Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 128 54.7 1845674 99.3% 1832754 139.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,811,1340AG100.0% 208.0 / NA 57H122R (CAC→CGC) mprADNA‑binding transcriptional repressor MprA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/32);  total (25/32)

TGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAA  >  NC_000913/2811006‑2811270
                                                                                                                                |                                                                                                                                        
tgCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgc                                                                                                                               >  2:305050/1‑140 (MQ=255)
 gCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgcg                                                                                                                              <  2:890754/140‑1 (MQ=255)
  ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                             <  1:111672/140‑1 (MQ=255)
  ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                             <  2:883719/140‑1 (MQ=255)
     ttGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAg                                                                                                                          >  2:250160/1‑140 (MQ=255)
     ttGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAg                                                                                                                          >  1:800462/1‑140 (MQ=255)
     ttGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAg                                                                                                                          >  1:762310/1‑140 (MQ=255)
           catcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTa                                                                                                                    >  2:596573/1‑140 (MQ=255)
             tcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGa                                                                                                                           >  2:617474/1‑131 (MQ=255)
             tcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGa                                                                                                                           <  1:617474/131‑1 (MQ=255)
             tcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTacc                                                                                                                  >  2:752667/1‑140 (MQ=255)
             tcCCATACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTacc                                                                                                                  >  1:282953/1‑140 (MQ=255)
              cccGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTacca                                                                                                                 >  1:752416/1‑140 (MQ=255)
               ccGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTaccac                                                                                                                <  1:327161/140‑1 (MQ=255)
               ccGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTaccac                                                                                                                <  2:868141/140‑1 (MQ=255)
                cGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTaccacc                                                                                                               <  2:873684/140‑1 (MQ=255)
                  tACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCgc                                                                                                             >  1:594222/1‑140 (MQ=255)
                   aCCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCgca                                                                                                            >  2:486367/1‑140 (MQ=255)
                   aCCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCgca                                                                                                            <  1:917056/140‑1 (MQ=255)
                       acgcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCAt                                                                                                        >  1:749598/1‑140 (MQ=255)
                        cgcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATa                                                                                                       >  2:411784/1‑140 (MQ=255)
                        cgcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATa                                                                                                       >  1:783649/1‑140 (MQ=255)
                        cgcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATa                                                                                                       >  1:561858/1‑140 (MQ=255)
                         gcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATaa                                                                                                      <  2:783649/140‑1 (MQ=255)
                              gcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActgcc                                                                                                 <  1:601287/140‑1 (MQ=255)
                              gcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActgcc                                                                                                 <  2:740089/140‑1 (MQ=255)
                                   ttGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCAt                                                                                            <  2:750883/140‑1 (MQ=255)
                                   ttGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCAt                                                                                            >  2:376981/1‑140 (MQ=255)
                                   ttGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCAt                                                                                            <  1:486367/140‑1 (MQ=255)
                                           gAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTg                                                                                    <  2:307362/140‑1 (MQ=255)
                                           gAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTg                                                                                    >  2:787520/1‑140 (MQ=255)
                                           gAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTg                                                                                    >  1:699/1‑140 (MQ=255)
                                               tGGAAAAACGCGCTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTcc                                                                                <  1:596573/140‑1 (MQ=255)
                                                  aaaaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCcgcg                                                                             <  2:594222/140‑1 (MQ=255)
                                                       cgcgGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAg                                                                        >  2:484744/1‑140 (MQ=255)
                                                       cgcgGTTGGATCGAACGTCGTGAAAGCGAAAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAg                                                                        >  2:184326/1‑140 (MQ=255)
                                                         cgGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGca                                                                      <  1:163362/140‑1 (MQ=255)
                                                           gTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGcaca                                                                    <  1:305050/140‑1 (MQ=255)
                                                            ttGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCacaa                                                                   >  2:641850/1‑140 (MQ=255)
                                                                 tCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGaa                                                              <  2:699/140‑1 (MQ=255)
                                                                      cgtcgtGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAGCGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTACCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGa                                                         >  2:634312/1‑140 (MQ=255)
                                                                           tGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGc                                                    <  2:572763/140‑1 (MQ=255)
                                                                           tGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGc                                                    <  1:250160/140‑1 (MQ=255)
                                                                           tGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGc                                                    >  2:601573/1‑140 (MQ=255)
                                                                              aaGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCg                                                 >  1:516866/1‑140 (MQ=255)
                                                                                     aaCGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAAt                                          <  1:787520/140‑1 (MQ=255)
                                                                                          tCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCAccc                                     <  1:494181/140‑1 (MQ=255)
                                                                                             ccgcTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCa                                  <  1:891268/140‑1 (MQ=255)
                                                                                             ccgcTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCa                                  <  2:762310/140‑1 (MQ=255)
                                                                                             ccgcTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCa                                  <  2:800462/140‑1 (MQ=255)
                                                                                                 tgcctgcATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAAtt                              >  2:788220/1‑140 (MQ=255)
                                                                                                  gcctgcATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTg                             <  1:752667/140‑1 (MQ=255)
                                                                                                   cctgcATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGc                            >  2:559930/1‑140 (MQ=255)
                                                                                                    ctgcATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGct                           <  2:184851/140‑1 (MQ=255)
                                                                                                         tCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTccc                      <  2:752416/140‑1 (MQ=255)
                                                                                                                 tAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGAc              <  1:641850/140‑1 (MQ=255)
                                                                                                                    cGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAg           >  2:563893/1‑140 (MQ=255)
                                                                                                                     ggAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGa          <  1:673825/140‑1 (MQ=255)
                                                                                                                             gTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGTTCTCCCGTCTCGACCAGATGGAACaa  <  2:282953/140‑1 (MQ=255)
                                                                                                                                |                                                                                                                                        
TGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAACAA  >  NC_000913/2811006‑2811270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A