Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	116	72.9	2471836	99.3%	2454533	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,910,454	G→A	R78C (CGC→TGC)	mazG ←	nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,910,454	0	G	A	98.5%	227.3 / ‑3.8	68	coding (232/792 nt)	mazG	nucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (37/30); minor base . (1/0); total (38/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

GCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCG  >  NC_000913/2910319‑2910588

gcTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCct                                                                                                                                    <  2:937156/140‑1 (MQ=255)

                  gCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTgc                                                                                                                  <  1:506707/140‑1 (MQ=255)

                  gCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTgc                                                                                                                  <  2:586892/140‑1 (MQ=255)

                  gCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTgc                                                                                                                  <  2:335167/140‑1 (MQ=255)

                  gCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTgc                                                                                                                  <  2:1126639/140‑1 (MQ=255)

                   cTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTgcg                                                                                                                 >  2:1159832/1‑140 (MQ=255)

                   cTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTgcg                                                                                                                 >  2:836369/1‑140 (MQ=255)

                     cccAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGcgcg                                                                                                               <  1:686774/140‑1 (MQ=255)

                        aaCGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAACAACATGCGGATGGCGACGCTCTAATTTATCGCTAATATCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTaa                                                                                                            >  2:681704/1‑140 (MQ=255)

                        aaCGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTaa                                                                                                            >  1:425422/1‑140 (MQ=255)

                               aaGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAAcacca                                                                                                     <  2:1164876/140‑1 (MQ=255)

                                aGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAAcaccac                                                                                                    >  1:1211687/1‑140 (MQ=255)

                                aGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAAcacca                                                                                                     <  2:718397/139‑1 (MQ=255)

                                aGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAAcacca                                                                                                     >  1:718397/1‑139 (MQ=255)

                                 gCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACaccacc                                                                                                   <  2:896012/140‑1 (MQ=255)

                                 gCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACaccacc                                                                                                   <  2:1211687/140‑1 (MQ=255)

                                 gCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACaccacc                                                                                                   <  1:1159832/140‑1 (MQ=255)

                                   aCTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTg                                                                                                 >  2:108853/1‑140 (MQ=255)

                                       cACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACaccacc                                                                                                   >  2:1128606/1‑134 (MQ=255)

                                       cACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACaccacc                                                                                                   <  1:1128606/134‑1 (MQ=255)

                                       cACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAAt                                                                                             >  1:15415/1‑140 (MQ=255)

                                       cACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAAt                                                                                             >  2:193298/1‑140 (MQ=255)

                                           actGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCa                                                                                         <  2:389600/140‑1 (MQ=255)

                                           actGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCa                                                                                         >  1:541967/1‑140 (MQ=255)

                                            ctGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAg                                                                                        >  1:131621/1‑140 (MQ=255)

                                             tGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGa                                                                                       <  1:822626/140‑1 (MQ=255)

                                               ttttCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACTCCACCTGGAATAGCAGATc                                                                                     >  1:218783/1‑140 (MQ=255)

                                               ttttCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGa                                                                                       >  1:282216/1‑138 (MQ=255)

                                               ttttCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGa                                                                                       <  2:282216/138‑1 (MQ=255)

                                               ttttCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATc                                                                                     >  1:657765/1‑140 (MQ=255)

                                               ttttCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATc                                                                                     >  1:1164435/1‑140 (MQ=255)

                                               ttttCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATc                                                                                     >  1:800646/1‑140 (MQ=255)

                                                      cAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGt                                                                              >  1:302747/1‑140 (MQ=255)

                                                         aaCTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCa                                                                           >  1:966890/1‑140 (MQ=255)

                                                              tCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACTCCACCTGGAATAGCAGATCGCCCAGTTCACcgcg                                                                      <  2:218783/140‑1 (MQ=255)

                                                              tCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACcgcg                                                                      >  2:452448/1‑140 (MQ=255)

                                                                   aaaaaCATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGAt                                                                 >  1:224882/1‑140 (MQ=255)

                                                                      aaCATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGt                                                              <  2:225776/140‑1 (MQ=255)

                                                                      aaCATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGGTCACCGCGAAGATCGt                                                              >  2:1106081/1‑140 (MQ=255)

                                                                       aCATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTc                                                             <  2:966890/140‑1 (MQ=255)

                                                                          tGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGt                                                              >  2:886464/1‑136 (MQ=255)

                                                                          tGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGt                                                              <  1:886464/136‑1 (MQ=255)

                                                                                tGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTc                                                    >  1:367957/1‑140 (MQ=255)

                                                                                 ggCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCa                                                   <  2:367957/140‑1 (MQ=255)

                                                                                  gCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCAc                                                  >  1:228767/1‑140 (MQ=255)

                                                                                          ctAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATg                                          <  1:836369/140‑1 (MQ=255)

                                                                                                tATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTcc                                    >  1:645244/1‑140 (MQ=255)

                                                                                                tATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTcc                                    >  1:146685/1‑140 (MQ=255)

                                                                                                 aTCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCa                                                   <  1:658182/124‑1 (MQ=255)

                                                                                                 aTCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCa                                                   >  2:658182/1‑124 (MQ=255)

                                                                                                  tCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAg                                  <  1:108853/140‑1 (MQ=255)

                                                                                                    gCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCa                                <  2:1205055/140‑1 (MQ=255)

                                                                                                     cTAATAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCAc                               >  2:26502/1‑140 (MQ=255)

                                                                                                         tAGCAGCGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCg                           <  2:541967/140‑1 (MQ=255)

                                                                                                            cagcGCAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAg                        <  2:800646/140‑1 (MQ=255)

                                                                                                               cgcAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGtt                     >  2:746669/1‑140 (MQ=255)

                                                                                                                gcAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGttt                    >  2:304339/1‑140 (MQ=255)

                                                                                                                gcAAATATCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGttt                    >  2:824159/1‑140 (MQ=255)

                                                                                                                gcAAATATCATTAAAGTCAAAGC‑ACCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTttc                   >  2:486696/1‑140 (MQ=255)

                                                                                                                     tatCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCa               <  1:824159/140‑1 (MQ=255)

                                                                                                                     tatCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCa               <  2:1059107/140‑1 (MQ=255)

                                                                                                                     tatCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCa               <  2:228767/140‑1 (MQ=255)

                                                                                                                     tatCATTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCa               <  2:425422/140‑1 (MQ=255)

                                                                                                                         aTTAAAGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAggg           >  1:256779/1‑140 (MQ=255)

                                                                                                                              aGTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTaa      >  1:1213070/1‑140 (MQ=255)

                                                                                                                               gTCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAg     >  2:13719/1‑140 (MQ=255)

                                                                                                                                tCAAAGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAgg    >  1:956089/1‑140 (MQ=255)

                                                                                                                                  aaaGCACCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGgcg  >  1:224118/1‑140 (MQ=255)

GCTCTTCGGTTTTGATTTGCTCCCAACGGGCAAGCACTTCACTACTGTTTTCGGCAGAACTATCAGCAAAAACATGCGGATGGCGACGCTCTAATTTATCGCTAATAGCAGCGCAAATATCATTAAAGTCAAAGCGCCCTTCTTCCTGAGCCATTTGCGCGTAAAACACCACCTGGAATAGCAGATCGCCCAGTTCACCGCGAAGATCGTCAAAATCTTCACGGGCGATGGCGTCCAGCACTTCGTAGGTTTCTTCAAGGGTGTAAGGCG  >  NC_000913/2910319‑2910588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A