Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	128	54.7	1845674	99.3%	1832754	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,292,906	A→G	A465A (GCA→GCG)	nrfE →	putative cytochrome c‑type biogenesis protein NrfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,292,906	0	A	G	100.0%	187.4 / NA	51	A465A (GCA→GCG)	nrfE	putative cytochrome c‑type biogenesis protein NrfE
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (32/19); total (32/19)

CAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAA  >  NC_000913/4292771‑4293042

cAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGt                                                                                                                                      >  1:781580/1‑140 (MQ=255)

   cGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTg                                                                                                                                   <  2:850885/140‑1 (MQ=255)

     tCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGAc                                                                                                                                 >  1:43984/1‑140 (MQ=255)

        gCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTg                                                                                                                                   >  2:486281/1‑135 (MQ=255)

        gCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTg                                                                                                                                   <  1:486281/135‑1 (MQ=255)

              aGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGtt                                                                                                                                     >  2:285192/1‑127 (MQ=255)

              aGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGtt                                                                                                                                     <  1:285192/127‑1 (MQ=255)

                gagaTCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACg                                                                                                                      <  1:519209/140‑1 (MQ=255)

                gagaTCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACg                                                                                                                      <  1:454153/140‑1 (MQ=255)

                        ccTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTg                                                                                                              >  1:719551/1‑140 (MQ=255)

                        ccTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTg                                                                                                              >  2:670354/1‑140 (MQ=255)

                        ccTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTg                                                                                                              >  1:353908/1‑140 (MQ=255)

                                 aCAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGc                                                                                                     >  1:736930/1‑140 (MQ=255)

                                      cGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAg                                                                                                <  1:395191/140‑1 (MQ=255)

                                                          gCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACg                                                                            <  1:673252/140‑1 (MQ=255)

                                                           cAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGc                                                                           >  1:415777/1‑140 (MQ=255)

                                                                        ccGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGcc                                                                          <  2:191413/128‑1 (MQ=255)

                                                                        ccGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGcc                                                                          >  1:191413/1‑128 (MQ=255)

                                                                        ccGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAatga                                                              >  1:278196/1‑140 (MQ=255)

                                                                        ccGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAatga                                                              >  2:134327/1‑140 (MQ=255)

                                                                         cGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTc                                                                       >  2:75550/1‑130 (MQ=255)

                                                                         cGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTc                                                                       <  1:75550/130‑1 (MQ=255)

                                                                         cGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAatgat                                                             >  2:744509/1‑140 (MQ=255)

                                                                                  tGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTc                                                    >  1:52161/1‑140 (MQ=255)

                                                                                     cTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGGACTGACGCCAGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAAt                                                 >  2:825544/1‑140 (MQ=255)

                                                                                         aTCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCAGTCAACAAATGATGGAACCGTCAATTCGc                                             <  2:415777/140‑1 (MQ=255)

                                                                                          tCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCt                                            >  2:167925/1‑140 (MQ=255)

                                                                                              cAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGaa                                        >  2:844765/1‑140 (MQ=255)

                                                                                              cAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGaa                                        >  2:2689/1‑140 (MQ=255)

                                                                                                aGCCAAAGGCAATTGCACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACg                                      <  1:670354/140‑1 (MQ=255)

                                                                                                   cAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCa                                   >  1:849583/1‑140 (MQ=255)

                                                                                                    aaaGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCAt                                  >  2:448905/1‑140 (MQ=255)

                                                                                                    aaaGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCAt                                  >  1:96797/1‑140 (MQ=255)

                                                                                                    aaaGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCAt                                  >  1:889982/1‑140 (MQ=255)

                                                                                                      aGGCAATTACACGAGGGAAAAAGCGATAGAGGCGCTGTATGACCATCAGCAACGCATTGGTGGACTGTCGCCGGAGCGGCGTTTTTATGATGTACGCCGTCAACAAATGATGGAACCGTCAATTCTCTGGTACGGCATcc                                >  2:496840/1‑140 (MQ=255)

                                                                                                      aGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATcc                                >  2:651321/1‑140 (MQ=255)

                                                                                                      aGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATcc                                >  2:873872/1‑140 (MQ=255)

                                                                                                      aGGCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATcc                                >  1:542411/1‑140 (MQ=255)

                                                                                                       ggCAATTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCa                               <  1:167925/140‑1 (MQ=255)

                                                                                                          aaTTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGa                            <  2:52161/140‑1 (MQ=255)

                                                                                                           aTTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGAc                           <  2:353908/140‑1 (MQ=255)

                                                                                                           aTTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGAc                           <  2:719551/140‑1 (MQ=255)

                                                                                                           aTTACACCAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGAc                           >  1:119568/1‑140 (MQ=255)

                                                                                                                 ccAGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTAt                     >  2:66190/1‑140 (MQ=255)

                                                                                                                   aGCGAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGc                   <  1:385872/140‑1 (MQ=255)

                                                                                                                      gAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGt                >  2:264448/1‑140 (MQ=255)

                                                                                                                      gAAAAAGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGt                >  2:133091/1‑140 (MQ=255)

                                                                                                                       aaaaaGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTc               >  2:16486/1‑140 (MQ=255)

                                                                                                                        aaaaGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCa              <  2:710911/140‑1 (MQ=255)

                                                                                                                          aaGCGATAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATg            <  1:264448/140‑1 (MQ=255)

                                                                                                                               aTAGTGGCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGga       >  2:235496/1‑140 (MQ=255)

                                                                                                                                    ggCGCTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGaaaa  <  2:849583/140‑1 (MQ=255)

CAGCGTCAGCCGTCAGGAGATCAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGTGCCTCGATCTACAAGCCAAAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGTTTGACCATCAGCAACGCATTGGTGAACTGACGCCGGAGCGGCGTTTTTATGAAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAA  >  NC_000913/4292771‑4293042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A