Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	146	75.3	2562504	98.6%	2526628	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,955,096	T→C	E303G (GAA→GGA)	recB ←	exodeoxyribonuclease V subunit RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,955,096	0	T	C	94.2%	281.0 / 4.6	86	E303G (GAA→GGA)	recB	exodeoxyribonuclease V subunit RecB
Reads supporting (aligned to +/- strand): ref base T (3/2); new base C (39/42); total (42/44)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

TTCGCCACGGCGGCGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑TCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGT  >  NC_000913/2954960‑2955231

ttCGCCACGGCGGCGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑cccc                                                                                                                                      >  1:319983/1‑140 (MQ=255)

       cggcggCGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGc                                                                                                                               <  1:1041427/140‑1 (MQ=255)

       cggcggCGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGc                                                                                                                               <  1:441193/140‑1 (MQ=255)

             cgTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGt                                                                                                                         >  2:710619/1‑140 (MQ=255)

               tttttCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGc                                                                                                                       >  2:963155/1‑140 (MQ=255)

               tttttCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGc                                                                                                                       <  1:80054/140‑1 (MQ=255)

                ttttCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCg                                                                                                                      <  1:56392/140‑1 (MQ=255)

                ttttCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCg                                                                                                                      <  1:710619/140‑1 (MQ=255)

                ttttCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCg                                                                                                                      <  2:109116/140‑1 (MQ=255)

                ttttCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCg                                                                                                                      <  2:319983/140‑1 (MQ=255)

                ttttCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCg                                                                                                                      <  1:1176097/140‑1 (MQ=255)

                          cTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAg                                                                                                            >  2:800487/1‑140 (MQ=255)

                                tttCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCg                                                                                                                      >  2:743588/1‑124 (MQ=255)

                                tttCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCg                                                                                                                      <  1:743588/124‑1 (MQ=255)

                                    gcgGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTggg                                                                                                  <  1:963155/140‑1 (MQ=255)

                                     cgGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTTCCCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTggg                                                                                                  >  2:950032/1‑140 (MQ=255)

                                     cgGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGga                                                                                                 >  2:266005/1‑140 (MQ=255)

                                     cgGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGga                                                                                                 >  1:515295/1‑140 (MQ=255)

                                      ggATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGgag                                                                                                <  2:515295/140‑1 (MQ=255)

                                          ctcAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAAtt                                                                                            >  2:1014053/1‑140 (MQ=255)

                                          ctcAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAAtt                                                                                            >  1:33102/1‑140 (MQ=255)

                                                  aTGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGc                                                                                    >  2:596314/1‑140 (MQ=255)

                                                   tGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCg                                                                                   <  1:668984/140‑1 (MQ=255)

                                                   tGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCg                                                                                   >  1:1053179/1‑140 (MQ=255)

                                                   tGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCg                                                                                   >  1:1262239/1‑140 (MQ=255)

                                                          gTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCgg                                                                            <  2:592490/140‑1 (MQ=255)

                                                          gTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCgg                                                                            <  2:679973/140‑1 (MQ=255)

                                                          gTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCgg                                                                            <  1:266005/140‑1 (MQ=255)

                                                             aTCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCTACTCCgg                                                                            <  2:500981/137‑1 (MQ=255)

                                                             aTCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCTACTCCgg                                                                            >  1:500981/1‑137 (MQ=255)

                                                             aTCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCaa                                                                         <  1:235245/140‑1 (MQ=255)

                                                             aTCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCaa                                                                         >  2:1169580/1‑140 (MQ=255)

                                                                  cAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGAt                                                                    <  1:1169580/140‑1 (MQ=255)

                                                                    gATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGTATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCg                                                                                   >  1:240650/1‑123 (MQ=255)

                                                                    gATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGTATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCg                                                                                   <  2:240650/123‑1 (MQ=255)

                                                                    gATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATaa                                                                  >  2:688652/1‑140 (MQ=255)

                                                                    gATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATaa                                                                  >  2:682086/1‑140 (MQ=255)

                                                                    gATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATaa                                                                  >  1:544519/1‑140 (MQ=255)

                                                                    gATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATaa                                                                  <  2:1262239/140‑1 (MQ=255)

                                                                       cgcgGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTg                                                               >  1:733988/1‑140 (MQ=255)

                                                                       cgcgGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTg                                                               >  2:1164695/1‑140 (MQ=255)

                                                                       cgcgGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCACGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTg                                                               >  1:167012/1‑140 (MQ=255)

                                                                       cgcgGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGG‑T‑TCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTcc                                                                              >  2:337302/1‑124 (MQ=255)

                                                                       cgcgGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGG‑T‑TCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTcc                                                                              <  1:337302/124‑1 (MQ=255)

                                                                         cgGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGtt                                                             >  2:620512/1‑140 (MQ=255)

                                                                         cgGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGtt                                                             >  1:696139/1‑140 (MQ=255)

                                                                         cgGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGtt                                                             >  2:1263537/1‑140 (MQ=255)

                                                                         cgGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGG‑T‑TCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGttt                                                            >  1:1136199/1‑140 (MQ=255)

                                                                          ggATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGttt                                                            <  2:520225/140‑1 (MQ=255)

                                                                          ggATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGttt                                                            <  2:696139/140‑1 (MQ=255)

                                                                          ggATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGttt                                                            <  2:913682/140‑1 (MQ=255)

                                                                               gACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGtctc                                                       <  1:1263537/140‑1 (MQ=255)

                                                                               gACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGtctc                                                       <  1:619163/140‑1 (MQ=255)

                                                                                aCAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTctct                                                      >  2:588580/1‑140 (MQ=255)

                                                                                aCAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTctct                                                      >  2:650628/1‑140 (MQ=255)

                                                                                aCAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTctct                                                      >  1:51009/1‑140 (MQ=255)

                                                                                aCAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTctct                                                      >  2:75996/1‑140 (MQ=255)

                                                                                  aaTGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCtcttc                                                    <  2:51009/140‑1 (MQ=255)

                                                                                          tGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGccc                                            <  1:588580/140‑1 (MQ=255)

                                                                                          tGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGccc                                            <  1:650628/140‑1 (MQ=255)

                                                                                          tGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGccc                                            <  1:662418/140‑1 (MQ=255)

                                                                                          tGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGccc                                            <  1:75996/140‑1 (MQ=255)

                                                                                           gCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCa                                           >  2:396677/1‑140 (MQ=255)

                                                                                            cAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGG‑T‑TCCCCCCCGGCCTTCGGGCGGTCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAgg                                         >  2:979155/1‑140 (MQ=255)

                                                                                               gCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGgcg                                       <  1:688652/140‑1 (MQ=255)

                                                                                                cAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGgcgc                                      <  2:595498/140‑1 (MQ=255)

                                                                                                 aGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGGT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGCTTGTCTCTTCTTCTGCCCAGGCGCt                                     >  2:754783/1‑140 (MQ=255)

                                                                                                   ttGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGgcgc                                      <  2:618450/137‑1 (MQ=255)

                                                                                                   ttGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGgcgc                                      >  1:618450/1‑137 (MQ=255)

                                                                                                        cgatcgCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTg                              <  1:620512/140‑1 (MQ=255)

                                                                                                           tcgCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCg                           >  2:79378/1‑140 (MQ=255)

                                                                                                           tcgCCTCAAACAGTGGATGTCGCGGGG‑T‑TCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGa                          <  2:1136199/140‑1 (MQ=255)

                                                                                                            cgCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGa                          <  2:33102/140‑1 (MQ=255)

                                                                                                            cgCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGa                          <  2:733988/140‑1 (MQ=255)

                                                                                                             gCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGgcg                                       <  2:6417/126‑1 (MQ=255)

                                                                                                             gCCTCAAACAGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGgcg                                       >  1:6417/1‑126 (MQ=255)

                                                                                                                      aGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGc                <  1:165992/140‑1 (MQ=255)

                                                                                                                      aGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGc                >  2:23707/1‑140 (MQ=255)

                                                                                                                      aGTGGATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGc                <  1:23707/140‑1 (MQ=255)

                                                                                                                        tGGATGTCGCGGGGAT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCt              >  1:574994/1‑140 (MQ=255)

                                                                                                                         ggATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTg             >  2:443617/1‑140 (MQ=255)

                                                                                                                         ggATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTg             >  1:303453/1‑140 (MQ=255)

                                                                                                                          gATGTCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGa            <  2:1053179/140‑1 (MQ=255)

                                                                                                                              tCGCGGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGc        <  1:682086/140‑1 (MQ=255)

                                                                                                                               cgcgGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCt       <  1:800487/140‑1 (MQ=255)

                                                                                                                                gcgGGGTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTa      >  1:647829/1‑140 (MQ=255)

                                                                                                                                    ggTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGt  >  1:1216478/1‑140 (MQ=255)

                                                                                                                                    ggTT‑CCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGt  >  1:74732/1‑140 (MQ=255)

TTCGCCACGGCGGCGTTTTTCACGCGCTACTGTTTCGCGGATCTCAGCCAATGCGCGGGTGATCACCAGATCGCGGATCGACAATGGTTCTGCAAGCAGTTGATCGATCGCCTCAAACAGTGGATGTCGCGGGGTT‑TCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGT  >  NC_000913/2954960‑2955231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A