Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 153 60.1 2040486 99.3% 2026202 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,826,614 A→G T469A (ACC→GCC)  recG → ATP‑dependent DNA helicase RecG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,826,6140AG100.0% 159.5 / NA 45T469A (ACC→GCC) recGATP‑dependent DNA helicase RecG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/17);  total (28/17)

TGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGG  >  NC_000913/3826486‑3826749
                                                                                                                                |                                                                                                                                       
tGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCAtt                                                                                                                              >  2:480854/1‑140 (MQ=255)
   ccGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGAc                                                                                                                           >  1:718454/1‑140 (MQ=255)
   ccGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGAc                                                                                                                           >  1:729833/1‑140 (MQ=255)
      ccACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACcgc                                                                                                                        >  1:317903/1‑140 (MQ=255)
      ccACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACcgc                                                                                                                        >  1:915617/1‑140 (MQ=255)
       cACGCCGACCCCCCGCCCGCTGGCAATGACTGCGTATGCCGATCTCGATCCCTGGGTGATAGATGAGCGGCCGCCGGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATCCCCGCCGTGCCGACATCATTGACcgcg                                                                                                                       <  2:729833/140‑1 (MQ=255)
       cACCCCGACCCCCCGCCGGCTGGAAATGCCTGCGTATGCCGATCTCGATCCCGGGGTGATAGAGGAGCTCCCGCCGGGCCGCACGCCAGTGACTACGGTCGCTATCCCTGATACCCGCCGTGCCGACATCATTGACcgcg                                                                                                                       <  2:422577/140‑1 (MQ=255)
              aTCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGcacca                                                                                                                >  2:994594/1‑140 (MQ=255)
               ccccccGCACGCTGCCAATCGCCGCGAAGGCGGATCTCGATACCGCGTTGAAGGATGAGCCCCCCCAAGGCCGCACGCCAGTGACTAGGTTCGTTTTCCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGcaccac                                                                                                               <  2:317903/139‑1 (MQ=255)
                 ccccGCCCGCGGGCAATGAGTGCGTATGCCGATCCGGATCCCTCGGTGATAGATGGCCTGCCGCCGGGCCGCACGCGAGTGACTACGGTCGCTATCCCTGATCCCCGCCGTGCCGACATCATTGACCGCGTGCACCACGc                                                                                                             <  2:51428/140‑1 (MQ=255)
                     gCACGCTGGCAATGCCTGCGTATGCCGATCTCGATCCCTGGGTGATAGATGAGCGGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACACCATTGACCGCGTGCACCACGCCTGc                                                                                                         <  2:281166/140‑1 (MQ=255)
                      cACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTTGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCa                                                                                                        >  1:633021/1‑140 (MQ=255)
                           tGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACt                                                                                                   >  1:322928/1‑140 (MQ=255)
                           tGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACt                                                                                                   >  2:344334/1‑140 (MQ=255)
                                     tGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGgtcgtc                                                                                         >  1:963452/1‑140 (MQ=255)
                                      gCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCa                                                                                        >  1:145676/1‑140 (MQ=255)
                                       ggTATGCCGTTCTCGATCCCTGGGTGATAGATGGGCTCCCGCCGGGCCGCACGCCAGTGACTAGGTTTGCTATTCCTGATCCCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAg                                                                                       <  2:633021/139‑1 (MQ=255)
                                       cGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGgtctgtct                                                                                       >  1:179225/1‑135 (MQ=255)
                                         tATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGc                                                                                     >  1:349237/1‑140 (MQ=255)
                                            gCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATa                                                                                  <  1:301527/140‑1 (MQ=255)
                                              cGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACt                                                                                <  2:718454/140‑1 (MQ=255)
                                                 tctcGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGgtc                                                                                            >  2:543699/1‑125 (MQ=255)
                                                 tctcGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGgtc                                                                                            <  1:543699/125‑1 (MQ=255)
                                                     gATCCCTCGGTGATAGATGAGCTGCCCCCGGGCCGCACGCCAGTGACTCCGGTCGTTTTTCCTGATCCCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTg                                                                         <  2:349237/140‑1 (MQ=255)
                                                          cTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGt                                                                    <  2:963452/140‑1 (MQ=255)
                                                            cGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttg                                                                  >  2:377667/1‑140 (MQ=255)
                                                               tGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttgatt                                                               >  1:400978/1‑140 (MQ=255)
                                                                 aTAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttgattga                                                             <  1:377667/140‑1 (MQ=255)
                                                                   aGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAag                                                           >  2:467723/1‑140 (MQ=255)
                                                                              cgccAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTg                                                >  2:258297/1‑140 (MQ=255)
                                                                              cgccAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTg                                                >  2:382852/1‑140 (MQ=255)
                                                                                       gCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAgcg                                       <  1:382852/140‑1 (MQ=255)
                                                                                       gCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAgcg                                       <  1:258297/140‑1 (MQ=255)
                                                                                        cACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAgcgc                                      >  1:944795/1‑140 (MQ=255)
                                                                                                  aCTACGGTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGa                            >  2:170195/1‑140 (MQ=255)
                                                                                                       ggTCGCTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTa                       >  2:74226/1‑140 (MQ=255)
                                                                                                            cTATTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTgg                  >  2:369721/1‑140 (MQ=255)
                                                                                                              aTTCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGa                <  1:74226/140‑1 (MQ=255)
                                                                                                               ttCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGaa               <  2:145676/140‑1 (MQ=255)
                                                                                                               ttCCTGATACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGaa               <  1:83006/140‑1 (MQ=255)
                                                                                                                     aTACCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTg         >  1:105257/1‑140 (MQ=255)
                                                                                                                       aCCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGaa       >  2:870342/1‑140 (MQ=255)
                                                                                                                       aCCCGCCGTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGaa       >  1:725352/1‑140 (MQ=255)
                                                                                                                         ccgccgTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAAc     >  2:394417/1‑140 (MQ=255)
                                                                                                                            ccgTGCCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTgg  >  1:456235/1‑140 (MQ=255)
                                                                                                                                |                                                                                                                                       
TGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGG  >  NC_000913/3826486‑3826749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A