Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	151	83.1	2798978	99.4%	2782184	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,709,561	(C)_6→5	coding (915/1692 nt)	eptB ←	Kdo2‑lipid A phosphoethanolamine 7''‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,709,556	0	C	.	98.8%	345.5 / ‑2.4	82	coding (920/1692 nt)	eptB	Kdo2‑lipid A phosphoethanolamine 7''‑transferase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (39/42); total (39/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

GCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACCCATA  >  NC_000913/3709418‑3709690

gCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGCACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcc                                                                                                                                       >  2:955761/1‑140 (MQ=255)

gCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcc                                                                                                                                       <  2:304213/140‑1 (MQ=255)

gCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcc                                                                                                                                       >  1:1375141/1‑140 (MQ=255)

  aaTGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcccc                                                                                                                                     <  1:1074307/140‑1 (MQ=255)

  aaTGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcccc                                                                                                                                     <  1:82917/140‑1 (MQ=255)

  aaTGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcccc                                                                                                                                     <  1:1393535/140‑1 (MQ=255)

   aTGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccc                                                                                                                                    <  1:239599/140‑1 (MQ=255)

    tgttgtCCGCCATCGTG‑TGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccctg                                                                                                                                  <  2:1371789/140‑3 (MQ=255)

     gttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccctg                                                                                                                                  >  2:470684/1‑138 (MQ=255)

     gttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccctg                                                                                                                                  <  1:552009/140‑3 (MQ=255)

     gttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccctg                                                                                                                                  >  1:308237/1‑138 (MQ=255)

     gttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcccc                                                                                                                                     >  2:229439/1‑137 (MQ=255)

     gttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcccc                                                                                                                                     <  1:229439/137‑1 (MQ=255)

       tgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccctgac                                                                                                                                >  2:582306/1‑136 (MQ=255)

         tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGt                                                                                                                             >  2:1074314/1‑140 (MQ=255)

         tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGt                                                                                                                             >  2:883679/1‑140 (MQ=255)

         tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGt                                                                                                                             >  1:1175956/1‑140 (MQ=255)

         tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGt                                                                                                                             >  2:602177/1‑140 (MQ=255)

            gCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CACCCTGACGTACa                                                                                                                          <  2:92280/140‑1 (MQ=255)

             ccATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACaa                                                                                                                         <  2:1001574/140‑1 (MQ=255)

              cATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACaaa                                                                                                                        >  1:684462/1‑140 (MQ=255)

                   tgtTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGc                                                                                                                   <  2:575717/140‑1 (MQ=255)

                    gtTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCa                                                                                                                  <  1:157620/140‑1 (MQ=255)

                       gCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACg                                                                                                               <  2:1043005/140‑1 (MQ=255)

                           tAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAATATGCAACGCAGt                                                                                                           <  1:190805/140‑1 (MQ=255)

                             gAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACg                                                                                                               >  1:781695/1‑134 (MQ=255)

                             gAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACg                                                                                                               <  2:781695/134‑1 (MQ=255)

                             gAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTga                                                                                                         >  1:222263/1‑140 (MQ=255)

                             gAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTga                                                                                                         >  2:838156/1‑140 (MQ=255)

                                ccACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGagag                                                                                                      >  1:54597/1‑140 (MQ=255)

                                ccACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGagag                                                                                                      >  1:330107/1‑140 (MQ=255)

                                 cacaTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGTATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGt                                                                                                     <  2:131269/140‑1 (MQ=255)

                                  acaTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGtt                                                                                                    >  2:244885/1‑140 (MQ=255)

                                    aTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTg                                                                                                  <  1:838156/140‑1 (MQ=255)

                                      ttCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                                                                <  1:747862/140‑1 (MQ=255)

                                       tCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGtt                                                                                               <  2:120767/140‑1 (MQ=255)

                                        cGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTg                                                                                              <  1:181714/140‑1 (MQ=255)

                                           tctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                           <  2:1102037/140‑1 (MQ=255)

                                           tctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                           <  1:244885/140‑1 (MQ=255)

                                           tctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                           <  2:222263/140‑1 (MQ=255)

                                           tctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                           <  1:298882/140‑1 (MQ=255)

                                            ctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGt                                                                                          <  2:54597/140‑1 (MQ=255)

                                               cATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATc                                                                                       <  1:883679/140‑1 (MQ=255)

                                                aTAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca                                                                                      <  2:330107/140‑1 (MQ=255)

                                                 tAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac                                                                                     <  2:1143265/140‑1 (MQ=255)

                                                    cGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc                                                                                             >  1:140699/1‑129 (MQ=255)

                                                    cGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc                                                                                             <  2:140699/129‑1 (MQ=255)

                                                    cGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAgg                                                                                  >  2:1026728/1‑140 (MQ=255)

                                                    cGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAgg                                                                                  >  2:36441/1‑140 (MQ=255)

                                                      tAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAg                                                                                <  2:684462/140‑1 (MQ=255)

                                                       agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGt                                                                               >  1:110275/1‑140 (MQ=255)

                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTaa                                                                             >  2:515389/1‑140 (MQ=255)

                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTaa                                                                             >  2:493868/1‑140 (MQ=255)

                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTaa                                                                             <  2:308237/140‑1 (MQ=255)

                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTaa                                                                             >  2:1181563/1‑140 (MQ=255)

                                                           gTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAcc                                                                           >  2:218556/1‑140 (MQ=255)

                                                              aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACg                                                                        >  1:911693/1‑140 (MQ=255)

                                                                         cTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCa                                                             <  1:955761/140‑1 (MQ=255)

                                                                                cTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAttttt                                                      >  1:629937/1‑140 (MQ=255)

                                                                                cTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAttttt                                                      >  1:650919/1‑140 (MQ=255)

                                                                                    aGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTcc                                                  <  1:1216389/140‑1 (MQ=255)

                                                                                        ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg                                              >  1:245450/1‑140 (MQ=255)

                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg                                              >  1:747264/1‑137 (MQ=255)

                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg                                              <  2:747264/137‑1 (MQ=255)

                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCa                                           >  2:137611/1‑140 (MQ=255)

                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCa                                           >  2:1005464/1‑140 (MQ=255)

                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAATGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCa                                           >  1:746380/1‑140 (MQ=255)

                                                                                               aaTGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGttt                                       <  2:383085/140‑1 (MQ=255)

                                                                                               aaTGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGttt                                       <  1:1005464/140‑1 (MQ=255)

                                                                                                   ttctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGc                                   >  1:354308/1‑140 (MQ=255)

                                                                                                    tctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCC‑GACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                                  <  2:1298379/140‑1 (MQ=255)

                                                                                                      tgttctTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTg                                <  1:1026728/140‑1 (MQ=255)

                                                                                                           ttttAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTAtt                           <  1:602177/140‑1 (MQ=255)

                                                                                                             ttAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                                  >  2:936759/1‑131 (MQ=255)

                                                                                                             ttAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                                  <  1:936759/131‑1 (MQ=255)

                                                                                                             ttAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTAc                         >  2:301423/1‑140 (MQ=255)

                                                                                                                  gtgCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa                    >  2:197020/1‑140 (MQ=255)

                                                                                                                  gtgCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa                    <  1:301423/140‑1 (MQ=255)

                                                                                                                  gtgCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa                    >  2:827437/1‑140 (MQ=255)

                                                                                                                  gtgCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa                    <  1:36441/140‑1 (MQ=255)

                                                                                                                  gtgCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa                    >  1:974191/1‑140 (MQ=255)

                                                                                                                  gtgCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa                    >  2:100173/1‑140 (MQ=255)

                                                                                                                  gtgCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa                    >  2:1174355/1‑140 (MQ=255)

                                                                                                                     cgcTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAg                 >  2:903100/1‑140 (MQ=255)

                                                                                                                      gcTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGc                <  2:1375141/140‑1 (MQ=255)

                                                                                                                       cTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGcc               >  1:440031/1‑140 (MQ=255)

                                                                                                                         gCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGa             <  1:272087/140‑1 (MQ=255)

                                                                                                                            gATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGaaaa          <  1:903100/140‑1 (MQ=255)

                                                                                                                            gATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGaaaa          <  1:515389/140‑1 (MQ=255)

                                                                                                                             aTTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAAt         >  2:150072/1‑140 (MQ=255)

                                                                                                                              ttATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATa        <  1:1181563/140‑1 (MQ=255)

                                                                                                                              ttATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATa        <  1:1074314/140‑1 (MQ=255)

                                                                                                                                  cTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAAt         <  2:433674/135‑1 (MQ=255)

                                                                                                                                  cTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAAt         >  1:433674/1‑135 (MQ=255)

                                                                                                                                    tCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACCCata  <  2:110275/140‑1 (MQ=255)

                                                                                                                                    tCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACCCata  <  2:1175956/140‑1 (MQ=255)

GCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACCCATA  >  NC_000913/3709418‑3709690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A