Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	120	93.5	3664254	88.3%	3235536	136.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,385,668	G→A	G44D (GGT→GAT)	yhcN →	DUF1471 domain‑containing stress‑induced protein YhcN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,385,668	0	G	A	100.0%	212.8 / NA	60	G44D (GGT→GAT)	yhcN	DUF1471 domain‑containing stress‑induced protein YhcN
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (29/31); total (29/31)

ATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAA  >  NC_000913/3385538‑3385801

aTGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCg                                                                                                                                <  2:207386/138‑1 (MQ=255)

  gAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTc                                                                                                                              <  1:353876/138‑1 (MQ=255)

     aaTCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTc                                                                                                                           <  1:1784668/138‑1 (MQ=255)

            aCCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTTCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATg                                                                                                                    <  2:1504196/138‑1 (MQ=255)

            aCCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATg                                                                                                                    <  2:1214495/138‑1 (MQ=255)

              cACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGa                                                                                                                  <  1:478282/138‑1 (MQ=255)

                               gCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGa                                                                                                 >  2:690062/1‑138 (MQ=255)

                                     tttCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACaaaa                                                                                           >  2:521660/1‑138 (MQ=255)

                                     tttCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACaaaa                                                                                           >  1:20575/1‑138 (MQ=255)

                                     tttCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACaaaa                                                                                           >  2:1453787/1‑138 (MQ=255)

                                        cTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACaaaaa                                                                                          >  2:914369/1‑136 (MQ=255)

                                        cTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACaaaaa                                                                                          <  1:914369/136‑1 (MQ=255)

                                                  tttCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGaa                                                                              >  2:798716/1‑138 (MQ=255)

                                                     cGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACaaaaa                                                                                          <  1:1639562/123‑1 (MQ=255)

                                                     cGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACaaaaa                                                                                          >  2:1639562/1‑123 (MQ=255)

                                                       gTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGgcg                                                                         <  2:20575/138‑1 (MQ=255)

                                                        tGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGgcgc                                                                        <  2:1026944/138‑1 (MQ=255)

                                                         gCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCa                                                                       <  1:798716/138‑1 (MQ=255)

                                                          cATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATAGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGgcgc                                                                        >  2:330771/1‑136 (MQ=255)

                                                          cATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATAGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGgcgc                                                                        <  1:330771/136‑1 (MQ=255)

                                                            ttagCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACg                                                                    >  2:345060/4‑138 (MQ=255)

                                                            ttCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACg                                                                    >  2:1482792/1‑138 (MQ=255)

                                                              cGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGc                                                                  >  2:1137508/1‑138 (MQ=255)

                                                               gCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGcc                                                                 <  1:1093762/138‑1 (MQ=255)

                                                               gCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGcc                                                                 <  1:1145111/138‑1 (MQ=255)

                                                                cTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCt                                                                <  1:595153/138‑1 (MQ=255)

                                                                cTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCt                                                                <  1:1627206/138‑1 (MQ=255)

                                                                               aTGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAg                                                 >  1:841278/1‑138 (MQ=255)

                                                                                   tgcACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCg                                             >  1:209158/1‑138 (MQ=255)

                                                                                    gcacaagcacaaAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGt                                            >  1:1269219/1‑138 (MQ=255)

                                                                                      acaagcacaaAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAGTGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAg                                          >  2:1805919/1‑138 (MQ=255)

                                                                                      acaagcacaaAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAg                                          >  1:1071924/1‑138 (MQ=255)

                                                                                      acaagcacaaAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAg                                          >  2:502343/1‑138 (MQ=255)

                                                                                      acaagcacaaAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAg                                          >  1:189229/1‑138 (MQ=255)

                                                                                      acaagcacaaAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAg                                          >  1:771643/1‑138 (MQ=255)

                                                                                       caagcacaaAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGc                                         >  2:434838/1‑138 (MQ=255)

                                                                                       caagcacaaAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGc                                         >  2:1475396/1‑138 (MQ=255)

                                                                                       caagcacaaAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGc                                         >  1:1545858/1‑138 (MQ=255)

                                                                                            acaaAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGa                                    <  2:1675882/138‑1 (MQ=255)

                                                                                            acaaAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGa                                    >  1:83872/1‑138 (MQ=255)

                                                                                            acaaAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGa                                    >  2:1065562/1‑138 (MQ=255)

                                                                                               aaaTCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGTGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGacac                                 >  1:1222243/1‑138 (MQ=255)

                                                                                               aaaTCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGacac                                 >  1:441012/1‑138 (MQ=255)

                                                                                               aaaTCGTGAAGCAATCGGGACCGTATCAGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGacac                                 >  2:1714858/1‑138 (MQ=255)

                                                                                                 aTCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCt                               <  1:859595/138‑1 (MQ=255)

                                                                                                 aTCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCt                               <  2:630846/138‑1 (MQ=255)

                                                                                                 aTCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCt                               <  1:434838/138‑1 (MQ=255)

                                                                                                 aTCGTGAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCt                               <  1:1434288/138‑1 (MQ=255)

                                                                                                      gAAGCAATCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCAc                          <  2:83872/138‑1 (MQ=255)

                                                                                                           aaTCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTAc                     <  1:345060/138‑1 (MQ=255)

                                                                                                             tCGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACgg                   <  2:1545858/138‑1 (MQ=255)

                                                                                                              cGGGACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGc                  <  1:1541573/138‑1 (MQ=255)

                                                                                                                ggACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTg                <  2:1756685/138‑1 (MQ=255)

                                                                                                                ggACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTg                <  1:1137508/138‑1 (MQ=255)

                                                                                                                ggACCGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTg                <  2:1269219/138‑1 (MQ=255)

                                                                                                                ggACCGTATCAGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTg                <  1:1714858/138‑1 (MQ=255)

                                                                                                                   ccGTATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAAc             <  1:1494424/138‑1 (MQ=255)

                                                                                                                      tATCCGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGt          >  2:217802/1‑138 (MQ=255)

                                                                                                                          cGTAAGTGATGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACaa      >  1:1648201/1‑138 (MQ=255)

                                                                                                                              aGTGATGTGGCGTCTTCGCCAGTGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATaa  <  1:1805919/138‑1 (MQ=255)

ATGAAAATCAAAACCACTGTTGCTGCATTAAGCGTACTTTCTGTTCTCTCTTTCGGTGCATTCGCTGCCGACTCCATTGATGCTGCACAAGCACAAAATCGTGAAGCAATCGGGACCGTATCCGTAAGTGGTGTGGCGTCTTCGCCAATGGATATGCGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAA  >  NC_000913/3385538‑3385801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A