Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	153	60.1	2040486	99.3%	2026202	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,950,194	A→G	Q318R (CAG→CGG)	argA →	N‑acetylglutamate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,950,194	0	A	G	100.0%	75.3 / NA	23	Q318R (CAG→CGG)	argA	N‑acetylglutamate synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (9/14); total (9/14)

GATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGGAA  >  NC_000913/2950058‑2950327

gATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGGgcggca                                                                                                                                    >  2:575742/1‑140 (MQ=255)

gATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAgcggca                                                                                                                                    <  1:287689/140‑1 (MQ=255)

  tGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCGGCAAg                                                                                                                                  <  1:64145/140‑1 (MQ=255)

   ggCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCGGCAAgg                                                                                                                                 <  2:207307/140‑1 (MQ=255)

       cgcTGTTGCAAGAGTTGTTCTCACGCGACTGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAgcggca                                                                                                                                    >  1:118594/1‑133 (MQ=255)

       cgcTGTTGCAAGAGTTGTTCTCACGCGACTGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAgcggca                                                                                                                                    <  2:118594/133‑1 (MQ=255)

       cgcTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAgcggca                                                                                                                                    >  1:215571/1‑133 (MQ=255)

       cgcTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAgcggca                                                                                                                                    <  2:215571/133‑1 (MQ=255)

                  gagTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCg                                                                                                                  <  1:286674/140‑1 (MQ=255)

                    gttgttCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCGGCAAGGTa                                                                                                                               <  2:186891/125‑1 (MQ=255)

                    gttgttCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCGGCAAGGTa                                                                                                                               >  1:186891/1‑125 (MQ=255)

                                                cAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCGGCAAGGGATTCTGGTACGCCGTTCTCGCCAGCAGCTGGGGATGGGAAACGa                                                                                    >  2:517598/1‑140 (MQ=255)

                                                cAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGAATTCTGGGGCTGGTTCGCCCCCTGGAGCGGCCAGGGATTCTGGTCTGCCCGTTTCGCAAGCCGCTGGAGGTGGAAAACGa                                                                                    >  2:564603/1‑140 (MQ=255)

                                                    ttGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACaaa                                                                                <  2:970703/140‑1 (MQ=255)

                                                      gtgATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAAtt                                                                              <  2:854583/140‑1 (MQ=255)

                                                      gtgATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAAtt                                                                              <  1:572776/140‑1 (MQ=255)

                                                                      aGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAgcgc                                                              >  2:77414/1‑140 (MQ=255)

                                                                             tcgtcgCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAaca                                                       <  1:77414/140‑1 (MQ=255)

                                                                                 cgcgCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCGGCAAGGGATTTTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGGTAACACGAc                                                   >  2:524352/1‑140 (MQ=255)

                                                                                                  tatTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCcgcg                                   >  1:593766/1‑139 (MQ=255)

                                                                                                  tatTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCcgcg                                   <  2:593766/139‑1 (MQ=255)

                                                                                                     tGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCGGCAAGGGATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCCGCGctct                               >  1:100526/1‑140 (MQ=255)

                                                                                                         ggTATTCTGGAGTTGATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCCGCGCTCTATcc                           >  2:309325/1‑140 (MQ=255)

                                                                                                              tCTGGAGTTGATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCCGCGCTCTATCCGTTcc                      <  1:309325/140‑1 (MQ=255)

                                                                                                                 ggAGTTGATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCCGCGCTCTATCCGTTcc                      <  2:591548/137‑1 (MQ=255)

                                                                                                                 ggAGTTGATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCCGCGCTCTATCCGTTcc                      >  1:591548/1‑137 (MQ=255)

                                                                                                                 ggAGTTGATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCCGCGCTCTATCCGTTCCCgg                   <  1:564603/140‑1 (MQ=255)

                                                                                                                      tGATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCCGCGCTCTATCCGTTCCCGgaagag              >  1:10094/1‑140 (MQ=255)

                                                                                                                       gATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCCGCGCTCTATCCGtt                        <  2:371188/129‑1 (MQ=255)

                                                                                                                       gATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCCGCGCTCTATCCGtt                        >  1:371188/1‑129 (MQ=255)

                                                                                                                       gATTCGCCCACTGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCCGCGCTCTATCCGTTCCCGgaagaga             >  1:338050/1‑140 (MQ=255)

                                                                                                                                  tGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGGaa  >  2:469067/1‑140 (MQ=255)

                                                                                                                                  tGGAGCGGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGGaa  <  2:100526/140‑1 (MQ=255)

                                                                                                                                     agcggcAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGGaa  <  2:513320/137‑1 (MQ=255)

                                                                                                                                     agcggcAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGGaa  >  1:513320/1‑137 (MQ=255)

GATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGTATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGGAA  >  NC_000913/2950058‑2950327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A