Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I1 R1 120 93.5 3664254 88.3% 3235536 136.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,562,631 T→C R36R (CGT→CGC)  yffP → CPZ‑55 prophage; uncharacterized protein YffP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,562,6310TC98.9% 324.0 / ‑5.3 88R36R (CGT→CGC) yffPCPZ‑55 prophage; uncharacterized protein YffP
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (46/41);  total (47/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTCACCATTTGGAGATGACAAACAATGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGTTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTGACGACTGCCTGGTAATGACACG  >  NC_000913/2562498‑2562762
                                                                                                                                     |                                                                                                                                   
cATTCACCATTTGGAGATGACAAACAATGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTa                                                                                                                                 >  1:1651049/1‑138 (MQ=255)
 aTTCACCATTTGGAGATGACAAACAATGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTaa                                                                                                                                <  1:1293251/138‑1 (MQ=255)
     aCCATTTGGAGATGACAAACAATGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTg                                                                                                                            >  1:598984/1‑138 (MQ=255)
     aCCATTTGGAGATGACAAACAATGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTg                                                                                                                            >  1:90549/1‑138 (MQ=255)
           tGGAGATGACAAACAATGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGAc                                                                                                                      >  2:115582/1‑138 (MQ=255)
            ggAGATGACAAACAATGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGAcg                                                                                                                     >  2:265435/1‑138 (MQ=255)
            ggAGATGACAAACAATGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGAcg                                                                                                                     >  2:1477697/1‑138 (MQ=255)
              agaTGACAAACAATGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGAcgcg                                                                                                                   >  2:875182/1‑138 (MQ=255)
               gaTGACAAACAATGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGTTTTAATCTGATAGACgcgc                                                                                                                  >  1:1293439/1‑138 (MQ=255)
                 tGACAAACAATGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGAGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGctt                                                                                                                <  2:1746630/138‑1 (MQ=255)
                 tGACAAACAATGAGCCTGATCCGCACTGAAACAAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGctt                                                                                                                <  2:1106093/138‑1 (MQ=255)
                  gACAAACAATGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGcttc                                                                                                               >  2:1771658/1‑138 (MQ=255)
                   acaaacaaTGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGcttct                                                                                                              >  1:846559/1‑138 (MQ=255)
                     aaacaaTGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGTTTTAATCTGATAGACGCGCTTCTTg                                                                                                            <  2:1293439/138‑1 (MQ=255)
                     aaacaaTGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTg                                                                                                            <  2:1624584/138‑1 (MQ=255)
                     aaacaaTGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTg                                                                                                            <  2:90549/138‑1 (MQ=255)
                     aaacaaTGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTg                                                                                                            <  2:1721272/138‑1 (MQ=255)
                     aaacaaTGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTg                                                                                                            <  1:265435/138‑1 (MQ=255)
                     aaacaaTGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTg                                                                                                            >  2:665365/1‑138 (MQ=255)
                         aaTGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGtt                                                                                                        <  2:406958/138‑1 (MQ=255)
                         aaTGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGtt                                                                                                        <  2:1651049/138‑1 (MQ=255)
                         aaTGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGtt                                                                                                        <  2:1221634/138‑1 (MQ=255)
                          aTGAGCCTGATCCGCACTGAAGCCCGCGACACAAAACGCGTCGCTGACCCACTGCACTAACTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGGCATCTGGCGTTTTAATCTGATCGACGTGCTTCTTGAGttt                                                                                                       <  2:525450/138‑1 (MQ=255)
                          aTGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGttt                                                                                                       >  1:1094990/1‑138 (MQ=255)
                             aGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTc                                                                                                    >  2:1561231/1‑138 (MQ=255)
                               ccTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTa                                                                                                  <  1:1218272/138‑1 (MQ=255)
                                 tGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTg                                                                                                            <  1:1005573/126‑1 (MQ=255)
                                 tGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTg                                                                                                            >  2:1005573/1‑126 (MQ=255)
                                   aTCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTAcaca                                                                                              <  2:1598858/138‑1 (MQ=255)
                                   aTCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTAcaca                                                                                              <  1:115582/138‑1 (MQ=255)
                                   aTCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTAcaca                                                                                              >  2:1740721/1‑138 (MQ=255)
                                   aTCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTAcaca                                                                                              <  1:1561675/138‑1 (MQ=255)
                                     ccGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTAcaca                                                                                              <  1:1809125/136‑1 (MQ=255)
                                     ccGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTAcaca                                                                                              >  2:1809125/1‑136 (MQ=255)
                                     ccGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGa                                                                                            >  2:605904/1‑138 (MQ=255)
                                     ccGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGa                                                                                            >  1:1671485/1‑138 (MQ=255)
                                         aCTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGAcaca                                                                                        >  2:452947/1‑138 (MQ=255)
                                         aCTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGAcaca                                                                                        >  2:1225364/1‑138 (MQ=255)
                                          cTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACAt                                                                                       >  1:85011/1‑138 (MQ=255)
                                            gAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATcc                                                                                     >  1:1170392/1‑138 (MQ=255)
                                             aaaCCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCa                                                                                    <  2:1477130/138‑1 (MQ=255)
                                               aCCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATcc                                                                                     >  1:161589/1‑135 (MQ=255)
                                               aCCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATcc                                                                                     <  2:161589/135‑1 (MQ=255)
                                               aCCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGc                                                                                  >  2:1367579/1‑138 (MQ=255)
                                               aCCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAAATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGc                                                                                  >  2:909475/1‑138 (MQ=255)
                                                  agagACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCg                                                                               <  1:665365/138‑1 (MQ=255)
                                                    agaCACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATc                                                                                      <  2:152500/129‑1 (MQ=255)
                                                    agaCACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATc                                                                                      >  1:152500/1‑129 (MQ=255)
                                                           aaaCGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCa                                                                                    >  1:1525309/1‑124 (MQ=255)
                                                           aaaCGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCa                                                                                    <  2:1525309/124‑1 (MQ=255)
                                                             aCGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCgg                                                                    <  2:724411/138‑1 (MQ=255)
                                                             aCGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCgg                                                                    <  1:605904/138‑1 (MQ=255)
                                                              cgcgCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGc                                                                   <  2:846559/138‑1 (MQ=255)
                                                                  cAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGa                                                               <  1:1395328/138‑1 (MQ=255)
                                                                  cAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGa                                                               <  2:85011/138‑1 (MQ=255)
                                                                    gCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGAcc                                                             >  1:86204/1‑138 (MQ=255)
                                                                          ccACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGAt                                                       >  1:335635/1‑138 (MQ=255)
                                                                              tGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTaca                                                   <  1:1477697/138‑1 (MQ=255)
                                                                                   gACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAg                                              <  2:1642624/138‑1 (MQ=255)
                                                                                    aCCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGt                                             >  2:1054488/1‑138 (MQ=255)
                                                                                    aCCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGt                                             <  1:1367579/138‑1 (MQ=255)
                                                                                    aCCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGt                                             <  1:399099/138‑1 (MQ=255)
                                                                                     ccTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTg                                            >  2:1024936/1‑138 (MQ=255)
                                                                                          gTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTaca                                                   <  1:101613/126‑1 (MQ=255)
                                                                                          gTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTaca                                                   >  2:809551/1‑126 (MQ=255)
                                                                                          gTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTaca                                                   <  1:809551/126‑1 (MQ=255)
                                                                                          gTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTaca                                                   >  2:101613/1‑126 (MQ=255)
                                                                                          gTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTggg                                       <  1:875182/138‑1 (MQ=255)
                                                                                          gTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTggg                                       >  1:748690/1‑138 (MQ=255)
                                                                                          gTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTggg                                       >  2:688584/1‑138 (MQ=255)
                                                                                                  ccGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGgt                               >  1:196606/1‑138 (MQ=255)
                                                                                                   cGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGgtg                              >  1:900701/1‑138 (MQ=255)
                                                                                                     ttttCTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGtt                            >  2:1170228/1‑138 (MQ=255)
                                                                                                         cTGAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTg                        >  1:1581694/1‑138 (MQ=255)
                                                                                                           gAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTgac                      <  2:1170392/138‑1 (MQ=255)
                                                                                                           gAGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTgac                      <  1:1561231/138‑1 (MQ=255)
                                                                                                            aGTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTgacg                     <  1:1225364/138‑1 (MQ=255)
                                                                                                             gTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTgacga                    <  2:1671485/138‑1 (MQ=255)
                                                                                                             gTGGGGCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTgacga                    >  2:1605988/1‑138 (MQ=255)
                                                                                                               ggggCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTGACGACt                  >  2:1541788/1‑138 (MQ=255)
                                                                                                                gggCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTGACGACTg                 <  2:748690/138‑1 (MQ=255)
                                                                                                                 ggCGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTGACGACTGc                >  1:1501840/1‑138 (MQ=255)
                                                                                                                   cGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTgac                      <  1:1536908/130‑1 (MQ=255)
                                                                                                                   cGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTgac                      >  2:1536908/1‑130 (MQ=255)
                                                                                                                   cGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTGACGACTg                 <  1:1649391/135‑1 (MQ=255)
                                                                                                                   cGAAGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTGACGACTg                 >  2:1649391/1‑135 (MQ=255)
                                                                                                                     aaGATGAAATCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTGACGACTGCCTgg            >  2:202597/1‑138 (MQ=255)
                                                                                                                             aTCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTGACGACTGCCTGGTAATGaca    >  1:1604087/1‑138 (MQ=255)
                                                                                                                             aTCAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTGACGACTGCCTGGTAATGaca    >  2:1051109/1‑138 (MQ=255)
                                                                                                                               cAGGCGCTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTGACGACTGCCTGGTAATGACAcg  <  1:202597/138‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
CATTCACCATTTGGAGATGACAAACAATGAGCCTGATCCGCACTGAAACCAGAGACACAAAACGCGCAGCTGACCCACTGCACGACCTGCGTAGCAAGCCGTTTTCTGAGTGGGGCGAAGATGAAATCAGGCGTTTTAATCTGATAGACGCGCTTCTTGAGTTTGTCTACACAGACACATCCAGCCCGTTCGGTATCGGCATGACCTTCGATTACACGGAGTGCTGGGAAATTGGTGTTCGTGACGACTGCCTGGTAATGACACG  >  NC_000913/2562498‑2562762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A