Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,163,650	(A)_6→5	coding (12/1845 nt)	btuB →	cobalamin/cobinamide outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,163,645	0	A	.	100.0%	352.7 / NA	81	coding (7/1845 nt)	btuB	cobalamin/cobinamide outer membrane transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (43/38); total (43/38)

TCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCA  >  NC_000913/4163516‑4163777

tctTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCg                                                                                                                           <  2:1353901/140‑1 (MQ=255)

  ttAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCgct                                                                                                                         >  2:744559/1‑140 (MQ=255)

  ttAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCgct                                                                                                                         >  2:1355580/1‑140 (MQ=255)

  ttAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCgct                                                                                                                         >  2:481139/1‑140 (MQ=255)

      tATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGctgctg                                                                                                                     <  1:211246/140‑1 (MQ=255)

      tATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGctgctg                                                                                                                     >  2:306089/1‑140 (MQ=255)

       aTCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGa                                                                                                                    <  2:438917/140‑1 (MQ=255)

         cTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACg                                                                                                                  <  1:176676/140‑1 (MQ=255)

          ttAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACgg                                                                                                                 >  2:144143/1‑140 (MQ=255)

          ttAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACgg                                                                                                                 >  1:1034824/1‑140 (MQ=255)

            aGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACg                                                                                                                  <  2:624388/137‑1 (MQ=255)

            aGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACg                                                                                                                  >  1:624388/1‑137 (MQ=255)

            aGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCg                                                                                                               >  1:1248206/1‑140 (MQ=255)

            aGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCg                                                                                                               >  1:543233/1‑140 (MQ=255)

                aCCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGtt                                                                                                           >  1:288813/1‑140 (MQ=255)

                aCCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGtt                                                                                                           >  2:1125397/1‑140 (MQ=255)

                  cccTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTcc                                                                                                         >  1:190400/1‑140 (MQ=255)

                  cccTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTcc                                                                                                         >  1:507667/1‑140 (MQ=255)

                   ccTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCg                                                                                                        >  2:1069883/1‑140 (MQ=255)

                    cTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGt                                                                                                       >  2:431081/1‑140 (MQ=255)

                    cTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGt                                                                                                       >  2:118603/1‑140 (MQ=255)

                    cTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGt                                                                                                       >  1:988621/1‑140 (MQ=255)

                       cAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCAc                                                                                                    >  1:743672/1‑140 (MQ=255)

                       cAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGAAGGCGTGTTCCGTCAc                                                                                                    >  1:114602/1‑140 (MQ=255)

                        aaGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACg                                                                                                   <  2:1034824/140‑1 (MQ=255)

                         aGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACgg                                                                                                  <  2:543233/140‑1 (MQ=255)

                         aGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACgg                                                                                                  <  1:306089/140‑1 (MQ=255)

                             cGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCAtt                                                                                              <  2:466366/140‑1 (MQ=255)

                               aaGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCAtttt                                                                                            >  1:607244/1‑140 (MQ=255)

                               aaGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCAtttt                                                                                            >  1:483966/1‑140 (MQ=255)

                                   ccTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGc                                                                                        <  1:1252091/140‑1 (MQ=255)

                                    cTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCt                                                                                       <  1:1058782/140‑1 (MQ=255)

                                    cTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCt                                                                                       <  1:184175/140‑1 (MQ=255)

                                     tGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCtt                                                                                      <  2:483966/140‑1 (MQ=255)

                                        cGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTggg                                                                                   <  1:1355580/140‑1 (MQ=255)

                                          gCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGca                                                                                 <  1:291713/140‑1 (MQ=255)

                                          gCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGca                                                                                 <  1:838021/140‑1 (MQ=255)

                                           ccAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGcac                                                                                <  1:1412699/140‑1 (MQ=255)

                                            cAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGcaca                                                                               >  2:280572/1‑140 (MQ=255)

                                                 tCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATa                                                                          <  1:429186/140‑1 (MQ=255)

                                                    cATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCa                                                                       >  2:1411994/1‑140 (MQ=255)

                                                     aTCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAg                                                                      <  1:144143/140‑1 (MQ=255)

                                                       cTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGcc                                                                    <  2:684293/140‑1 (MQ=255)

                                                         ggTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCg                                                                  >  2:1281466/1‑140 (MQ=255)

                                                           ttCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGca                                                                                 >  1:613761/1‑123 (MQ=255)

                                                           ttCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGca                                                                                 <  2:613761/123‑1 (MQ=255)

                                                           ttCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGa                                                                <  1:1281466/140‑1 (MQ=255)

                                                           ttCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGAAGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGa                                                                <  2:114602/140‑1 (MQ=255)

                                                                    tcGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCtcgt                                                       <  1:940245/140‑1 (MQ=255)

                                                                        gTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGtt                                                   >  2:686557/1‑140 (MQ=255)

                                                                               tgatgaAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTa                                            >  2:1342768/1‑140 (MQ=255)

                                                                                 atgaAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAAc                                          >  1:260355/1‑140 (MQ=255)

                                                                                  tgaAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGTACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAAcc                                         >  2:21543/1‑140 (MQ=255)

                                                                                   gaAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCg                                        <  1:1059487/140‑1 (MQ=255)

                                                                                      aCCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGttt                                     >  2:785247/1‑140 (MQ=255)

                                                                                      aCCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGttt                                     >  2:57812/1‑140 (MQ=255)

                                                                                      aCCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGttt                                     >  2:306204/1‑140 (MQ=255)

                                                                                         tGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGa                                  >  2:928879/1‑140 (MQ=255)

                                                                                             gCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAg                              <  2:978663/140‑1 (MQ=255)

                                                                                             gCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAg                              <  1:1342768/140‑1 (MQ=255)

                                                                                             gCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAg                              <  1:280572/140‑1 (MQ=255)

                                                                                             gCATCCTTCTTCTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAg                              <  1:446341/140‑1 (MQ=255)

                                                                                                   ttcttcTATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCgcgc                        <  1:928879/140‑1 (MQ=255)

                                                                                                    tcttctATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCgca                       <  1:431081/140‑1 (MQ=255)

                                                                                                       tctATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCgcagca                    <  2:288813/140‑1 (MQ=255)

                                                                                                        ctATTGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGAGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCAc                   <  1:1069883/140‑1 (MQ=255)

                                                                                                           ttGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACtgt                >  2:1291011/1‑140 (MQ=255)

                                                                                                           ttGTGGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACtgt                >  1:21426/1‑140 (MQ=255)

                                                                                                              tgGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCt             >  2:1229729/1‑140 (MQ=255)

                                                                                                              tgGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCt             >  1:207331/1‑140 (MQ=255)

                                                                                                              tgGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCt             >  2:673941/1‑140 (MQ=255)

                                                                                                              tgGATGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCt             >  2:485129/1‑140 (MQ=255)

                                                                                                                  tGCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCa         <  1:1291011/140‑1 (MQ=255)

                                                                                                                   gCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCac        >  1:114395/1‑140 (MQ=255)

                                                                                                                   gCTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCac        >  2:137139/1‑140 (MQ=255)

                                                                                                                    cTTTACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCacc       <  1:57812/140‑1 (MQ=255)

                                                                                                                     tttACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCacca      >  1:1215758/1‑140 (MQ=255)

                                                                                                                     tttACAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCacca      >  2:348215/1‑140 (MQ=255)

                                                                                                                        aCAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCacc       >  2:1414863/1‑136 (MQ=255)

                                                                                                                        aCAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCacc       <  1:1414863/136‑1 (MQ=255)

                                                                                                                         cAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCaccaacca  <  2:207331/140‑1 (MQ=255)

                                                                                                                         cAATGATT‑AAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCaccaacca  <  1:335644/140‑1 (MQ=255)

TCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCA  >  NC_000913/4163516‑4163777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A