Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 121 68.4 2351762 99.3% 2335299 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,905,639 (A)9→8 intergenic (‑42/+92) bglF ← / ← bglG beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase/transcriptional antiterminator BglG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,905,6310A.100.0% 539.6 / NA 127intergenic (‑34/+100)bglF/bglGbeta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase/transcriptional antiterminator BglG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (50/77);  total (50/77)

Only 100 of 130 total aligned reads displayed.

CATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCCAAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATAT  >  NC_000913/3905495‑3905759
                                                                                                                                        |                                                                                                                                 
cATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCCaaaa                                                                                                                                >  1:637761/1‑140 (MQ=255)
      ttaatttaaAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCCaaaaaaaacc                                                                                                                          >  2:890086/1‑138 (MQ=255)
         atttaaAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCG‑CCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGaa                                                                                                                     >  2:474751/1‑140 (MQ=255)
             aaaaCGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGaaaaa                                                                                                                  >  2:164968/1‑140 (MQ=255)
             aaaaCGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGaaaaa                                                                                                                  >  2:289994/1‑140 (MQ=255)
               aaCGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGaaaaaaa                                                                                                                >  1:322178/1‑140 (MQ=255)
                   tAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGc                                                                                                            <  2:698680/140‑1 (MQ=255)
                   tAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGc                                                                                                            <  1:855339/140‑1 (MQ=255)
                    aaTCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGcc                                                                                                           >  1:396876/1‑140 (MQ=255)
                    aaTCGCGTTGCGCAATGCATCAGACTCACAATGTTACCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGcc                                                                                                           <  1:1138992/140‑1 (MQ=255)
                                        cAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAATAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑ttt                                                                                      >  1:1043443/1‑140 (MQ=255)
                                        cAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑ttt                                                                                      >  2:1173994/1‑140 (MQ=255)
                                        cAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑ttt                                                                                      >  1:733581/1‑140 (MQ=255)
                                        cAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑ttt                                                                                      >  1:797382/1‑140 (MQ=255)
                                               acaATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAg                                                                               >  1:292713/1‑140 (MQ=255)
                                               acaATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAg                                                                               >  1:3236/1‑140 (MQ=255)
                                                     ttATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTg                                                                         <  2:322178/140‑1 (MQ=255)
                                                       aTCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGcc                                                                       >  1:439360/1‑140 (MQ=255)
                                                           gcgcCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCt                                                                   >  1:276398/1‑140 (MQ=255)
                                                           gcgcCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCt                                                                   >  1:109564/1‑140 (MQ=255)
                                                           gcgcCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCt                                                                   >  2:303301/1‑140 (MQ=255)
                                                           gcgcCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCt                                                                   >  1:701344/1‑140 (MQ=255)
                                                            cgcCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCtt                                                                  <  2:947266/140‑1 (MQ=255)
                                                             gcCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTa                                                                 >  2:191771/1‑140 (MQ=255)
                                                             gcCCCCGACTCCTGCGACTATTTTTCTGACTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTa                                                                 >  1:498897/1‑140 (MQ=255)
                                                              cccccGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAg                                                                <  1:483933/140‑1 (MQ=255)
                                                               ccccGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGt                                                               >  1:765403/1‑140 (MQ=255)
                                                               ccccGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGt                                                               >  2:1067740/1‑140 (MQ=255)
                                                                 ccGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGc                                                             <  2:136114/140‑1 (MQ=255)
                                                                 ccGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGc                                                             <  2:439360/140‑1 (MQ=255)
                                                                   gACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCgg                                                           <  1:541557/140‑1 (MQ=255)
                                                                    aCTCCTGCGACTA‑TTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑‑AAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTaa                                                        <  1:178714/140‑1 (MQ=255)
                                                                      tCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTaa                                                        <  1:4310/140‑1 (MQ=255)
                                                                      tCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTaa                                                        <  1:74204/140‑1 (MQ=255)
                                                                      tCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTaa                                                        <  1:376358/140‑1 (MQ=255)
                                                                      tCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTaa                                                        >  1:133324/1‑140 (MQ=255)
                                                                        cTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAATAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACa                                                      <  2:1043443/140‑1 (MQ=255)
                                                                        cTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACa                                                      <  1:1023330/140‑1 (MQ=255)
                                                                        cTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACAGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACa                                                      >  2:91492/1‑140 (MQ=255)
                                                                         tGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTaa                                                        <  2:329739/137‑1 (MQ=255)
                                                                         tGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTaa                                                        >  1:329739/1‑137 (MQ=255)
                                                                         tGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACaa                                                     >  1:496950/1‑140 (MQ=255)
                                                                         tGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACaa                                                     >  2:831389/1‑140 (MQ=255)
                                                                                aTTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACa                                                      >  1:921249/1‑132 (MQ=255)
                                                                                aTTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACa                                                      <  2:921249/132‑1 (MQ=255)
                                                                                aTTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTa                                              <  2:257076/140‑1 (MQ=255)
                                                                                 tttttCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTAc                                             <  2:529141/140‑1 (MQ=255)
                                                                                  ttttCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACt                                            <  1:978758/140‑1 (MQ=255)
                                                                                   tttCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTc                                           <  2:3236/140‑1 (MQ=255)
                                                                                   ttacTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTc                                           <  2:938797/137‑1 (MQ=255)
                                                                                     tCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAg                                         <  1:464382/140‑1 (MQ=255)
                                                                                     tCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGC‑GCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGt                                        <  1:738294/140‑1 (MQ=255)
                                                                                      cTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGt                                        <  2:496950/140‑1 (MQ=255)
                                                                                      cTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGt                                        <  1:474751/140‑1 (MQ=255)
                                                                                      cTGACTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGt                                        <  2:498897/140‑1 (MQ=255)
                                                                                        ggCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGtaa                                      <  1:120230/140‑1 (MQ=255)
                                                                                        ggCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGtaa                                      <  2:133324/140‑1 (MQ=255)
                                                                                           tAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGtaataa                                   <  2:533265/140‑1 (MQ=255)
                                                                                             aCTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAGGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAataata                                 >  2:529887/1‑140 (MQ=255)
                                                                                             aCTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAataata                                 <  2:406338/140‑1 (MQ=255)
                                                                                              cTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAatat                                >  2:599952/1‑140 (MQ=255)
                                                                                                   tCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAg                           >  1:194130/1‑140 (MQ=255)
                                                                                                   tCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAg                           >  2:631723/1‑140 (MQ=255)
                                                                                                   tCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAg                           >  1:557201/1‑140 (MQ=255)
                                                                                                   tCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAg                           >  2:337173/1‑140 (MQ=255)
                                                                                                             cccTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTgc                 >  2:682639/1‑140 (MQ=255)
                                                                                                             cccTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTgc                 >  2:503191/1‑140 (MQ=255)
                                                                                                              ccTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTgcg                <  1:631723/140‑1 (MQ=255)
                                                                                                               ctctACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTgcgc               <  1:1169245/140‑1 (MQ=255)
                                                                                                                tctACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCa              <  1:1038566/140‑1 (MQ=255)
                                                                                                                tctACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCa              <  1:722061/140‑1 (MQ=255)
                                                                                                                tctACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCa              <  2:292713/140‑1 (MQ=255)
                                                                                                                 ctACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAc             <  1:732268/140‑1 (MQ=255)
                                                                                                                 ctACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAc             <  2:797382/140‑1 (MQ=255)
                                                                                                                 ctACCGCTTTGCGGCAAAACTCC‑‑AAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcg            <  1:757798/140‑1 (MQ=255)
                                                                                                                  tACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAGGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcg            <  1:529887/140‑1 (MQ=255)
                                                                                                                  tACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcg            <  1:682639/140‑1 (MQ=255)
                                                                                                                  tACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcg            <  2:335284/140‑1 (MQ=255)
                                                                                                                  tACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcg            <  2:649797/140‑1 (MQ=255)
                                                                                                                  tACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcg            <  2:929258/140‑1 (MQ=255)
                                                                                                                  tACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcg            <  1:303301/140‑1 (MQ=255)
                                                                                                                  tACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcg            <  1:845159/140‑1 (MQ=255)
                                                                                                                  tACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACAGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcg            <  1:91492/140‑1 (MQ=255)
                                                                                                                   aCCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTTAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcgc           <  1:32615/140‑1 (MQ=255)
                                                                                                                   aCCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcg            <  2:958916/140‑1 (MQ=255)
                                                                                                                   aCCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcgc           <  2:396876/140‑1 (MQ=255)
                                                                                                                   aCCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcgc           <  1:890086/140‑1 (MQ=255)
                                                                                                                    ccGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcgcg          <  2:676851/140‑1 (MQ=255)
                                                                                                                    ccGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcgcg          <  2:276398/140‑1 (MQ=255)
                                                                                                                    ccGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcgcg          <  2:194999/140‑1 (MQ=255)
                                                                                                                    ccGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcgcg          <  2:109564/140‑1 (MQ=255)
                                                                                                                    ccGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcgcg          <  1:328149/140‑1 (MQ=255)
                                                                                                                    ccGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcgcg          <  1:1173994/140‑1 (MQ=255)
                                                                                                                          tGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCa              >  2:551892/1‑130 (MQ=255)
                                                                                                                          tGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCa              <  1:551892/130‑1 (MQ=255)
                                                                                                                            cGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcgcg          >  1:503917/1‑132 (MQ=255)
                                                                                                                            cGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcgcg          >  1:272213/1‑132 (MQ=255)
                                                                                                                            cGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcg            <  2:846047/130‑1 (MQ=255)
                                                                                                                            cGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCAcg            >  1:846047/1‑130 (MQ=255)
                                                                                                                            cGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTatat  >  1:637784/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                 
CATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCCAAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAA‑TTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATAT  >  NC_000913/3905495‑3905759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A