Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 121 68.4 2351762 99.3% 2335299 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,101,899 A→G V70V (GTA→GTG)  kdgT → 2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,101,8990AG100.0% 180.7 / NA 53V70V (GTA→GTG) kdgT2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/25);  total (28/25)

CCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAAC  >  NC_000913/4101764‑4102021
                                                                                                                                       |                                                                                                                          
ccACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGtgctgc                                                                                                                        >  1:192118/1‑140 (MQ=255)
        tctcGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATcc                                                                                                                >  1:1013976/1‑140 (MQ=255)
        tctcGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATcc                                                                                                                >  1:255099/1‑140 (MQ=255)
        tctcGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATcc                                                                                                                <  1:515834/140‑1 (MQ=255)
        tctcGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATc                                                                                                                 >  2:552097/1‑139 (MQ=255)
        tctcGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATc                                                                                                                 <  1:552097/139‑1 (MQ=255)
        tctcGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGGGCTGCGTAAATcc                                                                                                                >  1:1002980/1‑140 (MQ=255)
           cGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGt                                                                                                             <  1:840384/140‑1 (MQ=255)
              cGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACg                                                                                                          >  1:899592/1‑140 (MQ=255)
                ggggCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGc                                                                                                         >  2:557719/1‑139 (MQ=255)
                ggggCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGc                                                                                                         <  1:557719/139‑1 (MQ=255)
                    cGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCtggtg                                                                                                    <  2:255099/140‑1 (MQ=255)
                       ggAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTa                                                                                                 <  2:1004457/140‑1 (MQ=255)
                        gAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTaa                                                                                                <  2:899592/140‑1 (MQ=255)
                                   gATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGcc                                                                                     <  1:1146957/140‑1 (MQ=255)
                                    aTCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCg                                                                                    >  2:904804/1‑140 (MQ=255)
                                        ttCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTcgc                                                                                >  2:614514/1‑140 (MQ=255)
                                        ttCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTcgc                                                                                >  1:117175/1‑140 (MQ=255)
                                                  ggATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGtt                                                                      >  2:670601/1‑140 (MQ=255)
                                                   gatgatTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTg                                                                     >  1:384545/1‑140 (MQ=255)
                                                       atTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGc                                                                 >  1:442845/1‑140 (MQ=255)
                                                       atTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGc                                                                 >  2:1132992/1‑140 (MQ=255)
                                                           ccGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAAtt                                                             <  2:760244/140‑1 (MQ=255)
                                                             ggTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGc                                                           >  1:525936/1‑140 (MQ=255)
                                                              gTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGcc                                                          <  2:442845/140‑1 (MQ=255)
                                                                aCGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTc                                                        <  1:773661/140‑1 (MQ=255)
                                                                   gTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTcgcg                                                     >  2:35419/1‑140 (MQ=255)
                                                                      cccATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGtat                                                  <  2:384545/140‑1 (MQ=255)
                                                                      cccATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGtat                                                  >  2:239169/1‑140 (MQ=255)
                                                                      cccATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGtat                                                  <  2:12898/140‑1 (MQ=255)
                                                                         aTTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTc                                                        >  2:229752/1‑131 (MQ=255)
                                                                         aTTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTc                                                        <  1:229752/131‑1 (MQ=255)
                                                                         aTTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGtattat                                               >  1:378298/1‑140 (MQ=255)
                                                                              ggCGGTGTGGTTTTTTTGGATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAAGTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCgg                                          >  2:728126/1‑140 (MQ=255)
                                                                               gCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGa                                         >  2:604940/1‑140 (MQ=255)
                                                                                cGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGaa                                        >  1:94676/1‑140 (MQ=255)
                                                                                  gtgtGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACa                                      <  1:35419/140‑1 (MQ=255)
                                                                                     tgGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGtat                                                  <  2:349864/125‑1 (MQ=255)
                                                                                     tgGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGtat                                                  >  1:349864/1‑125 (MQ=255)
                                                                                        tttttttGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGgtgt                                <  2:117175/140‑1 (MQ=255)
                                                                                             ttGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAg                           <  1:239169/140‑1 (MQ=255)
                                                                                               ggaTGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGtt                         <  1:728126/138‑1 (MQ=255)
                                                                                                          tCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGAtt                    <  1:1084278/134‑1 (MQ=255)
                                                                                                          tCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGAtt                    >  2:1084278/1‑134 (MQ=255)
                                                                                                          tCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGc              <  2:378298/140‑1 (MQ=255)
                                                                                                           cAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGcc             <  2:309758/140‑1 (MQ=255)
                                                                                                           cAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGcc             <  1:670601/140‑1 (MQ=255)
                                                                                                            aaTAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCg            >  2:1017851/1‑140 (MQ=255)
                                                                                                             aTAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCgg           <  2:192118/140‑1 (MQ=255)
                                                                                                             aTAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCgg           <  2:855569/140‑1 (MQ=255)
                                                                                                              tAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGa          >  2:1040810/1‑140 (MQ=255)
                                                                                                                      aGCGTGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAAc  >  1:725760/1‑140 (MQ=255)
                                                                                                                      aGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAAc  >  1:1123375/1‑140 (MQ=255)
                                                                                                                      aGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAAc  >  2:109767/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                          
CCACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAAC  >  NC_000913/4101764‑4102021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A