Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	120	93.5	3664254	88.3%	3235536	136.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,103,609	T→C	C199C (TGT→TGC)	mutY →	adenine DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,103,609	0	T	C	100.0%	211.0 / NA	57	C199C (TGT→TGC)	mutY	adenine DNA glycosylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/33); total (24/33)

CCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGTTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCACGAAGA  >  NC_000913/3103475‑3103737

ccTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCg                                                                                                                               <  1:1472701/138‑1 (MQ=255)

ccTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCg                                                                                                                               <  2:230509/138‑1 (MQ=255)

     gAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTg                                                                                                                          <  2:875323/138‑1 (MQ=255)

     gAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTg                                                                                                                          <  1:927435/138‑1 (MQ=255)

     gAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTg                                                                                                                          <  1:1419793/138‑1 (MQ=255)

     gAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTg                                                                                                                          <  2:1673453/138‑1 (MQ=255)

     gAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTg                                                                                                                          <  2:247882/138‑1 (MQ=255)

          aaGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGc                                                                                                                     <  1:1264143/138‑1 (MQ=255)

                 cGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACaaaa                                                                                                              >  2:747784/1‑138 (MQ=255)

                          aTTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCCCTGTCCGCTACAAAACGGATGTAt                                                                                                     <  1:1825099/138‑1 (MQ=255)

                               ggAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTgccg                                                                                                <  2:1811655/138‑1 (MQ=255)

                                gAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTgccgc                                                                                               <  1:747784/138‑1 (MQ=255)

                                gAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTgccgc                                                                                               <  1:1527610/138‑1 (MQ=255)

                                 aGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTgccgcc                                                                                              >  2:1568552/1‑138 (MQ=255)

                                 aGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCCCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTgccgcc                                                                                              >  2:882914/1‑138 (MQ=255)

                                     tGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCca                                                                                          >  2:148381/1‑138 (MQ=255)

                                     tGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCca                                                                                          >  2:275575/1‑138 (MQ=255)

                                       agcgagcAGGTTACGACCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGCTGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAACTGTTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCcaac                                                                                        >  2:1171421/1‑138 (MQ=255)

                                       agcgagcAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCcaac                                                                                        >  2:158930/1‑138 (MQ=255)

                                             cAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTgccgc                                                                                               >  1:1676602/1‑125 (MQ=255)

                                             cAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTgccgc                                                                                               <  2:1676602/125‑1 (MQ=255)

                                             cAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGc                                                                                  <  1:158930/138‑1 (MQ=255)

                                                        cgcgGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTa                                                                       >  1:468716/1‑138 (MQ=255)

                                                        cgcgGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTa                                                                       >  2:1147154/1‑138 (MQ=255)

                                                          cgGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATc                                                                     >  1:904885/1‑138 (MQ=255)

                                                            gTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCg                                                                   >  2:422083/1‑138 (MQ=255)

                                                            gTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCg                                                                   >  1:841729/1‑138 (MQ=255)

                                                                 cGTGGAACGGTCTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCaa                                                              <  1:1076202/138‑1 (MQ=255)

                                                                         ggTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGa                                                      <  1:438301/138‑1 (MQ=255)

                                                                           tttAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGaaa                                                    <  2:841729/138‑1 (MQ=255)

                                                                               aTCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGa                                                <  1:275575/138‑1 (MQ=255)

                                                                                     cGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGc                                          >  2:1592040/1‑138 (MQ=255)

                                                                                     cGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGc                                          <  2:1185214/138‑1 (MQ=255)

                                                                                      gatgatGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGcc                                         >  2:1498823/1‑138 (MQ=255)

                                                                                       atgatgGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCg                                        <  2:904885/138‑1 (MQ=255)

                                                                                         gatgGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGa                                      <  1:1240626/138‑1 (MQ=255)

                                                                                                ttGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAgcg                                   >  2:1297019/1‑134 (MQ=255)

                                                                                                ttGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAgcg                                   <  1:1297019/134‑1 (MQ=255)

                                                                                                ttGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCAcc                               >  2:481509/1‑138 (MQ=255)

                                                                                                    gTGCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCt                           >  1:1401200/1‑138 (MQ=255)

                                                                                                      gCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTAc                         <  1:1147154/138‑1 (MQ=255)

                                                                                                      gCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTAc                         <  1:1592040/138‑1 (MQ=255)

                                                                                                      gCGATGATTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTAc                         >  2:1056628/1‑138 (MQ=255)

                                                                                                        gatgatTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTACtt                       <  1:481509/138‑1 (MQ=255)

                                                                                                        gatgatTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTACtt                       <  1:828039/138‑1 (MQ=255)

                                                                                                         atgatTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCg                              <  1:578509/130‑1 (MQ=255)

                                                                                                         atgatTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCg                              >  2:578509/1‑130 (MQ=255)

                                                                                                          tgatTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTACtttt                     <  1:1498823/138‑1 (MQ=255)

                                                                                                          tgatTTGTACGCGCTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTACtttt                     >  2:795488/1‑138 (MQ=255)

                                                                                                                       cTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCa        <  1:1512378/138‑1 (MQ=255)

                                                                                                                       cTCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCa        <  1:882914/138‑1 (MQ=255)

                                                                                                                        tCGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCAc       >  2:398937/1‑138 (MQ=255)

                                                                                                                         cGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCa        <  1:151817/136‑1 (MQ=255)

                                                                                                                         cGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCa        >  2:151817/1‑136 (MQ=255)

                                                                                                                         cGAAACCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCACg      >  2:1271579/1‑138 (MQ=255)

                                                                                                                           aaaCCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCACGaa    <  1:422083/138‑1 (MQ=255)

                                                                                                                           aaaCCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCACGaa    >  2:1487794/1‑138 (MQ=255)

                                                                                                                             aCCGAAATGCTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCACGAAGa  <  2:546505/138‑1 (MQ=255)

CCTGGGAAAAAAGAGGTCGAGAATAAATTATGGAGTTTGAGCGAGCAGGTGACGCCCGCGGTTGGCGTGGAACGGTTTAATCAGGCGATGATGGATTTGGGTGCGATGATTTGTACGCGCTCGAAACCGAAATGTTCGCTCTGTCCGCTACAAAACGGATGTATTGCCGCCGCCAACAATAGCTGGGCGCTTTATCCGGGCAAAAAACCGAAACAGACGCTGCCGGAGCGCACCGGCTACTTTTTGCTATTACAGCACGAAGA  >  NC_000913/3103475‑3103737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A