Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 191 83.5 2830284 99.3% 2810472 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,231,336 A→T intergenic (+97/‑329) ygjK → / → fadH glycoside hydrolase/2,4‑dienoyl‑CoA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,231,3360AT100.0% 146.0 / NA 42intergenic (+97/‑329)ygjK/fadHglycoside hydrolase/2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (23/19);  total (23/19)

ATATGTTGTATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGT  >  NC_000913/3231205‑3231393
                                                                                                                                   |                                                         
atatGTTGTATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACcg                                                   <  1:628340/140‑1 (MQ=255)
    gttgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGcc                                               >  2:780458/1‑140 (MQ=255)
    gttgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGcc                                               <  1:655468/140‑1 (MQ=255)
    gttgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGCGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGcc                                               >  2:711036/1‑140 (MQ=255)
     ttgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCa                                              <  1:780458/140‑1 (MQ=255)
      tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  2:1055874/1‑140 (MQ=255)
      tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  2:817715/1‑140 (MQ=255)
      tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  1:906699/1‑140 (MQ=255)
      tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  1:900893/1‑140 (MQ=255)
      tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  1:832234/1‑140 (MQ=255)
      tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  2:757812/1‑140 (MQ=255)
      tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  1:413143/1‑140 (MQ=255)
      tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  1:415681/1‑140 (MQ=255)
      tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  2:641231/1‑140 (MQ=255)
      tgtATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGCCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCAc                                             >  2:924039/1‑140 (MQ=255)
        tataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTg                                           >  2:1040404/1‑140 (MQ=255)
        tataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTg                                           >  2:154180/1‑140 (MQ=255)
         ataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACcgcg                                                 <  2:552275/133‑1 (MQ=255)
         ataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACcgcg                                                 >  1:552275/1‑133 (MQ=255)
         ataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTgg                                          >  1:894578/1‑140 (MQ=255)
         ataACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTgg                                          >  2:544678/1‑140 (MQ=255)
             cGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCAcc                                                    <  1:171248/126‑1 (MQ=255)
             cGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCAcc                                                    >  2:171248/1‑126 (MQ=255)
             cGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACcg                                                   >  1:283677/1‑127 (MQ=255)
             cGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACcg                                                   <  2:283677/127‑1 (MQ=255)
                 tttttCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATgcg                                  <  1:154180/140‑1 (MQ=255)
                  ttttCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACTCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATgccg                                 >  1:104884/1‑138 (MQ=255)
                  ttttCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATgcgg                                 >  2:354039/1‑140 (MQ=255)
                      ccGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg                             <  2:1410442/140‑1 (MQ=255)
                      ccGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg                             <  2:344532/140‑1 (MQ=255)
                      ccGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTg                             <  1:357463/140‑1 (MQ=255)
                         tAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGAc                          >  2:773268/1‑140 (MQ=255)
                           aGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGc                        <  1:1055874/140‑1 (MQ=255)
                                taatTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTAt                   <  1:366957/140‑1 (MQ=255)
                                 aatTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATc                  <  1:757812/140‑1 (MQ=255)
                                    ttGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGc               <  2:1224726/140‑1 (MQ=255)
                                    ttGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGc               <  1:641231/140‑1 (MQ=255)
                                              tgGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACAtgtg     <  1:924039/140‑1 (MQ=255)
                                               ggCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACAtgtgc    >  2:1274568/1‑139 (MQ=255)
                                                gCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACAtgtgca   <  2:385367/140‑3 (MQ=255)
                                                gCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACAtgtgca   <  1:1144699/140‑3 (MQ=255)
                                                 cGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTCGCATCCGGCATCTGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACAtgtgcaa  <  1:1040404/140‑4 (MQ=255)
                                                                                                                                   |                                                         
ATATGTTGTATAACGATTTTTTCCGTAAGCAATAATTTGCAACTGCTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGT  >  NC_000913/3231205‑3231393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A