Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,201,418	C→T	A91T (GCC→ACC)	zraP ←	zinc responsive, periplasmic protein with chaperone activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,201,418	0	C	T	99.0%	344.9 / ‑4.5	100	A91T (GCC→ACC)	zraP	zinc responsive, periplasmic protein with chaperone activity
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (49/50); total (50/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01

Only 100 of 101 total aligned reads displayed.

ATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTG  >  NC_000913/4201282‑4201552

atatGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGAc                                                                                                                                     >  2:371271/1‑140 (MQ=255)

atatGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGAc                                                                                                                                     <  1:1157234/140‑1 (MQ=255)

atatGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGAc                                                                                                                                     >  2:1157234/1‑140 (MQ=255)

atatGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGAc                                                                                                                                     >  2:1063220/1‑140 (MQ=255)

atatGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGAc                                                                                                                                     >  1:887002/1‑140 (MQ=255)

 tatGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGAcc                                                                                                                                    >  1:1039077/1‑140 (MQ=255)

 tatGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGAcc                                                                                                                                    >  1:739172/1‑140 (MQ=255)

 tatGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGAcc                                                                                                                                    >  1:494642/1‑140 (MQ=255)

 tatGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTATCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGAcc                                                                                                                                    >  2:1232295/1‑140 (MQ=255)

     aCCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGt                                                                                                                                >  1:892708/1‑140 (MQ=255)

     aCCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGt                                                                                                                                <  1:2208/140‑1 (MQ=255)

      ccGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGtt                                                                                                                               >  1:864483/1‑140 (MQ=255)

       cGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTa                                                                                                                              >  1:1244271/1‑140 (MQ=255)

        gCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTaa                                                                                                                             >  1:547495/1‑140 (MQ=255)

        gCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTaa                                                                                                                             >  1:227713/1‑140 (MQ=255)

        gCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTaa                                                                                                                             <  2:739172/140‑1 (MQ=255)

         ccaccaCCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAAt                                                                                                                            >  2:935548/1‑140 (MQ=255)

         ccaccaCCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAAt                                                                                                                            >  1:903686/1‑140 (MQ=255)

          caccacCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAAtt                                                                                                                           >  1:686686/1‑140 (MQ=255)

           accaccGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAAttt                                                                                                                          <  1:935548/140‑1 (MQ=255)

            ccaccGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAAtt                                                                                                                           <  1:271199/138‑1 (MQ=255)

            ccaccGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAAtt                                                                                                                           >  2:271199/1‑138 (MQ=255)

             caccGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTtg                                                                                                                        <  1:1372171/140‑1 (MQ=255)

              accGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTtgc                                                                                                                       <  2:641842/140‑1 (MQ=255)

                cGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTtgctg                                                                                                                     <  1:1280791/140‑1 (MQ=255)

                 gCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGCCGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTtgctgc                                                                                                                    <  2:1258888/140‑1 (MQ=255)

                 gCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTtgctgc                                                                                                                    >  1:27529/1‑140 (MQ=255)

                     ccgccgTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATc                                                                                                                >  2:667420/1‑140 (MQ=255)

                              cccatgcccatTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGtt                                                                                                       >  2:8275/1‑140 (MQ=255)

                                  tgcccatTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTcgcg                                                                                                   <  1:8275/140‑1 (MQ=255)

                                   gcccatTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTAt                                                                                                                 >  2:273889/1‑125 (MQ=255)

                                   gcccatTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTAt                                                                                                                 <  1:273889/125‑1 (MQ=255)

                                   gcccatTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTc                                                                                                      <  1:158213/136‑1 (MQ=255)

                                   gcccatTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTc                                                                                                      >  2:158213/1‑136 (MQ=255)

                                   gcccatTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCgg                                                                                                  >  1:492163/1‑140 (MQ=255)

                                        ttCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAAc                                                                                             >  2:1129027/1‑140 (MQ=255)

                                        ttCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAAc                                                                                             >  1:218558/1‑140 (MQ=255)

                                         tCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACa                                                                                            <  1:1274948/140‑1 (MQ=255)

                                           cGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAgg                                                                                          <  2:1227353/140‑1 (MQ=255)

                                           cGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAgg                                                                                          <  1:42254/140‑1 (MQ=255)

                                           cGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAgg                                                                                          <  1:371271/140‑1 (MQ=255)

                                           cGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAgg                                                                                          <  2:892708/140‑1 (MQ=255)

                                            ggCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAggg                                                                                         <  2:1364501/140‑1 (MQ=255)

                                              cTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCa                                                                                       >  1:1032088/1‑140 (MQ=255)

                                              cTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCa                                                                                       <  2:1039077/140‑1 (MQ=255)

                                              cTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCa                                                                                       <  2:494642/140‑1 (MQ=255)

                                               tCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCAt                                                                                      <  2:227713/140‑1 (MQ=255)

                                               tCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCAt                                                                                      <  2:547495/140‑1 (MQ=255)

                                               tCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCAt                                                                                      <  2:609432/140‑1 (MQ=255)

                                                ccGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCAtt                                                                                     <  1:1125413/140‑1 (MQ=255)

                                                ccGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCAtt                                                                                     <  1:70644/140‑1 (MQ=255)

                                                ccGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCAtt                                                                                     <  2:1277107/140‑1 (MQ=255)

                                                 cgcgcgGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTg                                                                                    >  2:1380842/1‑140 (MQ=255)

                                                  gcgcgGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGt                                                                                   <  1:1232295/140‑1 (MQ=255)

                                                   cgcgGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGc                                                                                        <  2:501397/134‑1 (MQ=255)

                                                   cgcgGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGc                                                                                        >  1:501397/1‑134 (MQ=255)

                                                   cgcgGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTa                                                                                  >  2:275162/1‑140 (MQ=255)

                                                   cgcgGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTa                                                                                  >  1:1184905/1‑140 (MQ=255)

                                                   cgcgGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTa                                                                                  >  1:514873/1‑140 (MQ=255)

                                                   cgcgGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTa                                                                                  >  1:349017/1‑140 (MQ=255)

                                                   cgcgGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAA‑TTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCa                                                                                       >  1:1278670/1‑134 (MQ=255)

                                                   cgcgGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAA‑TTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCa                                                                                       <  2:1278670/134‑1 (MQ=255)

                                                        aaTACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCAt                                                                             <  2:1184905/140‑1 (MQ=255)

                                                           aCCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAAc                                                                          <  1:275162/140‑1 (MQ=255)

                                                                  tCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGt                                                                   >  1:468247/1‑140 (MQ=255)

                                                                   cAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTc                                                                  <  2:1312612/140‑1 (MQ=255)

                                                                    aGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCa                                                                 >  1:1190974/1‑140 (MQ=255)

                                                                    aGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCa                                                                 >  2:7857/1‑140 (MQ=255)

                                                                         tCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGt                                                            >  2:349392/1‑140 (MQ=255)

                                                                              atatCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTg                                                       <  2:1032088/140‑1 (MQ=255)

                                                                                 tctcGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTg                                                    <  2:1187327/140‑1 (MQ=255)

                                                                                  ctcGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGc                                                   <  1:667420/140‑1 (MQ=255)

                                                                                     gTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTACATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGt                                                >  2:431880/1‑140 (MQ=255)

                                                                                        tCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTgcg                                             <  2:1142948/140‑1 (MQ=255)

                                                                                         cACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTgcgc                                            >  2:1334807/1‑140 (MQ=255)

                                                                                           cccGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCa                                                  >  1:692173/1‑132 (MQ=255)

                                                                                           cccGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCa                                                  <  2:692173/132‑1 (MQ=255)

                                                                                           cccGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCgctg                                          >  2:345502/1‑140 (MQ=255)

                                                                                           cccGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCgctg                                          >  1:569982/1‑140 (MQ=255)

                                                                                                      tCTAACGACTGACGCAAATTCTACATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTa                               <  1:431880/140‑1 (MQ=255)

                                                                                                           cGACTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGt                          <  1:345502/140‑1 (MQ=255)

                                                                                                             aCTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCa                        >  1:606962/1‑140 (MQ=255)

                                                                                                             aCTGACGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCa                        <  2:686686/140‑1 (MQ=255)

                                                                                                                 aCGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTAt                    >  1:669720/1‑140 (MQ=255)

                                                                                                                 aCGCAAATTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTAt                    >  2:947799/1‑140 (MQ=255)

                                                                                                                       aTTCTCCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGAttt              >  2:1192503/1‑140 (MQ=255)

                                                                                                                          ctcCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCt           <  1:793518/140‑1 (MQ=255)

                                                                                                                           tcCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCt           <  1:844957/139‑1 (MQ=255)

                                                                                                                           tcCATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCt           >  2:844957/1‑139 (MQ=255)

                                                                                                                            ccATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCTGc         <  2:864483/140‑1 (MQ=255)

                                                                                                                             cATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCTGcc        <  2:185222/140‑1 (MQ=255)

                                                                                                                             cATCTCTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATGATGGATTTTCTGcc        <  1:349392/140‑1 (MQ=255)

                                                                                                                               tctcTTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCTGCCAc      <  1:1129027/140‑1 (MQ=255)

                                                                                                                                ctctTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACg     <  2:492163/140‑1 (MQ=255)

                                                                                                                                ctctTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACg     <  2:606962/140‑1 (MQ=255)

                                                                                                                                ctctTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACg     <  2:349017/140‑1 (MQ=255)

                                                                                                                                ctctTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACg     <  1:803405/140‑1 (MQ=255)

                                                                                                                                 tctTTGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGc    <  1:1380842/140‑1 (MQ=255)

                                                                                                                                 tctTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGc    >  1:1379008/1‑140 (MQ=255)

                                                                                                                                   tttGGTGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGctg  >  1:127678/1‑140 (MQ=255)

ATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTTTGAGCGTAAAAGTCATTATGGATTTTCTGCCACGCTG  >  NC_000913/4201282‑4201552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A