Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	153	60.1	2040486	99.3%	2026202	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,434,695	C→T	G624E (GGG→GAG)	cpdB ←	2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,434,695	0	C	T	100.0%	112.0 / NA	33	G624E (GGG→GAG)	cpdB	2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/15); total (18/15)

AATAAATGCCGGATGCGGTTGATGATTTATAGGCCGAAAAAGAAGTGTTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCCCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTATCGGTGCTAAACGCCAGTTGTTATCTGCCGCCGGGTGAATTTCCCCCGCACGTT  >  NC_000913/4434573‑4434827

aaTAAATGCCGGATGCGGTTGATGATTTATAGGCCGAAAAAGAAGTGTTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAGAc                                                                                                                     >  1:825011/1‑140 (MQ=255)

  tAAATGCCGGATGCGGTTGATGATTTATAGGCCGAAAAAGAAGTGTTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTctct                                                                                                                              <  2:190082/129‑1 (MQ=255)

  tAAATGCCGGATGCGGTTGATGATTTATAGGCCGAAAAAGAAGTGTTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGACGCGACTTTATTCATCGGATACTGCTCTTTctct                                                                                                                              >  1:190082/1‑129 (MQ=255)

    aaTGCCGGATGCGGTTGATGATTTATAGGCCGAAAAAGAAGTGTTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTg                                                                                                                 <  2:263048/140‑1 (MQ=255)

        ccGGATGCGGTTGATGATTTATAGGCCGAAAAAGAAGTGTTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGc                                                                                                             >  1:817215/1‑140 (MQ=255)

           gATGCGGTTGATGATTTATAGGCCGAAAAAGAAGTGTTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCttt                                                                                                          >  1:458682/1‑140 (MQ=255)

              gCGGTTGATGATTTATAGGCCGAAAAAGAAGTGTTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAGACGCTGCGGCTTTATc                                                                                                       <  2:825011/140‑1 (MQ=255)

              gCGGTTGATGATTTATAGGCCGAAAAAGAAGTGTTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATc                                                                                                       <  2:756711/140‑1 (MQ=255)

                     atgatTTATAGGCCGAAAAAGAAGTGTTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGga                                                                                                >  1:254234/1‑140 (MQ=255)

                            ataGGCCGAAAAAGAAGTGTTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTTATAAACGCTGCGGCTTTATCTGACGGAGAGGttt                                                                                         >  2:41140/1‑140 (MQ=255)

                              aGGCCGAAAAAGAAGTGTTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCg                                                                                       >  2:388355/1‑140 (MQ=255)

                                 ccGAAAAAGAAGTGTTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGaaa                                                                                    <  1:871680/140‑1 (MQ=255)

                                    aaaaaGAAGTGTTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACgg                                                                                 <  2:334231/140‑1 (MQ=255)

                                             tgtTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGt                                                                        <  1:671823/140‑1 (MQ=255)

                                              gtTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGtt                                                                       >  1:441282/1‑140 (MQ=255)

                                                    cttactCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTAt                                                                 >  2:1003248/1‑140 (MQ=255)

                                                      tactCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCg                                                               <  2:868597/140‑1 (MQ=255)

                                                             aTCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTa                                                        >  2:488070/1‑140 (MQ=255)

                                                              tCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTAt                                                       >  2:43905/1‑140 (MQ=255)

                                                                  ccTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTATCGGt                                                   >  1:628932/1‑140 (MQ=255)

                                                                  ccTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTATCGGt                                                   >  2:84128/1‑140 (MQ=255)

                                                                     gATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTATCGGTGCt                                                >  1:140078/1‑140 (MQ=255)

                                                                           tCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTATCGGTGCTAAACGc                                          <  1:84128/140‑1 (MQ=255)

                                                                            cGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTATCGGTGCTAAACGcc                                         <  2:384408/140‑1 (MQ=255)

                                                                            cGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTATCGGTGCTAAACGcc                                         <  2:254234/140‑1 (MQ=255)

                                                                            cGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTATCGGTGCTAAACGc                                          >  1:143237/1‑139 (MQ=255)

                                                                            cGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTATCGGTGCTAAACGc                                          <  2:143237/139‑1 (MQ=255)

                                                                              cAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTATCGGTGCTAAACGCCAg                                       <  1:168384/140‑1 (MQ=255)

                                                                                aaCCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTATCGGTGCTAAACGCCAgtt                                     >  2:749804/1‑140 (MQ=255)

                                                                                            tcGGTCGCGACTTTATTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTGTCTTATCGCCAGCTATCGGTGCTAAACGCCAGTTGTTATCTgccgt                         <  2:441282/140‑2 (MQ=255)

                                                                                                          aTTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTATCGGTGCTAAACGCCAGTTGTTATCTGCCGCCGGGTGAATTTccc           >  1:829147/1‑140 (MQ=255)

                                                                                                          aTTCATCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTATCGGTGCTAAACGCCAGTTGTTATCTGCCGCCGGGTGAATTTccc           >  2:984379/1‑140 (MQ=255)

                                                                                                               tCGGATACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTATCGGTGCTAAACGCCAGTTGTTATCTGCCGCCGGGTGAATTTCCCCCGCa      <  1:43905/140‑1 (MQ=255)

                                                                                                                   aTACTGCTCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTATCGGTGCTAAACGCCAGTTGTTATCTGCCGCCGGGTGAATTTCCCCCGCACGtt  >  2:770530/1‑140 (MQ=255)

AATAAATGCCGGATGCGGTTGATGATTTATAGGCCGAAAAAGAAGTGTTTTACTTACTCAAATCCACCTGATAAATCGCAAACCCGATATCATCGGTCGCGACTTTATTCATCGGATACTGCCCTTTCTCTTTAATAAACGCTGCGGCTTTATCTGACGGAGAGGTTTCGAAACGGATATCCAGTTTCTTATCGCCAGCTATCGGTGCTAAACGCCAGTTGTTATCTGCCGCCGGGTGAATTTCCCCCGCACGTT  >  NC_000913/4434573‑4434827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A