Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R1	151	83.1	2798978	99.4%	2782184	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,993,461	A→G	H770R (CAC→CGC)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,993,461	0	A	G	100.0%	184.8 / NA	51	H770R (CAC→CGC)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/26); total (25/26)

ATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGA  >  NC_000913/3993326‑3993596

aTCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATcgccac                                                                                                                                     >  1:1105960/1‑140 (MQ=255)

aTCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAAAGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATcgccac                                                                                                                                     >  1:260811/1‑140 (MQ=255)

   aCGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGc                                                                                                                                  <  2:603801/140‑1 (MQ=255)

     gTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGa                                                                                                                                <  2:370986/140‑1 (MQ=255)

     gTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGa                                                                                                                                <  1:85596/140‑1 (MQ=255)

          aTTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGgca                                                                                                                           <  2:828851/140‑1 (MQ=255)

                ggcggTGGGGGGCGGCTTTGCCAGCGAAGGGGTCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTTATATCTACATTCTCGACGAAAGCAACCGGGGTGAGGGATATCGCCACTGCGAAGGCAGCaaaa                                                                                                                     >  2:746453/1‑139 (MQ=255)

                    gtggtgGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAgagga                                                                                                                 <  1:1281262/140‑1 (MQ=255)

                    gtggtgGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAAACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGGATATCGCCACTGCGAAGGCAGCAAAgaggg                                                                                                                 >  2:791413/1‑139 (MQ=255)

                     tggtggACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAgaggag                                                                                                                <  2:145052/140‑1 (MQ=255)

                       gtggACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCt                                                                                                              <  1:1317876/140‑1 (MQ=255)

                        tggACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTg                                                                                                             >  1:1090342/1‑140 (MQ=255)

                              gCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGt                                                                                                       >  2:1108239/1‑140 (MQ=255)

                               cTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTg                                                                                                      >  1:933109/1‑140 (MQ=255)

                               cTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTg                                                                                                      >  2:432437/1‑140 (MQ=255)

                                 ttGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGAc                                                                                                    <  2:1362863/140‑1 (MQ=255)

                                 ttGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGAc                                                                                                    >  1:1327532/1‑140 (MQ=255)

                                  tGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACg                                                                                                   <  2:1105960/140‑1 (MQ=255)

                                  tGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACg                                                                                                   <  2:1053747/140‑1 (MQ=255)

                                       gCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGt                                                                                              <  1:1380656/140‑1 (MQ=255)

                                                    ccAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGt                                                                                 <  2:257635/140‑1 (MQ=255)

                                                        ttCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATc                                                                             >  1:39019/1‑140 (MQ=255)

                                                        ttCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATc                                                                             >  1:447930/1‑140 (MQ=255)

                                                                cGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGAcc                                                                     <  1:432437/140‑1 (MQ=255)

                                                                  aagaaACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGt                                                                   >  2:884935/1‑140 (MQ=255)

                                                                  aagaaACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGt                                                                   >  2:605816/1‑140 (MQ=255)

                                                                                     tGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCt                                                <  2:260811/140‑1 (MQ=255)

                                                                                       gCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTtc                                              >  2:1373423/1‑140 (MQ=255)

                                                                                        cTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTtca                                             <  2:950724/140‑1 (MQ=255)

                                                                                            aaTATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCaa                                         >  2:562499/1‑140 (MQ=255)

                                                                                                 cTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCa                                    >  2:900927/1‑140 (MQ=255)

                                                                                                 cTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCa                                    >  1:345154/1‑140 (MQ=255)

                                                                                                 cTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCa                                    >  2:994296/1‑140 (MQ=255)

                                                                                                  tACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTtcat                                            <  2:1301344/131‑1 (MQ=255)

                                                                                                  tACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTtcat                                            >  1:1301344/1‑131 (MQ=255)

                                                                                                  tACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCaa                                   >  1:208130/1‑140 (MQ=255)

                                                                                                  tACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCaa                                   >  1:284782/1‑140 (MQ=255)

                                                                                                   aCATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAAc                                  >  1:582761/1‑140 (MQ=255)

                                                                                                   aCATTCTCGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAAc                                  <  1:605816/140‑1 (MQ=255)

                                                                                                       tctcGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGc                              >  2:305576/1‑140 (MQ=255)

                                                                                                       tctcGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGc                              >  1:165185/1‑140 (MQ=255)

                                                                                                       tctcGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGc                              >  2:690577/1‑140 (MQ=255)

                                                                                                        ctcGACGAAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGcc                             <  1:884935/140‑1 (MQ=255)

                                                                                                          cgacgaAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGc                           <  2:1090342/140‑1 (MQ=255)

                                                                                                          cgacgaAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGc                           <  2:582761/140‑1 (MQ=255)

                                                                                                          cgacgaAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGc                           <  1:746453/140‑1 (MQ=255)

                                                                                                              gaAAGCAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGtt                       >  1:1393227/1‑140 (MQ=255)

                                                                                                                   cAACCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATc                  >  1:733653/1‑140 (MQ=255)

                                                                                                                    aaCCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCa                 <  1:228945/140‑1 (MQ=255)

                                                                                                                    aaCCGGGTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCa                 <  1:305576/140‑1 (MQ=255)

                                                                                                                          gTTGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATtgt           >  1:1378487/1‑140 (MQ=255)

                                                                                                                           ttGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATtgtg          >  1:87901/1‑140 (MQ=255)

                                                                                                                           ttGAGGTATATCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATtgtg          <  2:208130/140‑1 (MQ=255)

                                                                                                                                   tatCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGa  <  1:791413/140‑1 (MQ=255)

                                                                                                                                   tatCGCCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGa  <  2:733653/140‑1 (MQ=255)

ATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGA  >  NC_000913/3993326‑3993596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A