Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	121	68.4	2351762	99.3%	2335299	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,611,779	G→A	S128S (TCG→TCA)	osmY →	periplasmic chaperone OsmY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,611,779	0	G	A	100.0%	191.2 / NA	55	S128S (TCG→TCA)	osmY	periplasmic chaperone OsmY
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (32/23); total (32/23)

GATCAAAAAGTCGTGACCCTGAGCGGTTTCGTTGAAAGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCGGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGACGGCGTGGTTCAGCTCTCCGGTACCGTCGATT  >  NC_000913/4611642‑4611915

gATCAAAAAGTCGTGACCCTGAGCGGTTTCGTTGAAAGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGt                                                                                                                                        >  2:670515/1‑140 (MQ=255)

gATCAAAAAGTCGTGACCCTGAGCGGTTTCGTTGAAAGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGt                                                                                                                                        >  2:527987/1‑140 (MQ=255)

gATCAAAAAGTCGTGACCCTGAGCGGTTTCGTTGAAAGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGt                                                                                                                                        >  1:641336/1‑140 (MQ=255)

 aTCAAAAAGTCGTGACCCTGAGCGGTTTCGTTGAAAGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTg                                                                                                                                       >  2:711476/1‑140 (MQ=255)

  tCAAAAAGTCGTGACCCTGAGCGGTTTCGTTGAAAGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGa                                                                                                                                      >  2:141467/1‑140 (MQ=255)

           cGTGACCCTGAGCGGTTTCGTTGAAAGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTAcg                                                                                                                             <  2:1151377/140‑1 (MQ=255)

            gTGACCCTGAGCGGTTTCGTTGAAAGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTAcgc                                                                                                                            >  1:274525/1‑140 (MQ=255)

            gTGACCCTGAGCGGTTTCGTTGAAAGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTAcgc                                                                                                                            <  1:670515/140‑1 (MQ=255)

             tGACCCTGAGCGGTTTCGTTGAAAGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTAcgcg                                                                                                                           >  2:62282/1‑140 (MQ=255)

              gACCCTGAGCGGTTTCGTTGAAAGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCgg                                                                                                                          <  2:112131/140‑1 (MQ=255)

                  cTGAGCGGTTTCGTTGAAAGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGa                                                                                                                      <  1:379832/140‑1 (MQ=255)

                        ggTTTCGTTGAAAGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGc                                                                                                                <  1:527987/140‑1 (MQ=255)

                         gTTTCGTTGAAAGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGcc                                                                                                               <  1:1103496/140‑1 (MQ=255)

                          tttCGTTGAAAGTCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGcca                                                                                                              >  1:508218/1‑140 (MQ=255)

                             cGTTGAAAGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGccacca                                                                                                           <  2:274525/140‑1 (MQ=255)

                                   aaGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTg                                                                                                     <  2:1094727/140‑1 (MQ=255)

                                    aGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGa                                                                                                    >  1:466212/1‑140 (MQ=255)

                                            ccaggccGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAg                                                                                            >  2:723410/1‑140 (MQ=255)

                                                gccGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCaa                                                                                        >  1:474427/1‑140 (MQ=255)

                                                     agagGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAActgc                                                                                   >  2:389445/1‑140 (MQ=255)

                                                           aGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCgg                                                                             >  1:13596/1‑140 (MQ=255)

                                                            gTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGa                                                                            <  2:693275/140‑1 (MQ=255)

                                                                 aGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGata                                                                       <  2:466212/140‑1 (MQ=255)

                                                                   tGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGGGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATc                                                                     >  1:242578/1‑140 (MQ=255)

                                                                    ggCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCg                                                                    <  2:1150106/140‑1 (MQ=255)

                                                                    ggCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCg                                                                    >  1:1150106/1‑140 (MQ=255)

                                                                     gCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGt                                                                   >  1:308437/1‑140 (MQ=255)

                                                                      cGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTc                                                                  <  1:389445/140‑1 (MQ=255)

                                                                       gAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAActgctg                                                                                 <  2:107113/124‑1 (MQ=255)

                                                                       gAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAActgctg                                                                                 >  1:107113/1‑124 (MQ=255)

                                                                                      ggTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGa                                                  <  2:308437/140‑1 (MQ=255)

                                                                                       gTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTccc                                                                <  1:660673/125‑1 (MQ=255)

                                                                                       gTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTccc                                                                >  2:660673/1‑125 (MQ=255)

                                                                                       gTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGaa                                                 >  2:517143/1‑140 (MQ=255)

                                                                                       gTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGaa                                                 >  1:1115435/1‑140 (MQ=255)

                                                                                       gTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGaa                                                 >  1:23056/1‑140 (MQ=255)

                                                                                         gACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAg                                               >  2:690843/1‑140 (MQ=255)

                                                                                          aCCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGt                                              >  2:260131/1‑140 (MQ=255)

                                                                                               tGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGATGATATCGTCCCTTCCCGTCATGTGAAAGTTGaaa                                         >  1:492856/1‑140 (MQ=255)

                                                                                                 tCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAacc                                       >  2:1129272/1‑140 (MQ=255)

                                                                                                      gACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGa                                  <  2:44831/140‑1 (MQ=255)

                                                                                                         aaaCTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGACgg                               >  2:219736/1‑140 (MQ=255)

                                                                                                           aCTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGACGGCg                             <  2:13596/140‑1 (MQ=255)

                                                                                                           aCTGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGACGGCg                             <  1:690843/140‑1 (MQ=255)

                                                                                                             tGCACGTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGACGGCGTg                           >  1:212967/1‑140 (MQ=255)

                                                                                                                  gTTCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCA‑CAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGACGGCGTGGTTCAg                     <  2:1131511/140‑1 (MQ=255)

                                                                                                                   ttCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGACGGCGTGGTTCAg                     <  2:23056/140‑1 (MQ=255)

                                                                                                                   ttCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGACGGCGTGGTTCAg                     <  2:1115435/140‑1 (MQ=255)

                                                                                                                   ttCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGACGGCGTGGTTCAg                     <  1:1129272/140‑1 (MQ=255)

                                                                                                                   ttCGCGACGCTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGACGGCGTGGTTCAg                     <  1:62282/140‑1 (MQ=255)

                                                                                                                            cTAAAGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGACGGCGTGGTTCAGCTCTCCGGt            >  1:790564/1‑140 (MQ=255)

                                                                                                                              aaaGAAGGCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGACGGCGTGGTTCAGCTCTCCGGTAc          >  2:263562/1‑140 (MQ=255)

                                                                                                                                    ggCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGACGGCGTGGTTCAGCTCTCCGGTACCGTCGa    >  1:942909/1‑140 (MQ=255)

                                                                                                                                     gCTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGACGGCGTGGTTCAGCTCTCCGGTACCGTCGAt   >  1:98343/1‑140 (MQ=255)

                                                                                                                                      cTCAGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGACGGCGTGGTTCAGCTCTCCGGTACCGTCGAtt  >  2:1098063/1‑140 (MQ=255)

GATCAAAAAGTCGTGACCCTGAGCGGTTTCGTTGAAAGCCAGGCCCAGGCCGAAGAGGCAGTGAAAGTGGCGAAAGGCGTTGAAGGGGTGACCTCTGTCAGCGACAAACTGCACGTTCGCGACGCTAAAGAAGGCTCGGTGAAGGGCTACGCGGGTGACACCGCCACCACCAGTGAAATCAAAGCCAAACTGCTGGCGGACGATATCGTCCCTTCCCGTCATGTGAAAGTTGAAACCACCGACGGCGTGGTTCAGCTCTCCGGTACCGTCGATT  >  NC_000913/4611642‑4611915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A