Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I1 R1 191 83.5 2830284 99.3% 2810472 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,724,669 T→C D217G (GAC→GGC)  glyQ ← glycine‑‑tRNA ligase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,724,6690TC100.0% 234.8 / NA 65D217G (GAC→GGC) glyQglycine‑‑tRNA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/28);  total (37/28)

TACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGTCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGTCTACGCCCTGAATGTAC  >  NC_000913/3724535‑3724803
                                                                                                                                      |                                                                                                                                      
tACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCcaca                                                                                                                                   <  2:1280593/140‑1 (MQ=255)
 aCGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCGGCGGAATTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACAt                                                                                                                                  >  2:1146640/1‑140 (MQ=255)
        tCCAGCAGGTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTa                                                                                                                           <  2:731814/140‑1 (MQ=255)
           agcagGTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTc                                                                                                                        <  2:177582/140‑1 (MQ=255)
                gTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGt                                                                                                                   <  2:335576/140‑1 (MQ=255)
                gTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGt                                                                                                                   >  1:301203/1‑140 (MQ=255)
                 ttGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGtt                                                                                                                  >  2:161823/1‑140 (MQ=255)
                   gAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGt                                                                                                                >  1:365119/1‑140 (MQ=255)
                     aGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTaa                                                                                                              <  1:450460/140‑1 (MQ=255)
                          tgGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTg                                                                                                           >  1:362400/1‑138 (MQ=255)
                          tgGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTg                                                                                                           <  2:362400/138‑1 (MQ=255)
                          tgGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGa                                                                                                         <  2:22765/140‑1 (MQ=255)
                               ggcTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCt                                                                                                    >  1:421321/1‑140 (MQ=255)
                                gcTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTc                                                                                                   >  1:784069/1‑140 (MQ=255)
                                 cTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTcc                                                                                                  >  1:436381/1‑140 (MQ=255)
                                    tCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACt                                                                                               >  2:1017643/1‑140 (MQ=255)
                                         aTACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGtt                                                                                          <  1:232986/140‑1 (MQ=255)
                                         aTACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTACTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGtt                                                                                          <  2:672401/140‑1 (MQ=255)
                                           aCGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCt                                                                                        <  2:436381/140‑1 (MQ=255)
                                             gCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGa                                                                                      >  1:523804/1‑140 (MQ=255)
                                                  tAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGaa                                                                                 >  1:229413/1‑140 (MQ=255)
                                                      cTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACg                                                                             <  2:1205240/140‑1 (MQ=255)
                                                       tGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGt                                                                            >  1:300660/1‑140 (MQ=255)
                                                       tGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGt                                                                            >  1:117173/1‑140 (MQ=255)
                                                           agcggcagcggATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGcc                                                                        >  2:3661/1‑140 (MQ=255)
                                                           agcggcagcggATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGcc                                                                        <  2:899078/140‑1 (MQ=255)
                                                             cggcagcggATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGt                                                                      >  1:106097/1‑140 (MQ=255)
                                                                cagcggATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAgg                                                                   <  2:181379/140‑1 (MQ=255)
                                                                                       gctgctGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCg                                            >  1:965679/1‑140 (MQ=255)
                                                                                         tgctgcGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCt                                          <  1:630920/140‑1 (MQ=255)
                                                                                               gcTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGat                                    <  2:915814/140‑2 (MQ=255)
                                                                                               gcTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGAc                                    <  1:1313762/140‑1 (MQ=255)
                                                                                                cttcttTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGAcc                                   <  1:161823/140‑1 (MQ=255)
                                                                                                  tctttctCGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAg                                 >  2:169043/1‑140 (MQ=255)
                                                                                                      tctcGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCg                             >  1:181612/1‑140 (MQ=255)
                                                                                                      tctcGTACTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCg                             <  1:3661/140‑1 (MQ=255)
                                                                                                            aCTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACg                       <  2:528854/140‑1 (MQ=255)
                                                                                                             cTGCTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGc                      >  2:1406867/1‑140 (MQ=255)
                                                                                                                cTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCGGACCAGGTCGTAAACGCTGt                   >  2:1193572/1‑140 (MQ=255)
                                                                                                                cTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGa                                     <  1:803281/122‑1 (MQ=255)
                                                                                                                cTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGa                                     >  2:803281/1‑122 (MQ=255)
                                                                                                                cTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCa                                  >  2:573627/1‑125 (MQ=255)
                                                                                                                cTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCa                                  <  1:573627/125‑1 (MQ=255)
                                                                                                                cTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACg                       >  2:1222198/1‑136 (MQ=255)
                                                                                                                cTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACg                       <  1:1222198/136‑1 (MQ=255)
                                                                                                                cTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGc                      >  2:1346745/1‑137 (MQ=255)
                                                                                                                cTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGc                      <  1:1346745/137‑1 (MQ=255)
                                                                                                                cTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGt                   >  2:826224/1‑140 (MQ=255)
                                                                                                                cTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGt                   >  1:1010002/1‑140 (MQ=255)
                                                                                                                cTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGt                   >  1:1363317/1‑140 (MQ=255)
                                                                                                                cTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGt                   >  1:674241/1‑140 (MQ=255)
                                                                                                                cTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGt                   >  2:1255944/1‑140 (MQ=255)
                                                                                                                cTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCCCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGt                   >  1:720306/1‑140 (MQ=255)
                                                                                                                cTCGAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTACTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGt                   >  1:1247959/1‑140 (MQ=255)
                                                                                                                   gAAGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGTCTa                <  1:1255944/140‑1 (MQ=255)
                                                                                                                    aaGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGTCTAc               >  1:1045265/1‑140 (MQ=255)
                                                                                                                     aGCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGTCTACg              >  2:201386/1‑140 (MQ=255)
                                                                                                                      gCAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGTCTACGc             <  2:523804/140‑1 (MQ=255)
                                                                                                                       cAGGTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGACCACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGTCTACGcc            <  2:784069/140‑1 (MQ=255)
                                                                                                                          gTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGTCTACGCCCTg         >  1:819400/1‑140 (MQ=255)
                                                                                                                          gTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGTCTACGCCCTg         >  1:448636/1‑140 (MQ=255)
                                                                                                                          gTGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGTCTACGCCCTg         >  2:356248/1‑140 (MQ=255)
                                                                                                                           tGAACAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGTCTACGCCCTGa        <  1:1017643/140‑1 (MQ=255)
                                                                                                                               cAGGAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGTCTACGCCCTGAATGt    >  2:878331/1‑140 (MQ=255)
                                                                                                                                aGGAAGCCCACATCCGCGTATGCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTTCCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAGCGCTGTCTACGCCCTGAATGTa   <  2:301203/140‑1 (MQ=255)
                                                                                                                                 ggAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGTCTACACCCTGAATGTAc  <  2:117173/140‑1 (MQ=255)
                                                                                                                                 ggAAGCCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGCGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGTCTACGCCCTGAATGTAc  >  1:372075/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                      
TACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTCAGAATACGCTCGTAGGCTGGCAGCGGCAGCGGATTTTCCAGCGCCAGCAGCTGCTGCGCTTCTTTCTCGTACTGCTCGAAGCAGGTGAACAGGAAGTCCACATCCGCGTATTCGAAGTTGTAAGTGGACTGCTCCACTTCGTTCTGATGGAACACGTCGCCGTAGGTGGTTTTACCCAGCGGGCCGTCGCTCCAGACCAGGTCGTAAACGCTGTCTACGCCCTGAATGTAC  >  NC_000913/3724535‑3724803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A