Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	138	91.1	3071990	99.2%	3047414	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,822,059	T→C	intergenic (‑48/+80)	alaS ← / ← recX	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor/RecA inhibitor RecX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,822,059	0	T	C	100.0%	237.9 / NA	70	intergenic (‑48/+80)	alaS/recX	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor/RecA inhibitor RecX
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (39/31); total (39/31)

CCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGTGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGATATCTTCCATCAGATAGCCACGATA  >  NC_000913/2821924‑2822195

ccAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCt                                                                                                                                      >  1:20740/1‑140 (MQ=255)

ccAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCt                                                                                                                                      >  1:1370316/1‑140 (MQ=255)

ccAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCt                                                                                                                                      >  1:1333307/1‑140 (MQ=255)

        ctgctgGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAAccgcc                                                                                                                              >  2:1109335/1‑140 (MQ=255)

        ctgctgGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAAccgcc                                                                                                                              >  2:491589/1‑140 (MQ=255)

        ctgctgGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAAccgcc                                                                                                                              >  1:1198536/1‑140 (MQ=255)

          gctgGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCgg                                                                                                                            >  1:1147954/1‑140 (MQ=255)

           ctgGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCggg                                                                                                                           >  1:500374/1‑140 (MQ=255)

           ctgGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCggg                                                                                                                           <  2:20740/140‑1 (MQ=255)

             ggCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCt                                                                                                                         <  2:1198536/140‑1 (MQ=255)

              gCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCtt                                                                                                                                     >  1:718387/1‑127 (MQ=255)

              gCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCtt                                                                                                                                     <  2:718387/127‑1 (MQ=255)

                   tACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAAc                                                                                                                   >  1:286379/1‑140 (MQ=255)

                     ccTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTg                                                                                                                 <  2:286379/140‑1 (MQ=255)

                       tGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGaa                                                                                                               <  2:193185/140‑1 (MQ=255)

                            tCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTg                                                                                                          <  2:1333307/140‑1 (MQ=255)

                               cTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCTACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGa                                                                                                       >  2:1125005/1‑140 (MQ=255)

                               cTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGa                                                                                                       >  2:469639/1‑140 (MQ=255)

                                ttACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGaa                                                                                                      <  1:491589/140‑1 (MQ=255)

                                ttACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGaa                                                                                                      >  2:506066/1‑140 (MQ=255)

                                 tACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAat                                                                                                     <  1:1109335/140‑1 (MQ=255)

                                    tATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAataag                                                                                                  <  2:500374/140‑1 (MQ=255)

                                      tGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAataagat                                                                                                >  2:59748/1‑140 (MQ=255)

                                      tGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAataagat                                                                                                >  2:212417/1‑140 (MQ=255)

                                      tGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAataagat                                                                                                >  2:441526/1‑140 (MQ=255)

                                        gAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAataagataa                                                                                              <  2:836331/140‑1 (MQ=255)

                                        gAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAataagataa                                                                                              <  2:509979/140‑1 (MQ=255)

                                              gTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGTGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTc                                                                                        >  1:1012768/1‑140 (MQ=255)

                                                  agaAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGa                                                                                    <  1:710053/140‑1 (MQ=255)

                                                     aaCGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTg                                                                                 <  2:1147954/140‑1 (MQ=255)

                                                       cGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTggg                                                                               <  1:59748/140‑1 (MQ=255)

                                                       cGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTggg                                                                               <  1:496936/140‑1 (MQ=255)

                                                         ccTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTggg                                                                               <  2:1467883/138‑1 (MQ=255)

                                                         ccTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTggg                                                                               >  1:1467883/1‑138 (MQ=255)

                                                         ccTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGaa                                                                             >  2:1146422/1‑140 (MQ=255)

                                                         ccTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGaa                                                                             >  1:257740/1‑140 (MQ=255)

                                                               ggATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCTACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTaaaa                                                                       <  1:1125005/140‑1 (MQ=255)

                                                                    tcAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCg                                                                  <  1:1146422/140‑1 (MQ=255)

                                                                      aGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTggg                                                                               >  2:1501577/1‑125 (MQ=255)

                                                                      aGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTggg                                                                               <  1:1501577/125‑1 (MQ=255)

                                                                      aGCGGTGCTCTTGCTCACAATTATCCGGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGGGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTa                                                                <  1:1062904/140‑1 (MQ=255)

                                                                           tGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGt                                                           >  2:1158707/1‑140 (MQ=255)

                                                                            gCTCTTGCTTAGAATTATCCTGAAAGCAAGCTAACGAAATATCGCCGCCAGCTCCAGTGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGtt                                                          <  2:1012768/140‑1 (MQ=255)

                                                                             ctctTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTc                                                         >  2:900717/1‑140 (MQ=255)

                                                                             ctctTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTc                                                         >  2:87053/1‑140 (MQ=255)

                                                                                     cATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCa                                                 >  1:1230635/1‑140 (MQ=255)

                                                                                         aTTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAAt                                             <  1:506066/140‑1 (MQ=255)

                                                                                             tCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCg                                         >  2:1160830/1‑140 (MQ=255)

                                                                                                tGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCa                                      >  1:5468/1‑140 (MQ=255)

                                                                                                tGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCa                                      >  2:857076/1‑140 (MQ=255)

                                                                                                   aaTCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAAt                                   <  1:469639/140‑1 (MQ=255)

                                                                                                   aaTCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAAt                                   <  2:749591/140‑1 (MQ=255)

                                                                                                    aTCAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAAtc                                  >  2:1058122/1‑140 (MQ=255)

                                                                                                      cAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAAtctc                                <  1:1058122/140‑1 (MQ=255)

                                                                                                      cAAGCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAAtctc                                <  1:900717/140‑1 (MQ=255)

                                                                                                         gCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTg                             >  2:452843/1‑140 (MQ=255)

                                                                                                         gCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTG‑AAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCaa                                     >  2:414692/1‑131 (MQ=255)

                                                                                                         gCTAACGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTG‑AAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCaa                                     <  1:414692/131‑1 (MQ=255)

                                                                                                            aaCGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGat                          >  2:737544/1‑140 (MQ=255)

                                                                                                             aCGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGata                         <  1:87053/140‑1 (MQ=255)

                                                                                                              cGAAATATCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGatat                        >  2:533632/1‑140 (MQ=255)

                                                                                                                   tatCGCTACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGATATCTTcc                   >  2:1415063/1‑140 (MQ=255)

                                                                                                                   tatCGCCACCAGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGAAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGATATCTTcc                   >  2:813642/1‑140 (MQ=255)

                                                                                                                        ccaccaGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGTATAAGATAACTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGATATCTTCCATCAg              <  2:1370316/140‑1 (MQ=255)

                                                                                                                        ccaccaGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGATATCTTCCATCAg              <  2:1101973/140‑1 (MQ=255)

                                                                                                                         caccaGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGATATCTTCCATCAGa             <  1:441526/140‑1 (MQ=255)

                                                                                                                          accaGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGATATCTTCCATCAGAt            >  1:385301/1‑140 (MQ=255)

                                                                                                                           ccaGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGATATCTTCCATCAGATa           >  2:68693/1‑140 (MQ=255)

                                                                                                                           ccaGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGATATCTTCCATCAGATa           >  2:400630/1‑140 (MQ=255)

                                                                                                                            caGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGATATCTTCCATCAGATAg          >  1:1465670/1‑140 (MQ=255)

                                                                                                                             aGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGATATCTTCCATCAGATAGc         <  1:737544/140‑1 (MQ=255)

                                                                                                                             aGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGATATCTTCCATCAGATAGc         <  1:452843/140‑1 (MQ=255)

                                                                                                                             aGCTCCAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGATATCTTCCATCAGATAGc         <  1:1160830/140‑1 (MQ=255)

                                                                                                                                  cAGCGCGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGATATCTTCCATCAGATAGCCACGa    >  1:982861/1‑140 (MQ=255)

                                                                                                                                    gcgcGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGATATCTTCCATCAGATAGCCACGATa  <  1:1158707/140‑1 (MQ=255)

CCAGGGAGCTGCTGGCAACTACCTGATGTCCCTTACTATGGAAAAAGTCGAGAAACGCCTGACGGATCTCAGCGGTGCTCTTGCTCATAATTATCCTGAAATCAAGCTAACGAAATATCGCCACCAGCTCCAGCGTGTCTTAACCGCCGGGCTGGTAACTGAAAAGTGGGAATAAGATAAGTTTTCTTGACTGGGAAGTAAAATACCGTATGCGTTCAGTCGGCAAAATTTCGCCAAATCTCCTGGATATCTTCCATCAGATAGCCACGATA  >  NC_000913/2821924‑2822195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A