Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,454,255	A→G	S382P (TCG→CCG)	gspA ←	Type II secretion system protein GspA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,454,255	0	A	G	98.7%	278.6 / ‑3.5	77	S382P (TCG→CCG)	gspA	Type II secretion system protein GspA
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (47/29); minor base C (1/0); total (48/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

CTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGACTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCAGGGCAAATTCTGA  >  NC_000913/3454120‑3454391

cTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCa                                                                                                                                      <  2:610741/140‑1 (MQ=255)

 tGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGCCCATACCGGCTCaa                                                                                                                                     >  2:793243/1‑140 (MQ=255)

      aaaTATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCAt                                                                                                                                <  2:880855/140‑1 (MQ=255)

        atatGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCAttt                                                                                                                              >  2:54110/1‑140 (MQ=255)

        atatGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCAttt                                                                                                                              <  2:1185915/140‑1 (MQ=255)

           tGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTAtg                                                                                                                           >  2:93935/1‑140 (MQ=255)

              aaTGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATgtgt                                                                                                                        <  2:1182466/140‑1 (MQ=255)

                  cgcgGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGc                                                                                                                    >  1:269842/1‑140 (MQ=255)

                   gcgGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCg                                                                                                                   <  1:54110/140‑1 (MQ=255)

                     ggTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGt                                                                                                                  <  1:791783/139‑1 (MQ=255)

                     ggTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGt                                                                                                                  >  2:791783/1‑139 (MQ=255)

                                 tttCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCGGCGTCCACGTTTGCTGa                                                                                                     >  2:1258548/1‑140 (MQ=255)

                                 tttCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGa                                                                                                     >  2:1301729/1‑140 (MQ=255)

                                 tttCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGa                                                                                                     >  1:34893/1‑140 (MQ=255)

                                 tttCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGa                                                                                                     >  2:511901/1‑140 (MQ=255)

                                 tttCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGa                                                                                                     >  2:852087/1‑140 (MQ=255)

                                    ccagccagAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCt                                                                                                  <  1:903383/140‑1 (MQ=255)

                                       gccagAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGTTGACCTACg                                                                                               <  1:152678/140‑1 (MQ=255)

                                       gccagAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACg                                                                                               >  2:121138/1‑140 (MQ=255)

                                        ccagAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGa                                                                                              <  1:378098/140‑1 (MQ=255)

                                         cagAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAg                                                                                             <  1:1258548/140‑1 (MQ=255)

                                            agaATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGAc                                                                                          >  2:161986/1‑140 (MQ=255)

                                              aaTCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACAt                                                                                        <  1:511901/140‑1 (MQ=255)

                                                   ctTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACg                                                                                   <  1:1089879/140‑1 (MQ=255)

                                                        tCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGc                                                                              >  2:1041322/1‑140 (MQ=255)

                                                         cGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGcc                                                                             >  1:165968/1‑140 (MQ=255)

                                                           ctgctATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTc                                                                           >  1:831111/1‑140 (MQ=255)

                                                                aTCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGa                                                                      >  2:1342574/1‑140 (MQ=255)

                                                                    cgcgTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGAcgcg                                                                  <  1:1041322/140‑1 (MQ=255)

                                                                     gcgTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGa                                                                 <  1:1342574/140‑1 (MQ=255)

                                                                     gcgTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGa                                                                 <  1:161986/140‑1 (MQ=255)

                                                                        tAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACg                                                              >  1:244529/1‑140 (MQ=255)

                                                                          aTGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGAc                                                            <  2:69784/140‑1 (MQ=255)

                                                                           tGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGa                                                                 <  1:639671/134‑1 (MQ=255)

                                                                           tGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGa                                                                 >  2:639671/1‑134 (MQ=255)

                                                                             gTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACcac                                                         >  2:144526/1‑140 (MQ=255)

                                                                               aCTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCacaa                                                       >  2:1078262/1‑140 (MQ=255)

                                                                                 tctcCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCacaaca                                                     >  1:1278828/1‑140 (MQ=255)

                                                                                  ctcCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAg                                                    >  1:1041960/1‑140 (MQ=255)

                                                                                  ctcCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAg                                                    >  2:1163758/1‑140 (MQ=255)

                                                                                   tcCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAgg                                                   >  1:1068316/1‑140 (MQ=255)

                                                                                   tcCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAgg                                                   >  2:703128/1‑140 (MQ=255)

                                                                                   tcCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAgg                                                   >  2:922484/1‑140 (MQ=255)

                                                                                   tcCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAgg                                                   >  2:1408311/1‑140 (MQ=255)

                                                                                   tcCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAgg                                                   >  2:101181/1‑140 (MQ=255)

                                                                                   tcCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGCCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAgg                                                   >  1:593272/1‑140 (MQ=255)

                                                                                     cccTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCa                                                 >  2:927099/1‑140 (MQ=255)

                                                                                     cccTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCa                                                 >  1:750133/1‑140 (MQ=255)

                                                                                     cccTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCa                                                 >  2:92339/1‑140 (MQ=255)

                                                                                             gCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTg                                         >  2:1303393/1‑140 (MQ=255)

                                                                                             gCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTg                                         >  2:349597/1‑140 (MQ=255)

                                                                                                 cATTTTCCACAACAGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTccc                                     <  1:1202188/140‑1 (MQ=255)

                                                                                                           aacaGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAg                           >  1:155812/1‑140 (MQ=255)

                                                                                                           aacaGAAGATAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAg                           <  1:922484/140‑1 (MQ=255)

                                                                                                                aagaTAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGTCCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAgcgatg                      >  2:264860/1‑140 (MQ=255)

                                                                                                                aagaTAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAgcgatg                      <  1:51982/140‑1 (MQ=255)

                                                                                                                aagaTAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAgcgatg                      <  2:155812/140‑1 (MQ=255)

                                                                                                                aagaTAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAgcgatg                      <  2:750133/140‑1 (MQ=255)

                                                                                                                aagaTAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAgcgatg                      >  2:514008/1‑140 (MQ=255)

                                                                                                                aagaTAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAgcgatg                      >  2:58932/1‑140 (MQ=255)

                                                                                                                  gaTAATCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAgcgatgcg                    >  1:1402266/1‑140 (MQ=255)

                                                                                                                     aaTCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATc                 >  2:216748/1‑140 (MQ=255)

                                                                                                                      aTCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATcc                <  1:144526/140‑1 (MQ=255)

                                                                                                                       tCACCCGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCa               >  1:417585/1‑140 (MQ=255)

                                                                                                                          cccGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCAggg            >  1:84368/1‑140 (MQ=255)

                                                                                                                          cccGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCAggg            >  1:1158229/1‑140 (MQ=255)

                                                                                                                          cccGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCAggg            >  2:1391990/1‑140 (MQ=255)

                                                                                                                          cccGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCAggg            >  1:180065/1‑140 (MQ=255)

                                                                                                                           ccGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCAGGGc           <  2:34893/140‑1 (MQ=255)

                                                                                                                           ccGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCAGGGc           <  2:269842/140‑1 (MQ=255)

                                                                                                                           ccGTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCAGGGc           <  1:703128/140‑1 (MQ=255)

                                                                                                                             gTCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCAGGGCaa         >  2:687832/1‑140 (MQ=255)

                                                                                                                              tCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCAGGGCaaa        >  1:415513/1‑140 (MQ=255)

                                                                                                                              tCCATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCAGGGCaaa        >  1:100537/1‑140 (MQ=255)

                                                                                                                               ccATACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCAGGGCAAAt       <  1:92339/140‑1 (MQ=255)

                                                                                                                                 aTACCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCAGGGCAAATTc     <  2:1068316/140‑1 (MQ=255)

                                                                                                                                   aCCGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCAGGGCAAATTCTg   <  1:927099/140‑1 (MQ=255)

                                                                                                                                    ccGGCTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCAGGGCAAATTCTGa  <  1:1408311/140‑1 (MQ=255)

CTGTCGAAATATGCAATGCGCGGTTTAACATCGTTTCCAGCCAGAGAATCTCTTCCTCGCTGCTATCACGCGTAATGGTACTCTCCCCTTCCGGCGACATTTTCCACAACAGAAGATAATCACCCGTCCATACCGACTCAAACCATTTATGTGTCAGCGTCCACGTTTGCTGACCTACGAGGACATCAACGCTAGCCTCACCGACGCGAACGACCACAACAGGCAATTTTTTGTCCCCGACTTTAAGCGATGCGATCCAGGGCAAATTCTGA  >  NC_000913/3454120‑3454391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A