Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F28 I1 R1 151 83.1 2798978 99.4% 2782184 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,592,406 Δ1 bp coding (750/1656 nt) tsr → methyl‑accepting chemotaxis protein Tsr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,592,4030G.98.6% 284.9 / ‑2.0 69coding (747/1656 nt)tsrmethyl‑accepting chemotaxis protein Tsr
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base . (29/39);  minor base T (0/1);  total (29/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

TGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGC  >  NC_000913/4592267‑4592536
                                                                                                                                        |                                                                                                                                     
tGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCCATGAATCGCCTGATTGACAGCATTCGTCATATTTCAGGCGGGGATCTGGTGAAAACCATTGAGGGGGATGGCTCTAATGAGATgggg                                                                                                                                    >  2:770953/1‑140 (MQ=255)
  gCGGTCATCTTCGCCGTCTGGGTCGGGATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATgggcaa                                                                                                                                  >  2:124123/1‑137 (MQ=255)
    ggTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACt                                                                                                                               >  2:1212611/1‑140 (MQ=255)
     gTCATCTTCGCCGTCTGGGTCGGTATTAAAGCCTCGCTGGTAGCGCCCATGAAACGCCTGGTTTACAGCATTCGTCATATTGCAGGCGGCGATCTGGGGGAACCGATTGAGGGGGATGGGTCTAATGAGAT‑GGGCAACTg                                                                                                                              >  2:927834/1‑140 (MQ=255)
     gTCATCTTCGCCGTCTGGGTCGGGATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTg                                                                                                                              >  2:770801/1‑140 (MQ=255)
         tCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCag                                                                                                                          <  2:84568/140‑1 (MQ=255)
         tCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCag                                                                                                                          >  1:141030/1‑140 (MQ=255)
                  tCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTgc                                                                                                                 <  1:814847/140‑1 (MQ=255)
                   cTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTgcg                                                                                                                <  1:1281421/140‑1 (MQ=255)
                    tGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTgcgc                                                                                                               <  1:875401/140‑1 (MQ=255)
                    tGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTgcgc                                                                                                               <  1:927834/140‑1 (MQ=255)
                      gTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCa                                                                                                             <  1:1056747/140‑1 (MQ=255)
                      gTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCa                                                                                                             <  2:666008/140‑1 (MQ=255)
                       ttCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCa                                                                                                             >  2:628154/1‑139 (MQ=255)
                       ttCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCa                                                                                                             <  1:628154/139‑1 (MQ=255)
                              ttAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAgg                                                                                                     <  2:1173839/140‑1 (MQ=255)
                                   gCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTGATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGagag                                                                                                <  1:1214281/140‑1 (MQ=255)
                                     cTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCt                                                                                              <  1:1270019/140‑1 (MQ=255)
                                      tCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTg                                                                                             <  1:942788/140‑1 (MQ=255)
                                      tCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTg                                                                                             >  2:688574/1‑140 (MQ=255)
                                      tCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTg                                                                                             >  1:355265/1‑140 (MQ=255)
                                      tCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTg                                                                                             >  1:676023/1‑140 (MQ=255)
                                            gTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGt                                                                                       <  1:770801/140‑1 (MQ=255)
                                            gTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGt                                                                                       <  1:688574/140‑1 (MQ=255)
                                             tAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTa                                                                                      <  1:124123/140‑1 (MQ=255)
                                              aGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTAc                                                                                     >  2:6498/1‑140 (MQ=255)
                                               gcgcCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTAcc                                                                                    >  1:812367/1‑140 (MQ=255)
                                                 gcCAATGAATCGCCTGATTGACAGCACTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGt                                                                                  <  1:978781/140‑1 (MQ=255)
                                                  ccAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTc                                                                                 >  2:763930/1‑140 (MQ=255)
                                                  ccAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGAAGCGTACCGTc                                                                                 >  2:1350779/1‑140 (MQ=255)
                                                   cAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCg                                                                                <  1:344498/140‑1 (MQ=255)
                                                     aTGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGt                                                                              >  1:1064390/1‑140 (MQ=255)
                                                      tGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTg                                                                             <  2:166258/140‑1 (MQ=255)
                                                          tCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGAtgt                                                                         >  1:903129/1‑140 (MQ=255)
                                                             ccTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTgcg                                                                      >  1:623834/1‑140 (MQ=255)
                                                             ccTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTgcg                                                                      >  1:869883/1‑140 (MQ=255)
                                                             ccTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTgcg                                                                      >  1:19509/1‑140 (MQ=255)
                                                                 aTTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAAc                                                                  <  1:1212611/140‑1 (MQ=255)
                                                                     aCAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACgggg                                                              <  2:623834/140‑1 (MQ=255)
                                                                     aCAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACgggg                                                              <  2:869883/140‑1 (MQ=255)
                                                                      cAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGc                                                             <  1:931538/140‑1 (MQ=255)
                                                                      cAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGc                                                             <  1:1161118/140‑1 (MQ=255)
                                                                      cAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGc                                                             <  1:606480/140‑1 (MQ=255)
                                                                      cAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAG‑TTGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGc                                                             <  1:1155424/140‑1 (MQ=255)
                                                                        gCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCa                                                           <  2:355265/140‑1 (MQ=255)
                                                                           ttCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATg                                                        >  2:522987/1‑140 (MQ=255)
                                                                           ttCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATg                                                        <  2:812367/140‑1 (MQ=255)
                                                                           ttCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATg                                                        <  1:1107956/140‑1 (MQ=255)
                                                                             cgccATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGcc                                                      <  1:697649/137‑1 (MQ=255)
                                                                             cGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGcc                                                      <  1:489770/140‑1 (MQ=255)
                                                                             cGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGcc                                                      <  1:724786/140‑1 (MQ=255)
                                                                              gTCATAGTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCAGATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCa                                                     >  2:319738/1‑140 (MQ=255)
                                                                               tCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCAt                                                    <  1:64798/140‑1 (MQ=255)
                                                                                         ggcggcGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATg                                                        >  2:294544/1‑126 (MQ=255)
                                                                                         ggcggcGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATg                                                        <  1:294544/126‑1 (MQ=255)
                                                                                            ggcgATCTGGTGGAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGcc                                                      <  1:1117118/125‑1 (MQ=255)
                                                                                            ggcgATCTGGTGGAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGcc                                                      >  2:1117118/1‑125 (MQ=255)
                                                                                            ggcgATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATg                                                        <  2:570868/123‑1 (MQ=255)
                                                                                            ggcgATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATg                                                        >  1:570868/1‑123 (MQ=255)
                                                                                              cgATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAg                                     >  2:57001/1‑140 (MQ=255)
                                                                                               gATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGc                                    <  2:903129/140‑1 (MQ=255)
                                                                                                          aCCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCAc                         >  2:941909/1‑140 (MQ=255)
                                                                                                          aCCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCAc                         <  1:770953/140‑1 (MQ=255)
                                                                                                          aCCGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCAc                         >  1:450198/1‑140 (MQ=255)
                                                                                                           ccGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCAcc                        <  2:1317935/140‑1 (MQ=255)
                                                                                                            cGATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCg                       >  1:1313374/1‑140 (MQ=255)
                                                                                                             gATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCgg                      <  1:763930/140‑1 (MQ=255)
                                                                                                             gATTGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCgg                      >  1:1206138/1‑140 (MQ=255)
                                                                                                               ttGAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCa                    >  2:181223/1‑140 (MQ=255)
                                                                                                                 gAGGTGGATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAAt                  <  2:19509/140‑1 (MQ=255)
                                                                                                                       gATGGCTCTAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGAt            >  1:106427/1‑140 (MQ=255)
                                                                                                                            ctctAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGAtctctc       >  2:1178511/1‑140 (MQ=255)
                                                                                                                               tAATGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTc    >  2:1120994/1‑140 (MQ=255)
                                                                                                                                 aTGAGAT‑GGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTcgc  >  2:899512/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                     
TGGCGGTCATCTTCGCCGTCTGGTTCGGTATTAAAGCCTCGCTGGTAGCGCCAATGAATCGCCTGATTGACAGCATTCGTCATATTGCAGGCGGCGATCTGGTGAAACCGATTGAGGTGGATGGCTCTAATGAGATGGGGCAACTGGCAGAGAGTTTGCGCCATATGCAGGGAGAGCTGATGCGTACCGTCGGTGATGTGCGCAACGGGGCCAATGCCATCTATAGCGGTGCCAGCGAAATCGCCACCGGCAATAACGATCTCTCTTCGC  >  NC_000913/4592267‑4592536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A