Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 191 83.5 2830284 99.3% 2810472 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,524,964 T→C A339A (GCA→GCG)  ansP ← L‑asparagine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,524,9640TC100.0% 179.3 / NA 50A339A (GCA→GCG) ansPL‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/22);  total (28/22)

CACAATCTCAAATACGCGCGACGGCACCAGATAGTTGAGGAATACGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTT  >  NC_000913/1524838‑1525091
                                                                                                                              |                                                                                                                               
cacaATCTCAAATACGCGCGACGGCACCAGATAGTTGAGGAATACGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCa                                                                                                                    <  1:1297446/140‑1 (MQ=255)
             aCGCGCGACGGCACCAGATAGTTGAGGAATACGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAgcg                                                                                                       <  1:1120236/140‑1 (MQ=255)
             aCGCGCGACGGCACCAGATAGTTGAGGAATACGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAgcg                                                                                                       <  1:1233255/140‑1 (MQ=255)
               gcgcgACGGCACCAGATAGTTGAGGAATACGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCAt                                                                                                                   <  2:524951/126‑1 (MQ=255)
               gcgcgACGGCACCAGATAGTTGAGGAATACGCCGACGACATACACAACTAGTGTCACCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCAt                                                                                                                   >  1:524951/1‑126 (MQ=255)
                     cGGCACCAGATAGTTGAGGAATACGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATAc                                                                                               >  2:1318894/1‑140 (MQ=255)
                        cACCAGATAGTTGAGGAATACGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTc                                                                                            <  1:1239065/140‑1 (MQ=255)
                        cACCAGATAGTTGAGGAATACGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTc                                                                                            <  1:1264229/140‑1 (MQ=255)
                        cACCAGATAGTTGAGGAATACGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTc                                                                                            <  1:1318894/140‑1 (MQ=255)
                           cAGATAGTTGAGGAATACGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAg                                                                                                         >  2:182315/1‑124 (MQ=255)
                           cAGATAGTTGAGGAATACGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAg                                                                                                         <  1:182315/124‑1 (MQ=255)
                             gATAGTTGAGGAATACGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTg                                                                                       <  2:1199533/140‑1 (MQ=255)
                                            cGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGaa                                                                        >  2:1274875/1‑140 (MQ=255)
                                            cGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGaa                                                                        >  1:1125317/1‑140 (MQ=255)
                                            cGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGaa                                                                        >  2:611412/1‑140 (MQ=255)
                                                gacgacATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCa                                                                    <  1:552263/140‑1 (MQ=255)
                                                             aCTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTAGAGACCTGAATTCAGGCTGGAGAGGGc                                                       >  1:988719/1‑140 (MQ=255)
                                                              cTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCa                                                      >  1:390932/1‑140 (MQ=255)
                                                              cTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCa                                                      >  1:294683/1‑140 (MQ=255)
                                                               tAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAg                                                     >  1:787032/1‑140 (MQ=255)
                                                                  tgtCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCgg                                                  >  1:827636/1‑140 (MQ=255)
                                                                   gtCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGt                                                 >  2:48412/1‑140 (MQ=255)
                                                                    tCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTc                                                >  2:1191993/1‑140 (MQ=255)
                                                                    tCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTc                                                >  1:524298/1‑140 (MQ=255)
                                                                    tCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTc                                                >  1:1003201/1‑140 (MQ=255)
                                                                      gCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGt                                                 <  2:138119/137‑1 (MQ=255)
                                                                      gCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGt                                                 >  1:138119/1‑137 (MQ=255)
                                                                      gCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAg                                              >  1:1403571/1‑140 (MQ=255)
                                                                        cAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGca                                            <  2:237309/140‑1 (MQ=255)
                                                                        cAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGca                                            >  1:508554/1‑140 (MQ=255)
                                                                          gAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCa                                               >  1:722210/1‑135 (MQ=255)
                                                                          gAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCa                                               <  2:722210/135‑1 (MQ=255)
                                                                          gAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGcacc                                          <  2:294683/140‑1 (MQ=255)
                                                                             tCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACcaca                                       <  2:642772/140‑1 (MQ=255)
                                                                             tCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACcaca                                       <  2:827636/140‑1 (MQ=255)
                                                                               ccGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAAt                                     <  2:1003201/140‑1 (MQ=255)
                                                                                cGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATg                                    >  2:36574/1‑140 (MQ=255)
                                                                                          cacaTGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAAtgc                          >  2:498327/1‑140 (MQ=255)
                                                                                             aTGCTGACGACTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAAtgctgc                       >  2:572654/1‑140 (MQ=255)
                                                                                                  gacgacTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGata                  >  1:453594/1‑140 (MQ=255)
                                                                                                   gcgacTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGatat                 <  2:790445/139‑1 (MQ=255)
                                                                                                    cgacTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGatata                >  1:50966/1‑140 (MQ=255)
                                                                                                      acTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATg              >  1:531159/1‑140 (MQ=255)
                                                                                                       cTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATgg             >  1:876526/1‑140 (MQ=255)
                                                                                                       cTCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATgg             >  1:820501/1‑140 (MQ=255)
                                                                                                        tCATTTTCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGc            <  2:508554/140‑1 (MQ=255)
                                                                                                             ttCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACAcc       <  1:498327/140‑1 (MQ=255)
                                                                                                             ttCGCCATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACAcc       <  1:48412/140‑1 (MQ=255)
                                                                                                                  cATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGtt  <  2:390932/140‑1 (MQ=255)
                                                                                                                  cATAAAACTCGGCGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGtt  >  1:1392822/1‑140 (MQ=255)
                                                                                                                              |                                                                                                                               
CACAATCTCAAATACGCGCGACGGCACCAGATAGTTGAGGAATACGCCGACGACATACACAACTAGTGTCGCCAGAATCCCGGCATACGGCACATGCTGACGACTCATTTTCGCCATAAAACTCGGTGCGGAACCGCCCATCGCCATTGAGCGCAGAATACGTCCGGTGCAGTACAGACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTT  >  NC_000913/1524838‑1525091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A