Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	146	75.3	2562504	98.6%	2526628	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,117,500	T→C	N538S (AAC→AGC)	yghJ ←	putative lipoprotein YghJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,117,500	0	T	C	97.4%	274.4 / ‑2.9	77	N538S (AAC→AGC)	yghJ	putative lipoprotein YghJ
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (40/35); total (41/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGAAGGCCGCGTTGGAGA  >  NC_000913/3117364‑3117635

cccGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGcttt                                                                                                                                      >  1:1125186/1‑140 (MQ=255)

    ttCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCgg                                                                                                                                  <  2:1155168/140‑1 (MQ=255)

    ttCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCgg                                                                                                                                  >  2:304305/1‑140 (MQ=255)

      ccAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCt                                                                                                                                <  2:150179/140‑1 (MQ=255)

       cAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTc                                                                                                                               <  1:750537/140‑1 (MQ=255)

             tAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAag                                                                                                                         >  2:1054133/1‑140 (MQ=255)

             tAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAag                                                                                                                         >  2:698377/1‑140 (MQ=255)

               aCCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAagag                                                                                                                       >  1:1105143/1‑140 (MQ=255)

               aCCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAagag                                                                                                                       >  1:993736/1‑140 (MQ=255)

                   ttCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAgg                                                                                                                   <  2:993736/140‑1 (MQ=255)

                   ttCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAgg                                                                                                                   <  2:1125186/140‑1 (MQ=255)

                   ttCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAgg                                                                                                                   >  2:215849/1‑140 (MQ=255)

                   ttCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAgg                                                                                                                   <  1:224374/140‑1 (MQ=255)

                    tCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGgc                                                                                                                  >  1:438695/1‑140 (MQ=255)

                     cGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGgcg                                                                                                                 <  1:1277336/140‑1 (MQ=255)

                     cGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGgcg                                                                                                                 >  2:952669/1‑140 (MQ=255)

                     cGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGgcg                                                                                                                 <  1:1253591/140‑1 (MQ=255)

                      gggCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGgcgc                                                                                                                <  2:438695/140‑1 (MQ=255)

                           aCGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCg                                                                                                           <  1:263653/140‑1 (MQ=255)

                           aCGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCg                                                                                                           <  2:1105143/140‑1 (MQ=255)

                           aCGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCg                                                                                                           <  1:422628/140‑1 (MQ=255)

                           aCGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCg                                                                                                           <  1:23512/140‑1 (MQ=255)

                            cGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGt                                                                                                          >  2:919780/1‑140 (MQ=255)

                                         tgtAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGc                                                                                             >  1:459027/1‑140 (MQ=255)

                                         tgtAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGc                                                                                             <  1:97532/140‑1 (MQ=255)

                                          gtAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCt                                                                                            <  1:708646/140‑1 (MQ=255)

                                                tgGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGtttt                                                                                      >  1:556653/1‑140 (MQ=255)

                                                  ggTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTa                                                                                    >  2:1044817/1‑140 (MQ=255)

                                                   gTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTAt                                                                                   >  1:1001941/1‑140 (MQ=255)

                                                     tACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATcc                                                                                 >  2:945838/1‑140 (MQ=255)

                                                      aCCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATccc                                                                                >  1:46542/1‑140 (MQ=255)

                                                      aCCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATccc                                                                                >  2:604999/1‑140 (MQ=255)

                                                      aCCGATAACCATCAGCTTCCTCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGTGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATccc                                                                                >  2:393060/1‑140 (MQ=255)

                                                        cGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAg                                                                              >  1:46756/1‑140 (MQ=255)

                                                        cGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAg                                                                              <  2:756723/140‑1 (MQ=255)

                                                          aTAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGgc                                                                            >  1:667250/1‑140 (MQ=255)

                                                            aaCCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGgcgc                                                                          >  2:175597/1‑140 (MQ=255)

                                                            aaCCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGgcgc                                                                          >  1:202559/1‑140 (MQ=255)

                                                            aaCCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGgcgc                                                                          >  2:451599/1‑140 (MQ=255)

                                                                 tCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGtttt                                                                     >  2:424015/1‑140 (MQ=255)

                                                                  cAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGttttt                                                                    >  1:257775/1‑140 (MQ=255)

                                                                       tGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCg                                                               >  1:66613/1‑140 (MQ=255)

                                                                       tGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCg                                                               <  2:66613/140‑1 (MQ=255)

                                                                        gCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGa                                                              >  2:190881/1‑140 (MQ=255)

                                                                         cccTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGaa                                                             >  2:465205/1‑140 (MQ=255)

                                                                             cACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTCCGCCAGCGCGTTTTTATCCCCGGCGCGTTTTTCGCCGAAGgcc                                                         <  2:369488/140‑1 (MQ=255)

                                                                             cACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGgcc                                                         >  2:1011218/1‑140 (MQ=255)

                                                                             cACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGgcc                                                         <  1:945838/140‑1 (MQ=255)

                                                                               ccGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGgccag                                                       >  2:478889/1‑140 (MQ=255)

                                                                                cGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGgccagc                                                      <  1:175597/140‑1 (MQ=255)

                                                                                cGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGgccagc                                                      <  1:296816/140‑1 (MQ=255)

                                                                                     tGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCagta                                                 <  2:257775/140‑1 (MQ=255)

                                                                                     tGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCagta                                                 <  2:459027/140‑1 (MQ=255)

                                                                                          cAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGtttt                                            >  1:889149/1‑140 (MQ=255)

                                                                                          cAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGtttt                                            <  2:202559/140‑1 (MQ=255)

                                                                                            gCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTa                                          >  1:48348/1‑140 (MQ=255)

                                                                                                     aCGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTAcgc                                 <  1:1044817/140‑1 (MQ=255)

                                                                                                      cGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTAcgcg                                <  1:520738/140‑1 (MQ=255)

                                                                                                            gTTAAAGGTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCa                          >  2:1207914/1‑140 (MQ=255)

                                                                                                                   gTGGCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCgg                   <  1:759401/140‑1 (MQ=255)

                                                                                                                      gCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTAcg                                  >  2:1251388/1‑122 (MQ=255)

                                                                                                                      gCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTAcg                                  <  1:1251388/122‑1 (MQ=255)

                                                                                                                      gCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGaa                >  1:546417/1‑140 (MQ=255)

                                                                                                                      gCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGaa                >  2:861783/1‑140 (MQ=255)

                                                                                                                      gCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGaa                >  2:834088/1‑140 (MQ=255)

                                                                                                                      gCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGaa                >  1:15972/1‑140 (MQ=255)

                                                                                                                      gCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGaa                >  2:237314/1‑140 (MQ=255)

                                                                                                                      gCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGaa                >  2:263578/1‑140 (MQ=255)

                                                                                                                      gCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGaa                >  1:505335/1‑140 (MQ=255)

                                                                                                                      gCGGTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGGTTTTATCATTACGCGCCATCAGAATCGGGaa                >  1:338273/1‑140 (MQ=255)

                                                                                                                         gTATCGCGCGTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGAAGGc             <  1:304305/140‑1 (MQ=255)

                                                                                                                              gcgcgTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGAAGGCCGCGt        <  1:861783/140‑1 (MQ=255)

                                                                                                                              gcgcgTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGAAGGCCGCGt        <  2:46542/140‑1 (MQ=255)

                                                                                                                              gcgcgTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGAAGGCCGCGt        <  2:818975/140‑1 (MQ=255)

                                                                                                                                 cgTAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGAAGGCCGCGTTgg     <  2:48348/140‑1 (MQ=255)

                                                                                                                                   tAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAATAGTTTTTATCATTACGCGCCATCAGAATCGGGAAGGCCGCGTTGgag   <  2:515207/140‑1 (MQ=255)

                                                                                                                                   tAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGAAGGCCGCGTTGgag   <  1:834088/140‑1 (MQ=255)

                                                                                                                                   tAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGAAGGCCGCGTTGgag   <  1:451599/140‑1 (MQ=255)

                                                                                                                                   tAACGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGAAGGCCGCGTTGgag   <  1:698377/140‑1 (MQ=255)

                                                                                                                                    aaCGCTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGAAGGCCGCGTTGgaga  >  2:1150125/1‑140 (MQ=255)

CCCGTTCCAGCTGTAACCGTTCGGGCAACGCAAAATGCTGTTGTAGTGTGGGTTACCGATAACCATCAGTTTGCCCTCACCGACTTGCCCCAGCGAAATAAACGGCAGGTTAAAGGTGGCGGTATCGCGCGTAACGTTTTCCGGCTCAACAAGAGAAGGCGCTTCCGTAATGTACGCCAGCTCGTTTTTATCCCAGGCGCGTTTTTCGCCGAAGGCCAGCCAGTAGTTTTTATCATTACGCGCCATCAGAATCGGGAAGGCCGCGTTGGAGA  >  NC_000913/3117364‑3117635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A