Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F28 I1 R1 138 91.1 3071990 99.2% 3047414 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,974,495 T→C L10P (CTA→CCA)  rffC → dTDP‑4‑amino‑4,6‑dideoxy‑D‑galactose acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,974,4950TC98.7% 254.0 / ‑6.2 74L10P (CTA→CCA) rffCdTDP‑4‑amino‑4,6‑dideoxy‑D‑galactose acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (33/40);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGGAAGAGATTGCCTGGCGTAACGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAA  >  NC_000913/3974357‑3974631
                                                                                                                                          |                                                                                                                                        
cctgGAAGAGATTGCCTGGCGTAACGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTT‑CTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCaa                                                                                                                                        >  1:1229781/1‑140 (MQ=255)
          aTTGCCTGGCGTAACGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGaaa                                                                                                                               >  2:1525218/1‑140 (MQ=255)
            tGCCTGGCGTAACGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAAc                                                                                                                             <  2:720908/140‑1 (MQ=255)
              ccTGGCGTAACGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGc                                                                                                                           >  1:390417/1‑140 (MQ=255)
              ccTGGCGTAACGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGc                                                                                                                           <  2:390417/140‑1 (MQ=255)
                  gCGTAACGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGcc                                                                                                                          <  2:1043460/137‑1 (MQ=255)
                  gCGTAACGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGcc                                                                                                                          >  1:1043460/1‑137 (MQ=255)
                  gCGTAACGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCcttc                                                                                                                       >  1:975821/1‑140 (MQ=255)
                  gCGTAACGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCcttc                                                                                                                       >  1:1000913/1‑140 (MQ=255)
                   cGTAACGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCcttct                                                                                                                      <  1:1275003/140‑1 (MQ=255)
                   cGTAACGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCcttct                                                                                                                      <  1:835310/140‑1 (MQ=255)
                     tAACGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCttt                                                                                                                    <  2:150848/140‑1 (MQ=255)
                     tAACGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCttt                                                                                                                    <  2:226653/140‑1 (MQ=255)
                      aaCGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTg                                                                                                                   <  1:115844/140‑1 (MQ=255)
                        cGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGgt                                                                                                                 <  1:935479/140‑1 (MQ=255)
                        cGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGgt                                                                                                                 <  1:1420782/140‑1 (MQ=255)
                             ggctCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTaa                                                                                                            >  1:597072/1‑140 (MQ=255)
                             ggctCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTaa                                                                                                            <  1:468504/140‑1 (MQ=255)
                             ggctCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTaa                                                                                                            <  1:403947/140‑1 (MQ=255)
                             ggctCGATGACGAGGGCGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGTGTTaa                                                                                                            >  2:937734/1‑140 (MQ=255)
                               ctCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACa                                                                                                          >  1:965498/1‑140 (MQ=255)
                                tCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAg                                                                                                         <  1:584269/140‑1 (MQ=255)
                                    tGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGcc                                                                                                     >  2:1230330/1‑140 (MQ=255)
                                    tGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGcc                                                                                                     >  1:1112656/1‑140 (MQ=255)
                                     gACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAGCAGCGCCa                                                                                                    <  1:1080117/140‑1 (MQ=255)
                                        gAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCg                                                                                                 <  2:97277/140‑1 (MQ=255)
                                               tgAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCAt                                                                                          >  2:146972/1‑140 (MQ=255)
                                                     gTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTgc                                                                                              >  2:824866/1‑130 (MQ=255)
                                                     gTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTgc                                                                                              <  1:824866/130‑1 (MQ=255)
                                                     gTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTc                                                                                    >  1:1315435/1‑140 (MQ=255)
                                                      tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTg                                                                                               >  2:694083/1‑128 (MQ=255)
                                                      tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTg                                                                                               >  2:1155810/1‑128 (MQ=255)
                                                      tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTg                                                                                               <  1:694083/128‑1 (MQ=255)
                                                      tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTg                                                                                               <  1:1155810/128‑1 (MQ=255)
                                                      tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCa                                                                                           <  2:941481/132‑1 (MQ=255)
                                                      tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCa                                                                                           >  1:941481/1‑132 (MQ=255)
                                                      tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCt                                                                                   >  2:173182/1‑140 (MQ=255)
                                                      tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCt                                                                                   >  1:1337513/1‑140 (MQ=255)
                                                      tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCt                                                                                   >  1:149684/1‑140 (MQ=255)
                                                          gcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAg                                                                               <  1:1230330/140‑1 (MQ=255)
                                                           ccAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGc                                                                              <  1:1101127/140‑1 (MQ=255)
                                                                  aTTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTc                                                                       >  2:1442304/1‑140 (MQ=255)
                                                                         aaaaCTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGc                                                                >  1:1130102/1‑140 (MQ=255)
                                                                                gCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGAcgc                                                         >  1:1423745/1‑140 (MQ=255)
                                                                                    cGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTa                                                     <  2:1264102/140‑1 (MQ=255)
                                                                                     ggCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAg                                                    <  2:1337513/140‑1 (MQ=255)
                                                                                       ccAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAgcg                                                  <  1:1525218/140‑1 (MQ=255)
                                                                                              ctgctgGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGt                                           <  1:173182/140‑1 (MQ=255)
                                                                                                gctgGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCa                                         <  2:597072/140‑1 (MQ=255)
                                                                                                 ctgGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCAc                                        >  2:474791/1‑140 (MQ=255)
                                                                                                      gTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTg                                   <  1:146972/140‑1 (MQ=255)
                                                                                                       ttACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGc                                  >  2:614102/1‑140 (MQ=255)
                                                                                                         aCTTCGTGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAg                                >  1:1095698/1‑140 (MQ=255)
                                                                                                               tGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCaaa                          >  2:205669/1‑140 (MQ=255)
                                                                                                               tGCCCGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGAGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCaaa                          >  2:491888/1‑140 (MQ=255)
                                                                                                                 cccGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTg                                   >  2:1191588/1‑129 (MQ=255)
                                                                                                                 cccGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTg                                   <  1:1191588/129‑1 (MQ=255)
                                                                                                                  ccGTCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAAtt                       <  2:1130102/140‑1 (MQ=255)
                                                                                                                     tCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTTCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCg                    <  2:1482935/140‑1 (MQ=255)
                                                                                                                     tCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCg                    <  1:614102/140‑1 (MQ=255)
                                                                                                                     tCCGCGCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCg                    <  1:1486139/140‑1 (MQ=255)
                                                                                                                       cgcgCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCg                    >  1:113012/1‑138 (MQ=255)
                                                                                                                       cgcgCCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCg                    <  2:113012/138‑1 (MQ=255)
                                                                                                                        gcgcCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCa                 <  2:1315435/140‑1 (MQ=255)
                                                                                                                        gcgcCAGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCa                 <  2:1112656/140‑1 (MQ=255)
                                                                                                                             aGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCaaa            <  1:937734/140‑1 (MQ=255)
                                                                                                                             aGTATTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGAGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCaaa            <  1:491888/140‑1 (MQ=255)
                                                                                                                                aTTGAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATAc         <  1:1121845/140‑1 (MQ=255)
                                                                                                                                   gAGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACggg      >  1:106431/1‑140 (MQ=255)
                                                                                                                                    aGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGt     >  2:1228753/1‑140 (MQ=255)
                                                                                                                                    aGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGt     <  2:81743/140‑1 (MQ=255)
                                                                                                                                    aGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGt     <  1:1228753/140‑1 (MQ=255)
                                                                                                                                    aGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGt     >  1:81743/1‑140 (MQ=255)
                                                                                                                                    aGCCACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGt     >  1:588990/1‑140 (MQ=255)
                                                                                                                                       cACCAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGaa  <  1:1442304/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                        
CCTGGAAGAGATTGCCTGGCGTAACGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAA  >  NC_000913/3974357‑3974631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A