Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 191 83.5 2830284 99.3% 2810472 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,037,675 (C)5→4 coding (432/1734 nt) recJ ← ssDNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,037,6710C.100.0% 214.8 / NA 50coding (436/1734 nt)recJssDNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (26/24);  total (26/24)

CTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTG  >  NC_000913/3037536‑3037804
                                                                                                                                       |                                                                                                                                     
cTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAAccccg                                                                                                                                   <  2:836803/140‑2 (MQ=255)
 tGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGCTAATGATCGCTTCCGCTGCGGGCAATGTGTCGCCTGGCAAATGGTGAGCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAAccccgc                                                                                                                                  >  2:935236/1‑138 (MQ=255)
    cAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCCTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATg                                                                                                                              <  2:996884/140‑1 (MQ=255)
      gTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCTAACGAGCGAGCGTGCTCAA‑CCCCGCATggg                                                                                                                            <  1:665878/140‑1 (MQ=255)
        gATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGggag                                                                                                                          >  2:424973/1‑140 (MQ=255)
             cGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAAt                                                                                                                     >  1:374095/1‑140 (MQ=255)
              gACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATa                                                                                                                    <  1:1250127/140‑1 (MQ=255)
                    aaaTTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTa                                                                                                              <  1:619971/140‑1 (MQ=255)
                      aTTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATc                                                                                                            <  1:1390293/140‑1 (MQ=255)
                           aGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCg                                                                                                       <  1:923187/140‑1 (MQ=255)
                             tCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTg                                                                                                     <  2:444387/140‑1 (MQ=255)
                               gcgcAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGAc                                                                                                   >  2:967191/1‑140 (MQ=255)
                                          ggTTAATGATCGCTTCCGCTGCGTGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTgcg                                                                                        <  1:351076/140‑1 (MQ=255)
                                               aTGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCa                                                                                   >  1:1060446/1‑140 (MQ=255)
                                               aTGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCa                                                                                   >  1:506650/1‑140 (MQ=255)
                                                    cGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGc                                                                              <  2:388560/140‑1 (MQ=255)
                                                     gCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCGAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGc                                                                              <  1:805645/139‑1 (MQ=255)
                                                     gCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCGAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGc                                                                              >  2:805645/1‑139 (MQ=255)
                                                      cTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCAt                                                                            >  2:1076154/1‑140 (MQ=255)
                                                        tCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATgg                                                                          <  2:299514/140‑1 (MQ=255)
                                                          cGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCa                                                                             <  1:1161842/135‑1 (MQ=255)
                                                          cGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCa                                                                             >  2:1161842/1‑135 (MQ=255)
                                                            cTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCt                                                                      >  2:788255/1‑140 (MQ=255)
                                                              gCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGa                                                                    <  2:348689/140‑1 (MQ=255)
                                                                 ggTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCg                                                                 >  2:844410/1‑140 (MQ=255)
                                                                 ggTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCg                                                                 >  2:1238530/1‑140 (MQ=255)
                                                                 ggTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCg                                                                 >  2:431313/1‑140 (MQ=255)
                                                                 ggTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCg                                                                 >  2:233712/1‑140 (MQ=255)
                                                                           cGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCg                                                       <  1:1076154/140‑1 (MQ=255)
                                                                            gCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCgg                                                      >  2:960782/1‑140 (MQ=255)
                                                                            gCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCgg                                                      >  1:1016991/1‑140 (MQ=255)
                                                                               tGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCt                                                   <  2:689865/140‑1 (MQ=255)
                                                                                 gCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTa                                                 <  1:233712/140‑1 (MQ=255)
                                                                                      tGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCg                                            >  2:941607/1‑140 (MQ=255)
                                                                                           aTCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGCGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAAcc                                       >  1:126653/1‑140 (MQ=255)
                                                                                                tAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCtt                                  >  1:1402795/1‑140 (MQ=255)
                                                                                                tAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCtt                                  >  2:510769/1‑140 (MQ=255)
                                                                                                  aCAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCg                                >  2:691075/1‑140 (MQ=255)
                                                                                                     aTAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGaaa                             <  2:1060446/140‑1 (MQ=255)
                                                                                                     aTAACCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGaaa                             <  2:506650/140‑1 (MQ=255)
                                                                                                        aCCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACgg                          >  2:726309/1‑140 (MQ=255)
                                                                                                        aCCGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACgg                          >  2:406219/1‑140 (MQ=255)
                                                                                                          cGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGtt                        <  1:287882/140‑1 (MQ=255)
                                                                                                          cGGGATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGtt                        <  1:726309/140‑1 (MQ=255)
                                                                                                             gATGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTgg                     >  2:889540/1‑140 (MQ=255)
                                                                                                              aTGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGt                    <  2:497407/140‑1 (MQ=255)
                                                                                                              aTGCCCAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGt                    >  1:386589/1‑140 (MQ=255)
                                                                                                                 cccAACGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTAcc                 <  1:788255/140‑1 (MQ=255)
                                                                                                                    aaCGAGCGAGCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAgg              <  1:928239/140‑1 (MQ=255)
                                                                                                                      cgagcgggCGTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTa            <  1:465239/140‑1 (MQ=255)
                                                                                                                          cgagcgTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCg        >  2:865697/1‑140 (MQ=255)
                                                                                                                            agcgTGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGat      <  2:570929/140‑1 (MQ=255)
                                                                                                                                tGCTCAA‑CCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATtg  >  2:1359605/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                     
CTGCCAGTGATTTCGACGGGAAATTACAGTCGCGCAAGTTAGGGTTAATGATCGCTTCCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTG  >  NC_000913/3037536‑3037804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A