Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	116	72.9	2471836	99.3%	2454533	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,592,948	G→C	G431A (GGC→GCC)	tsr →	methyl‑accepting chemotaxis protein Tsr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,592,948	0	G	C	100.0%	173.3 / NA	49	G431A (GGC→GCC)	tsr	methyl‑accepting chemotaxis protein Tsr
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (26/23); total (26/23)

CGCGTGCGGGTGAGCAAGGGCGCGGTTTTGCGGTGGTCGCGGGAGAAGTGCGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCG  >  NC_000913/4592816‑4593084

cgcgTGCGGGTGAGCAAGGGCGCGGTTTTGCGGTGGTCGCGGGAGAAGTGCGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACg                                                                                                                                   <  1:1157940/140‑1 (MQ=255)

cgcgTGCGGGTGAGCAAGGGCGCGGTTTTGCGGTGGTCGCGGGAGAAGTGCGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACg                                                                                                                                   <  1:1034025/140‑1 (MQ=255)

         gTGAGCAAGGGCGCGGTTTTGCGGTGGTCGCGGGAGAAGTGCGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGaa                                                                                                                          <  2:433351/140‑1 (MQ=255)

            aGCAAGGGCGCGGTTTTGCGG‑GGTCGCGGGAGAAGTGCGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAgcg                                                                                                                      <  2:477052/140‑1 (MQ=255)

                  ggCGCGGTTTTGCGGTGGTCGCGGGAGAAGTGCGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCCGGTCGAAAGCGCCggg                                                                                                                 <  2:320960/140‑1 (MQ=255)

                      cgGTTTTGCGGTGGTCGCGGGAGAAGTGCGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGaaa                                                                                                             >  2:690954/1‑140 (MQ=255)

                                   gtCGCGGGAGAAGTGCGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGAt                                                                                                >  2:762762/1‑140 (MQ=255)

                                   gtCGCGGGAGAAGTGCGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGAt                                                                                                >  2:611123/1‑140 (MQ=255)

                                   gtCGCGGGAGAAGTGCGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGAt                                                                                                <  1:1182649/140‑1 (MQ=255)

                                   gtCGCGGGAGAAGTGCGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGAt                                                                                                <  1:690954/140‑1 (MQ=255)

                                   gtCGCGGGAGAAGTGCGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGAt                                                                                                >  1:739753/1‑140 (MQ=255)

                                     cgcgGGAGAAGTGCGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTg                                                                                              >  2:1231212/1‑140 (MQ=255)

                                                    tAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGAccc                                                                               <  2:805725/140‑1 (MQ=255)

                                                          ggCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGa                                                                         <  2:739753/140‑1 (MQ=255)

                                                          ggCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGa                                                                         <  1:762762/140‑1 (MQ=255)

                                                           gCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGAc                                                                        >  2:544008/1‑140 (MQ=255)

                                                            cccAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACg                                                                       >  2:217880/1‑140 (MQ=255)

                                                               agcgcagcgcCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGAc                                                                    >  2:355939/1‑140 (MQ=255)

                                                               agcgcagcgcCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGAc                                                                    >  1:370231/1‑140 (MQ=255)

                                                                 cgcagcgcCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACAt                                                                  >  1:714013/1‑140 (MQ=255)

                                                                 cgcagcgcCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACAt                                                                  >  1:216566/1‑140 (MQ=255)

                                                                 cgcagcgcCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACAt                                                                  >  1:385090/1‑140 (MQ=255)

                                                                 cgcagcgcCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACAt                                                                  >  2:512790/1‑140 (MQ=255)

                                                                 cgcagcgcCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACAt                                                                  >  2:652750/1‑140 (MQ=255)

                                                                     gcgcCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATg                                                              >  2:692890/1‑140 (MQ=255)

                                                                        cccAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGc                                                           <  1:286480/140‑1 (MQ=255)

                                                                                 cTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATtgct                                                  <  2:134377/140‑1 (MQ=255)

                                                                                   cGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATtgcttc                                                <  2:385090/140‑1 (MQ=255)

                                                                                       aaaTTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATtgcttctgct                                            >  1:1187982/1‑140 (MQ=255)

                                                                                           tAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGACTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGcttctgcttctg                                        <  2:919425/140‑1 (MQ=255)

                                                                                             aaaGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCtgat                                      <  2:1207995/140‑1 (MQ=255)

                                                                                               aGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCtgatga                                    >  2:810489/1‑140 (MQ=255)

                                                                                                     aTTGAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCagag                              >  2:547426/1‑140 (MQ=255)

                                                                                                        gAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCg                           <  1:355939/140‑1 (MQ=255)

                                                                                                        gAAGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCg                           <  2:640351/140‑1 (MQ=255)

                                                                                                         aaGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGt                          >  2:968598/1‑140 (MQ=255)

                                                                                                          aGACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTg                         <  1:217880/140‑1 (MQ=255)

                                                                                                           gACTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTgg                        <  2:370231/140‑1 (MQ=255)

                                                                                                            aCTCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGt                       <  2:590517/140‑1 (MQ=255)

                                                                                                              tCGGTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGATATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTAt                     >  2:849556/1‑140 (MQ=255)

                                                                                                               cGGTGGGGAGAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATc                    >  2:534601/1‑140 (MQ=255)

                                                                                                                ggTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCg                   >  2:99845/1‑140 (MQ=255)

                                                                                                                ggTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCg                   >  2:97864/1‑140 (MQ=255)

                                                                                                                ggTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCg                   >  1:28300/1‑140 (MQ=255)

                                                                                                                 gTGGGGAAAGTGGATGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGa                  <  1:836777/140‑1 (MQ=255)

                                                                                                                               tGTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAg    >  2:545517/1‑140 (MQ=255)

                                                                                                                                gTTGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGc   >  1:632560/1‑140 (MQ=255)

                                                                                                                                cttGCCTCTACGCTGGTCGAAAGCGCGGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGc   >  2:202365/2‑140 (MQ=255)

                                                                                                                                 ttGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCg  <  1:692890/140‑1 (MQ=255)

                                                                                                                                 ttGCCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCg  <  1:512790/140‑1 (MQ=255)

CGCGTGCGGGTGAGCAAGGGCGCGGTTTTGCGGTGGTCGCGGGAGAAGTGCGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCG  >  NC_000913/4592816‑4593084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A