Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	120	93.5	3664254	88.3%	3235536	136.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,769,302	G→A	V77M (GTG→ATG)	ydiK →	putative transporter YdiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,769,302	0	G	A	100.0%	189.0 / NA	53	V77M (GTG→ATG)	ydiK	putative transporter YdiK
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/30); total (23/30)

CAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCG  >  NC_000913/1769167‑1769434

cAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGatgatg                                                                                                                                    >  1:117991/1‑138 (MQ=255)

cAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGatgatg                                                                                                                                    >  1:1597236/1‑138 (MQ=255)

   cccTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCCGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGttt                                                                                                                                 <  2:1597236/138‑1 (MQ=255)

    ccTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTa                                                                                                                                <  1:1250793/138‑1 (MQ=255)

      tttATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGNTGATGACGCTGTTATTAGTGATGATGTTTatc                                                                                                                              <  1:408639/138‑1 (MQ=255)

      tttATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTatc                                                                                                                              <  2:16055/138‑1 (MQ=255)

      tttATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTatc                                                                                                                              <  1:1209728/138‑1 (MQ=255)

        tATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTa                                                                                                                                >  2:230945/1‑134 (MQ=255)

        tATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTa                                                                                                                                <  1:230945/134‑1 (MQ=255)

          ttCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATcc                                                                                                                          >  1:1293681/1‑138 (MQ=255)

          ttCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATcc                                                                                                                          >  1:252430/1‑138 (MQ=255)

                gCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTa                                                                                                                                <  2:1259244/126‑1 (MQ=255)

                gCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTa                                                                                                                                >  1:1259244/1‑126 (MQ=255)

                 cTTTGCATGGGCCGGTACGGTGGTTATCGCCAGCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTAttgc                                                                                                                   >  2:1623489/1‑138 (MQ=255)

                 cTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTAttgc                                                                                                                   >  2:858040/1‑138 (MQ=255)

                 cTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTAttgc                                                                                                                   <  1:799068/138‑1 (MQ=255)

                   ttGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTAttgctt                                                                                                                 <  2:761442/138‑1 (MQ=255)

                   ttGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTAttgctt                                                                                                                 <  1:1623489/138‑1 (MQ=255)

                      cATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTAttgctttgc                                                                                                              <  2:1293681/138‑1 (MQ=255)

                      cATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTAttgctttgc                                                                                                              <  2:1037892/138‑1 (MQ=255)

                      cATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTAttgctttgc                                                                                                              <  2:252430/138‑1 (MQ=255)

                         gggCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTgg                                                                                                           <  1:926731/138‑1 (MQ=255)

                         gggCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTgg                                                                                                           <  2:117991/138‑1 (MQ=255)

                           gCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGtt                                                                                                         <  1:922364/138‑1 (MQ=255)

                                tACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAg                                                                                                    <  1:858040/138‑1 (MQ=255)

                                  cGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTa                                                                                                  >  1:422144/1‑138 (MQ=255)

                                  cGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTa                                                                                                  >  2:1168278/1‑138 (MQ=255)

                                       gtTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTAtcgtc                                                                                             >  1:384945/1‑138 (MQ=255)

                                            cGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACgg                                                                                        >  1:710947/1‑138 (MQ=255)

                                               cACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAg                                                                                     <  2:710947/138‑1 (MQ=255)

                                                 ccTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCg                                                                                   <  2:422144/138‑1 (MQ=255)

                                                                      tGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCAttt                                                              >  1:1489549/1‑138 (MQ=255)

                                                                       gCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTc                                                             <  2:1359582/138‑1 (MQ=255)

                                                                          aaaGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAg                                                          <  2:246778/138‑1 (MQ=255)

                                                                           aaGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTa                                                                       <  2:1125335/124‑1 (MQ=255)

                                                                           aaGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTa                                                                       >  1:1125335/1‑124 (MQ=255)

                                                                               tCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTg                                                     >  1:344189/1‑138 (MQ=255)

                                                                                  tGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACa                                                  >  1:1071656/1‑138 (MQ=255)

                                                                                         ccgccgCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGtt                                           >  2:1519215/1‑138 (MQ=255)

                                                                                          cgccgcTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTa                                          >  1:54497/1‑138 (MQ=255)

                                                                                          cgccgcTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTa                                          >  1:1239926/1‑138 (MQ=255)

                                                                                               ctctctCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGa                                     >  1:1076928/1‑138 (MQ=255)

                                                                                               ctctctCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGa                                     >  1:992390/1‑138 (MQ=255)

                                                                                               ctctctCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGa                                     >  1:22165/1‑138 (MQ=255)

                                                                                                    tcGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTgg                                <  2:1076928/138‑1 (MQ=255)

                                                                                                    tcGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTgg                                <  2:267103/138‑1 (MQ=255)

                                                                                                    tcGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTgg                                <  2:384945/138‑1 (MQ=255)

                                                                                                    tcGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTgg                                <  1:1168278/138‑1 (MQ=255)

                                                                                                     cGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGa                                               <  2:515915/122‑1 (MQ=255)

                                                                                                     cGCCGTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGa                                               >  1:515915/1‑122 (MQ=255)

                                                                                                         gTTCTGGTGATGACGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTgg                           <  2:568440/138‑1 (MQ=255)

                                                                                                                 gatgaCGCTGTTATTAGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATAc                   <  2:1239926/138‑1 (MQ=255)

                                                                                                                           ttattaGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTgg                           >  1:88695/1‑120 (MQ=255)

                                                                                                                           ttattaGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTgg                           <  2:88695/120‑1 (MQ=255)

                                                                                                                           ttattaGTGATGATGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTg         >  1:804958/1‑138 (MQ=255)

                                                                                                                                  tgatgaTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGgcg  <  2:344189/138‑1 (MQ=255)

CAACCCTTTATTCTCGGCTTTGCATGGGCCGGTACGGTGGTTATCGCCACCTGGCCGGTATTGTTACGTTTGCAAAAGATCATGTTTGGCCGCCGCTCTCTCGCCGTTCTGGTGATGACGCTGTTATTAGTGATGGTGTTTATCATCCCTATTGCTTTGCTGGTTAACAGTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCG  >  NC_000913/1769167‑1769434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A