Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	116	72.9	2471836	99.3%	2454533	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,020,743	A→G	A68A (GCA→GCG)	ygfM →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,020,743	0	A	G	100.0%	283.5 / NA	78	A68A (GCA→GCG)	ygfM	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (45/33); total (45/33)

AGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGT  >  NC_000913/3020611‑3020879

aGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGtctc                                                                                                                                   >  2:488128/1‑140 (MQ=255)

aGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGtctc                                                                                                                                   >  2:657442/1‑140 (MQ=255)

     tCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTg                                                                                                                              >  1:94041/1‑140 (MQ=255)

     tCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTg                                                                                                                              >  2:104301/1‑140 (MQ=255)

     tCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTg                                                                                                                              >  1:58353/1‑140 (MQ=255)

     tCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTg                                                                                                                              <  1:488128/140‑1 (MQ=255)

     tCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTg                                                                                                                              >  1:395617/1‑140 (MQ=255)

        ggTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAg                                                                                                                           <  1:271281/140‑1 (MQ=255)

               cGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGc                                                                                                                    >  1:611516/1‑140 (MQ=255)

                   ggCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGa                                                                                                                >  1:859825/1‑140 (MQ=255)

                             tCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGAttt                                                                                                      <  2:934303/140‑1 (MQ=255)

                               aaTGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTAt                                                                                                    <  2:611516/140‑1 (MQ=255)

                                    tACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTg                                                                                               <  1:104301/140‑1 (MQ=255)

                                    tACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTg                                                                                               <  1:289580/140‑1 (MQ=255)

                                     acacCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATgcg                                                                                                            >  2:606195/1‑126 (MQ=255)

                                     acacCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATgcg                                                                                                            <  1:606195/126‑1 (MQ=255)

                                       acCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAg                                                                                            >  1:55186/1‑140 (MQ=255)

                                       acCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAg                                                                                            >  2:631876/1‑140 (MQ=255)

                                           aCCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAgcgc                                                                                         <  2:794129/139‑1 (MQ=255)

                                           aCCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAgcgc                                                                                         >  1:794129/1‑139 (MQ=255)

                                           aCCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCt                                                                                        >  1:566001/1‑140 (MQ=255)

                                           aCCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCt                                                                                        >  1:1221952/1‑140 (MQ=255)

                                              cGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCa                                                                                             <  2:1155823/132‑1 (MQ=255)

                                              cGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCa                                                                                             >  1:1155823/1‑132 (MQ=255)

                                                tACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTg                                                                                   >  1:439665/1‑140 (MQ=255)

                                                tACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTg                                                                                   >  2:387907/1‑140 (MQ=255)

                                                tACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTg                                                                                   <  2:859825/140‑1 (MQ=255)

                                                  ccGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGaa                                                                                 <  2:869745/140‑1 (MQ=255)

                                                     aTAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGcc                                                                              >  2:830181/1‑140 (MQ=255)

                                                         aaaGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCg                                                                          <  2:439665/140‑1 (MQ=255)

                                                           aGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGt                                                                        <  2:98008/140‑1 (MQ=255)

                                                           aGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGt                                                                        <  1:657442/140‑1 (MQ=255)

                                                                    tttCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTAc                                                               >  1:415098/1‑140 (MQ=255)

                                                                       ccTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTATTCa                                                            >  1:323256/1‑140 (MQ=255)

                                                                       ccTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACt                                                              >  1:503533/1‑138 (MQ=255)

                                                                       ccTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACt                                                              <  2:503533/138‑1 (MQ=255)

                                                                       ccTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCa                                                            <  2:55186/140‑1 (MQ=255)

                                                                            cAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCa                                                       <  1:65470/140‑1 (MQ=255)

                                                                            cAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCa                                                       <  1:631876/140‑1 (MQ=255)

                                                                            cAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCa                                                       <  2:1221952/140‑1 (MQ=255)

                                                                            cAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCa                                                       <  2:1161737/140‑1 (MQ=255)

                                                                              ggATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATg                                                     >  2:810450/1‑140 (MQ=255)

                                                                               gATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACt                                                              >  2:514897/1‑130 (MQ=255)

                                                                               gATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACt                                                              <  1:514897/130‑1 (MQ=255)

                                                                               gATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGt                                                    >  1:897407/1‑140 (MQ=255)

                                                                                 tCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTTGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTc                                                  >  2:90626/1‑140 (MQ=255)

                                                                                 tCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTc                                                  >  2:38030/1‑140 (MQ=255)

                                                                                 tCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTc                                                  <  2:869055/140‑1 (MQ=255)

                                                                                 tCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTc                                                  >  1:869055/1‑140 (MQ=255)

                                                                                    ggAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTGCTCACGCCATGTTCGTa                                               >  2:1097016/1‑140 (MQ=255)

                                                                                    ggAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTa                                               <  1:387907/140‑1 (MQ=255)

                                                                                           gactggGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTc                                        >  1:718663/1‑140 (MQ=255)

                                                                                           gactggGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTc                                        >  1:1117935/1‑140 (MQ=255)

                                                                                           gactggGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTc                                        >  1:674729/1‑140 (MQ=255)

                                                                                           gactggGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTc                                        >  2:436190/1‑140 (MQ=255)

                                                                                           gactggGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTc                                        >  2:294783/1‑140 (MQ=255)

                                                                                           gactggGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTc                                        >  1:640467/1‑140 (MQ=255)

                                                                                            actggGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCg                                       >  2:49886/1‑140 (MQ=255)

                                                                                              tggGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGAc                                     <  1:183398/140‑1 (MQ=255)

                                                                                                 gTTGACTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCAt                                  <  2:718663/140‑1 (MQ=255)

                                                                                                     aCTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAAt                                             >  2:237490/1‑125 (MQ=255)

                                                                                                     aCTGGGATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAAt                                             <  1:237490/125‑1 (MQ=255)

                                                                                                          gATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAg                                          <  2:251940/123‑1 (MQ=255)

                                                                                                          gATAACGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAg                                          >  1:251940/1‑123 (MQ=255)

                                                                                                             aaCGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAAt                      <  2:288711/140‑1 (MQ=255)

                                                                                                               cGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGTCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATcg                    >  1:290158/1‑140 (MQ=255)

                                                                                                               cGGTGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATcg                    >  2:887201/1‑140 (MQ=255)

                                                                                                                  tGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATcgccg                 <  2:566001/140‑1 (MQ=255)

                                                                                                                  tGCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATcgccg                 <  2:897407/140‑1 (MQ=255)

                                                                                                                   gCACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATcgccgc                <  1:1122194/140‑1 (MQ=255)

                                                                                                                    cACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCgccgcc               >  1:1215634/1‑140 (MQ=255)

                                                                                                                    cACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCgccgcc               >  2:681948/1‑140 (MQ=255)

                                                                                                                    cACTGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTAAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCgccgcc               >  2:857429/1‑140 (MQ=255)

                                                                                                                       tGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGccgcccgc            >  1:317319/1‑140 (MQ=255)

                                                                                                                       tGCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGccgcccgc            <  2:317319/140‑1 (MQ=255)

                                                                                                                        gCGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCcgcccgcc           <  1:38030/140‑1 (MQ=255)

                                                                                                                         cGGATTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCa          >  2:620308/1‑140 (MQ=255)

                                                                                                                            aTTGGCGCGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGa       <  2:1215634/140‑1 (MQ=255)

                                                                                                                                gcgcGATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAagag   >  2:342053/1‑140 (MQ=255)

                                                                                                                                 cgcgATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGt  <  2:640467/140‑1 (MQ=255)

AGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAACTGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTTATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAATCGCCGCCCGCCAGGAAGAGT  >  NC_000913/3020611‑3020879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A