Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,241,613	(A)_7→8	coding (132/552 nt)	ygjV ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,241,606	1	.	A	80.0%	218.5 / 44.9	75	coding (139/552 nt)	ygjV	inner membrane protein
Reads supporting (aligned to +/- strand): ref base . (9/6); new base A (30/30); total (39/36)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCG  >  NC_000913/3241475‑3241739

cTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAGGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑aaaaaaaa                                                                                                                               >  1:840755/1‑139 (MQ=255)

cTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑aaaaaaaa                                                                                                                               >  1:115875/1‑139 (MQ=255)

 ttCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑AAAAAAATg                                                                                                                              >  1:1068719/1‑140 (MQ=255)

   cgcgAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑aaaaaaaatgt                                                                                                                            >  2:1210329/1‑136 (MQ=255)

    gcgAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑aaaaaaaatgta                                                                                                                           <  2:600636/140‑6 (MQ=255)

    gcgAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑aaaaaaaatgta                                                                                                                           >  2:670983/1‑135 (MQ=255)

    gcgAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑AAAAAAATGTAc                                                                                                                           >  1:1030973/1‑140 (MQ=255)

     cgAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTAc                                                                                                                           >  1:1088355/1‑140 (MQ=255)

         gCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCa                                                                                                                       >  1:680093/1‑140 (MQ=255)

             aTTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAGGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATaa                                                                                                                   <  2:840755/140‑1 (MQ=255)

              ttCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAAc                                                                                                                  >  2:1359271/1‑140 (MQ=255)

               tCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACg                                                                                                                 <  2:680093/140‑1 (MQ=255)

                ccGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACgg                                                                                                                <  1:838320/140‑1 (MQ=255)

                  gCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCTCTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGgcg                                                                                                              <  2:1134747/140‑1 (MQ=255)

                      gTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCtat                                                                                                          >  2:617860/1‑140 (MQ=255)

                           cacaATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGAcc                                                                                                     >  2:439744/1‑140 (MQ=255)

                           cacaATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGAcc                                                                                                     <  2:85309/140‑1 (MQ=255)

                               aTAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAg                                                                                                <  1:1310866/140‑1 (MQ=255)

                                tAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAg                                                                                                <  2:611853/140‑1 (MQ=255)

                                tAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAg                                                                                                <  1:513613/140‑1 (MQ=255)

                                tAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTAGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAg                                                                                                >  2:1303434/1‑140 (MQ=255)

                                 aaaaaTCGCCATTACCCATAAGCTGCGCGTGCGTTAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAgct                                                                                              >  1:1147397/1‑140 (MQ=255)

                                        gCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCtttt                                                                                        >  1:746351/1‑140 (MQ=255)

                                          cATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTg                                                                                      >  1:509692/1‑140 (MQ=255)

                                              aCCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCg                                                                                 <  2:1068719/140‑1 (MQ=255)

                                                ccATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGAAAAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCg                                                                                 <  1:915985/140‑1 (MQ=255)

                                                  aTAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGt                                                                             >  2:1186463/1‑140 (MQ=255)

                                                    aaGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTc                                                                           >  2:1024900/1‑140 (MQ=255)

                                                       cTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTc                                                                        >  1:114440/1‑140 (MQ=255)

                                                          cgcgTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCAc                                                                      >  2:232097/1‑140 (MQ=255)

                                                          cgcgTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCAc                                                                      >  2:1350178/1‑140 (MQ=255)

                                                          cgcgTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCAc                                                                      >  1:1000863/1‑140 (MQ=255)

                                                          cgcgTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCAc                                                                      >  2:563406/1‑140 (MQ=255)

                                                          cgcgTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCAc                                                                      >  1:1164274/1‑140 (MQ=255)

                                                          cgcgTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCAc                                                                      >  1:131449/1‑140 (MQ=255)

                                                          cgcgTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACg                                                                     >  2:1152098/1‑140 (MQ=255)

                                                            cgtgcgtAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGa                                                                    >  1:9168/1‑140 (MQ=255)

                                                              tgcgtAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGa                                                                    <  2:878139/138‑1 (MQ=255)

                                                              tgcgtAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGa                                                                    >  1:878139/1‑138 (MQ=255)

                                                              tgcgtAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGAtt                                                                  <  1:1132853/140‑1 (MQ=255)

                                                              tgcgtAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGAtt                                                                  >  2:671542/1‑140 (MQ=255)

                                                                 gtAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGaa                                                               <  2:131449/140‑1 (MQ=255)

                                                                 gtAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGaa                                                               <  1:1359271/140‑1 (MQ=255)

                                                                         aTCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAAtgttg                                                       <  2:1000863/140‑1 (MQ=255)

                                                                         aTCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAAtgttg                                                       >  1:929239/1‑140 (MQ=255)

                                                                         aTCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAAtgttg                                                       >  1:306205/1‑140 (MQ=255)

                                                                           cAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTtgtg                                                     >  2:70065/1‑140 (MQ=255)

                                                                                 aCGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCaa                                              >  1:677954/1‑140 (MQ=255)

                                                                                     aTGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAg                                          >  1:1309566/1‑140 (MQ=255)

                                                                                      tGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAg                                          <  1:439744/140‑1 (MQ=255)

                                                                                      tGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAg                                          >  1:556701/1‑140 (MQ=255)

                                                                                       ggCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGa                                         <  1:617860/140‑1 (MQ=255)

                                                                                        gCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGaa                                        >  2:966568/1‑140 (MQ=255)

                                                                                        gCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGaa                                        >  1:1039364/1‑140 (MQ=255)

                                                                                        gCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGaa                                        >  1:1043349/1‑140 (MQ=255)

                                                                                        gCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCCATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGaa                                        >  1:739554/1‑140 (MQ=255)

                                                                                        gCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGaaa                                       <  1:1304245/140‑1 (MQ=255)

                                                                                        gCATTAAGGATGGCACTGGCACCAGCGGGATAGGCGCCCAGAAGA‑AAAAAAATGAACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGaa                                        >  2:504442/1‑140 (MQ=255)

                                                                                               ggATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGAt                                 <  2:960614/140‑1 (MQ=255)

                                                                                                 aTGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGa                               <  2:9168/140‑1 (MQ=255)

                                                                                                 aTGGCACTGGCACCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGa                               <  1:670983/140‑1 (MQ=255)

                                                                                                   ggcactggcacCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGaca                             >  2:748227/1‑140 (MQ=255)

                                                                                                   ggcactggcacCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGaca                             >  1:516693/1‑140 (MQ=255)

                                                                                                   ggcactggcacCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGaca                             >  2:579458/1‑140 (MQ=255)

                                                                                                      actggcacCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACAccc                          <  2:516693/140‑1 (MQ=255)

                                                                                                      actggcacCAGCGGGATAGGTGCCCAGAAG‑‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCa                         <  1:1024900/140‑1 (MQ=255)

                                                                                                      actggcacCAGCGGGATAGGTGCCCAGAAG‑‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCa                         <  2:114440/140‑1 (MQ=255)

                                                                                                       ctggcacCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCa                         <  1:675502/140‑1 (MQ=255)

                                                                                                       ctggcacCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCa                         <  1:67744/140‑1 (MQ=255)

                                                                                                       ctggcacCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCa                         <  2:746351/140‑1 (MQ=255)

                                                                                                       ctggcacCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCa                         <  2:929239/140‑1 (MQ=255)

                                                                                                           cacCAGCGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGcc                     <  2:1088355/140‑1 (MQ=255)

                                                                                                                 cGGGATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGgc               <  1:1210329/140‑1 (MQ=255)

                                                                                                                    gATAGGTGCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGgccag            >  1:1404897/1‑140 (MQ=255)

                                                                                                                          tGCCCAGAAGA‑AAAAAAATGTACGCCAATAATGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATa      >  1:37659/1‑140 (MQ=255)

                                                                                                                           gCCCAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATAc     <  2:556701/140‑1 (MQ=255)

                                                                                                                            cccAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATAcg    <  1:748227/140‑1 (MQ=255)

                                                                                                                            cccAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATAcg    <  2:1043349/140‑1 (MQ=255)

                                                                                                                            cccAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATAcg    <  2:1147440/140‑1 (MQ=255)

                                                                                                                            cccAGAAGA‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGCCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATAcg    <  1:1303434/140‑1 (MQ=255)

                                                                                                                             ccAGAAG‑‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATAcgcg  <  2:1309566/140‑1 (MQ=255)

CTTCGCGAGGCCAATTCCGCCAGTCAGCACAATAAAAATCGCCATTACCCATAAGCTGCGCGTGCGTAAGGTAATCAATGTACGAATGGCATTAAGGATGGCACTGGCACCAGCGGGATAGGTGCCCAGAAG‑‑AAAAAAATGTACGCCAATAACGGCGCTATAGACCGAAAGCTGCTTTTTGAAGCGACGTTCGTCACGATTGAAAAATGTTGTGATACCAATCAGAAAGGCGATGACACCCACGCCCTGGGCCAGCCAATACGCG  >  NC_000913/3241475‑3241739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A