA  >  NC_000913/4085319‑4085592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A