Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,685,631	(C)_6→7	intergenic (‑13/+69)	pdeK ← / ← bcsC	putative c‑di‑GMP phosphodiesterase PdeK/cellulose biosynthesis protein BcsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,685,625	1	.	C	100.0%	216.7 / NA	59	intergenic (‑7/+75)	pdeK/bcsC	putative c‑di‑GMP phosphodiesterase PdeK/cellulose biosynthesis protein BcsC
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (26/33); total (26/33)

GCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCT‑CCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTG  >  NC_000913/3685486‑3685753

gCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGtct                                                                                                                                   >  2:548694/1‑140 (MQ=255)

gCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGAGACTTACGCGCAAATTGtct                                                                                                                                   >  2:1145846/1‑140 (MQ=255)

   tAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCT‑ccc                                                                                                                               >  1:713715/1‑140 (MQ=255)

   tAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCT‑ccc                                                                                                                               >  1:147077/1‑140 (MQ=255)

      ccGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCT‑cccccc                                                                                                                            >  1:611078/1‑140 (MQ=255)

      ccGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCT‑cccccc                                                                                                                            >  1:4343/1‑140 (MQ=255)

           ttATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCT‑cccccccggaa                                                                                                                       >  2:609703/1‑135 (MQ=255)

           ttATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCT‑cccccccggaa                                                                                                                       >  2:1398801/1‑135 (MQ=255)

                   aTTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGa                                                                                                                >  1:754481/1‑140 (MQ=255)

                    ttCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGAt                                                                                                               >  1:397551/1‑140 (MQ=255)

                    ttCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGAt                                                                                                               <  2:1271597/140‑1 (MQ=255)

                     tCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATGTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGAtt                                                                                                              >  2:1265046/1‑140 (MQ=255)

                     tCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGAtt                                                                                                              >  1:54913/1‑140 (MQ=255)

                       tgctgGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTgc                                                                                                            <  1:1327246/140‑1 (MQ=255)

                       tgctgGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTgc                                                                                                            <  1:1329630/140‑1 (MQ=255)

                          tgGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTgccgc                                                                                                         <  2:859945/140‑1 (MQ=255)

                           ggACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTgccgcc                                                                                                        <  2:713715/140‑1 (MQ=255)

                           ggACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTgccgcc                                                                                                        <  1:609703/140‑1 (MQ=255)

                            gACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGccc                                                                                                       <  2:245353/140‑1 (MQ=255)

                              ccAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGa                                                                                                     >  2:180356/1‑140 (MQ=255)

                                           cAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCg                                                                                        >  2:721150/1‑140 (MQ=255)

                                             aaaCGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCa                                                                                      <  1:358466/140‑1 (MQ=255)

                                              aaCGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAg                                                                                     <  2:177080/140‑1 (MQ=255)

                                               aCGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGc                                                                                    <  2:572743/140‑1 (MQ=255)

                                               aCGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGc                                                                                    <  1:1252695/140‑1 (MQ=255)

                                               aCGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGc                                                                                    <  1:1205984/140‑1 (MQ=255)

                                                cGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATAc                                                                                               >  2:105995/1‑128 (MQ=255)

                                                cGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATAc                                                                                               <  1:105995/128‑1 (MQ=255)

                                                cGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCg                                                                                   <  2:754481/140‑1 (MQ=255)

                                                cGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCg                                                                                   <  1:1398801/140‑1 (MQ=255)

                                                    gCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGc                                                                                 <  1:648727/138‑1 (MQ=255)

                                                    gCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGc                                                                                 >  2:648727/1‑138 (MQ=255)

                                                         aaaTAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAaga                                                                          <  2:518837/140‑1 (MQ=255)

                                                          aaTAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGc                                                                                    >  1:582173/1‑129 (MQ=255)

                                                          aaTAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGc                                                                                    <  2:582173/129‑1 (MQ=255)

                                                          aaTAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAagag                                                                         <  2:397551/140‑1 (MQ=255)

                                                          aaTAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAagag                                                                         <  1:180356/140‑1 (MQ=255)

                                                                     acacCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCt                                                              <  2:748383/140‑1 (MQ=255)

                                                                     acacCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCt                                                              <  2:1243374/140‑1 (MQ=255)

                                                                       acCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGa                                                            <  2:364616/140‑1 (MQ=255)

                                                                                   gCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGt                                                <  1:721150/140‑1 (MQ=255)

                                                                                   gCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGt                                                <  1:1320921/140‑1 (MQ=255)

                                                                                      gCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCgg                                             >  2:394033/1‑140 (MQ=255)

                                                                                      gCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCgg                                             >  1:1046697/1‑140 (MQ=255)

                                                                                        caccaTTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCg                                           <  2:616604/140‑1 (MQ=255)

                                                                                        caccaTTACCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCg                                           <  2:31815/140‑1 (MQ=255)

                                                                                         accaTTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGt                                                >  2:354659/1‑134 (MQ=255)

                                                                                         accaTTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGt                                                <  1:354659/134‑1 (MQ=255)

                                                                                              tGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCgg                                             >  1:72839/1‑132 (MQ=255)

                                                                                              tGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCgg                                             <  2:72839/132‑1 (MQ=255)

                                                                                                     tGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCg                              >  1:1370305/1‑140 (MQ=255)

                                                                                                       cTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGc                            >  2:74187/1‑140 (MQ=255)

                                                                                                        ttGATTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCt                           >  2:938755/1‑140 (MQ=255)

                                                                                                           aTTGTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTgcg                        <  1:1265046/140‑1 (MQ=255)

                                                                                                              gTTAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTgcggcg                     <  1:938755/140‑1 (MQ=255)

                                                                                                               ttAACGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGcggcgg                    >  1:367504/1‑140 (MQ=255)

                                                                                                                 aaCGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTa                  >  2:464062/1‑140 (MQ=255)

                                                                                                                 aaCGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTa                  >  1:219431/1‑140 (MQ=255)

                                                                                                                 aaCGAGCGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCAAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTa                  >  1:350466/1‑140 (MQ=255)

                                                                                                                       cGACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCa            >  2:984993/1‑140 (MQ=255)

                                                                                                                        gACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCAt           <  1:1145846/140‑1 (MQ=255)

                                                                                                                        gACTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCAt           <  1:548694/140‑1 (MQ=255)

                                                                                                                         aCTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATg          >  2:1273362/1‑140 (MQ=255)

                                                                                                                         aCTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATg          >  2:136247/1‑140 (MQ=255)

                                                                                                                         aCTTACGCGCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATg          >  2:154936/1‑140 (MQ=255)

                                                                                                                              cgcgCAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCAcc     >  1:509337/1‑140 (MQ=255)

                                                                                                                                 gcAAATTGTCTCCCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTg  >  1:149258/1‑140 (MQ=255)

GCGTAGCCGTGTTATAGCGATTCTGCTGGACCAGATGGAACAGCAAAACGGTGCAAAAAATAAAAACGAACACCAGGACAACGGCTGCCACCATTGCCATCTGCTTGATTGTTAACGAGCGACTTACGCGCAAATTGTCT‑CCCCCCGGAAATCCCAGATTGCCGCCCGAGTATACCCGATCGCAGCGGCATTAAGAGAGGCGCTATCTGAAAACTTACCAGTCGGCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTG  >  NC_000913/3685486‑3685753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A