Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 128 54.7 1845674 99.3% 1832754 139.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,765,794 (C)7→8 intergenic (‑18/‑124) yfjM ← / → rnlA CP4‑57 prophage; protein YfjM/CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,765,7871.C96.6% 209.2 / ‑0.1 59intergenic (‑11/‑131)yfjM/rnlACP4‑57 prophage; protein YfjM/CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base C (19/38);  total (21/38)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

GGCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATC  >  NC_000913/2765650‑2765911
                                                                                                                                           |                                                                                                                            
ggCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑cc                                                                                                                            >  1:538558/1‑140 (MQ=255)
     tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑ccccccc                                                                                                                       >  2:246563/1‑140 (MQ=255)
     tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑ccccccc                                                                                                                       <  1:914667/140‑1 (MQ=255)
     tctGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑ccccccc                                                                                                                       <  1:799304/140‑1 (MQ=255)
            gTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGa                                                                                                                 <  1:181534/140‑1 (MQ=255)
               cTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGTAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGa                                                                                                              >  1:481130/1‑140 (MQ=255)
                 gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATc                                                                                                            <  2:623521/140‑1 (MQ=255)
                 gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATc                                                                                                            <  1:184169/140‑1 (MQ=255)
                 gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATc                                                                                                            <  2:450692/140‑1 (MQ=255)
                 gTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATc                                                                                                            <  2:64573/140‑1 (MQ=255)
                    tCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTg                                                                                                         >  1:137817/1‑140 (MQ=255)
                    tCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTg                                                                                                         <  2:310017/140‑1 (MQ=255)
                     cGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGTAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGc                                                                                                        <  2:481130/140‑1 (MQ=255)
                       aCGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGccc                                                                                                      <  1:811794/140‑1 (MQ=255)
                        cGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCt                                                                                                     <  1:596700/140‑1 (MQ=255)
                         gTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCta                                                                                                    <  2:811878/140‑1 (MQ=255)
                         gTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCta                                                                                                    <  1:68042/140‑1 (MQ=255)
                                 ttttCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGa                                                                                            <  1:246563/140‑1 (MQ=255)
                                   ttCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGt                                                                                          <  2:595474/140‑1 (MQ=255)
                                   ttCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGt                                                                                          <  2:24177/140‑1 (MQ=255)
                                   ttCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGt                                                                                          <  1:606929/140‑1 (MQ=255)
                                    tCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTg                                                                                         <  1:713828/140‑1 (MQ=255)
                                      ttGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc                                                                                       <  1:275866/140‑1 (MQ=255)
                                      ttGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc                                                                                       <  1:539511/140‑1 (MQ=255)
                                      ttGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGcc                                                                                       <  1:574231/140‑1 (MQ=255)
                                       tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGccc                                                                                      >  2:662832/1‑140 (MQ=255)
                                       tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGccc                                                                                      >  2:103234/1‑140 (MQ=255)
                                             ctgGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCTTAtt                                                                                <  1:483323/140‑1 (MQ=255)
                                              tgGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTc                                                                               <  1:386493/140‑1 (MQ=255)
                                                     taatTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGc                                                                        >  1:859130/1‑140 (MQ=255)
                                                             aaGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAg                                                               >  1:912104/1‑140 (MQ=255)
                                                               gTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGAAGACGATCCTGCCCTATAGTGGGAGTGCCCCCTATTCAGCTGGCTAAATTTGGc                                                              >  2:624804/1‑140 (MQ=255)
                                                                tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAT‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCa                                                             >  2:640924/1‑140 (MQ=255)
                                                                tATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCa                                                             <  2:137817/140‑1 (MQ=255)
                                                                 aTCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCaaa                                                           >  2:75956/1‑140 (MQ=255)
                                                                      gTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGAACCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCaa                                                       >  2:318724/1‑140 (MQ=255)
                                                                        tgctgcTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                     <  1:851598/140‑1 (MQ=255)
                                                                        tgctgcTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                     <  1:25732/140‑1 (MQ=255)
                                                                          ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                   >  2:381801/1‑140 (MQ=255)
                                                                          ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                   >  2:349462/1‑140 (MQ=255)
                                                                          ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                   >  1:97030/1‑140 (MQ=255)
                                                                          ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                   >  2:298902/1‑140 (MQ=255)
                                                                          ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                   >  1:122290/1‑140 (MQ=255)
                                                                          ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                   >  1:845939/1‑140 (MQ=255)
                                                                          ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                   >  2:831368/1‑140 (MQ=255)
                                                                          ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                     >  1:27280/1‑138 (MQ=255)
                                                                          ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa                                                     <  2:27280/138‑1 (MQ=255)
                                                                               tGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCa                                              <  1:298902/140‑1 (MQ=255)
                                                                                ggTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                             <  1:745150/140‑1 (MQ=255)
                                                                                 gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgg                                            <  2:380950/140‑1 (MQ=255)
                                                                                  tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                           <  2:122290/140‑1 (MQ=255)
                                                                                  tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                           <  1:349462/140‑1 (MQ=255)
                                                                                  tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                           <  2:97030/140‑1 (MQ=255)
                                                                                       tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                      <  2:99792/140‑1 (MQ=255)
                                                                                       tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                      <  2:322858/140‑1 (MQ=255)
                                                                                        cAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTACAGGGGGGtat                                     <  2:730501/140‑1 (MQ=255)
                                                                                             agagGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTg                                <  1:640924/140‑1 (MQ=255)
                                                                                               agGTTACCGAAACGATGGACATCAACCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTa                              <  1:916300/140‑1 (MQ=255)
                                                                                                ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTag                             >  1:524010/1‑140 (MQ=255)
                                                                                                ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTag                             >  1:444014/1‑140 (MQ=255)
                                                                                                   tACCGAAACGATTGACAACCTTCCCCATTTTGTTGACTTC‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGt                          <  2:476221/140‑1 (MQ=255)
                                                                                                   tACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGt                          >  2:440325/1‑140 (MQ=255)
                                                                                                                      atcCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                      >  2:523082/1‑125 (MQ=255)
                                                                                                                      atcCCCCATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                      <  1:523082/125‑1 (MQ=255)
                                                                                                                           ccATTTTGTGGAC‑TGCCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATc  <  2:859130/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                            
GGCTCTCTGCTCGTTCTGTTTCGACGTAGAATTTTTTCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGAC‑TG‑CCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATC  >  NC_000913/2765650‑2765911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A