Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,422,800	T→C	R205R (CGT→CGC)	ulaD →	3‑keto‑L‑gulonate‑6‑phosphate decarboxylase UlaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,422,800	0	T	C	100.0%	206.1 / NA	57	R205R (CGT→CGC)	ulaD	3‑keto‑L‑gulonate‑6‑phosphate decarboxylase UlaD
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (31/26); total (31/26)

GGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGT  >  NC_000913/4422672‑4422936

ggCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACg                                                                                                                               >  1:420034/1‑140 (MQ=255)

 gCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGt                                                                                                                              >  2:1293594/1‑140 (MQ=255)

   ttCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTc                                                                                                                            <  1:275735/140‑1 (MQ=255)

     cAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCa                                                                                                                          <  1:116045/140‑1 (MQ=255)

          tcaccgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCt                                                                                                                     <  1:426752/140‑1 (MQ=255)

           caccgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTg                                                                                                                    >  1:1190799/1‑140 (MQ=255)

               gtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACt                                                                                                                >  2:716340/1‑140 (MQ=255)

                tcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                               <  1:716340/140‑1 (MQ=255)

                tcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                               <  1:1367893/140‑1 (MQ=255)

                tcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                               <  2:157678/140‑1 (MQ=255)

                  accgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACtgtg                                                                                                             <  1:1170916/140‑1 (MQ=255)

                    cgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTggg                                                                                                           <  1:1248889/140‑1 (MQ=255)

                        ggCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTa                                                                                                       >  2:1314104/1‑140 (MQ=255)

                          ccTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAg                                                                                                     <  1:1314104/140‑1 (MQ=255)

                           cTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                                                    <  2:522371/140‑1 (MQ=255)

                           cTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                                                    >  2:1129380/1‑140 (MQ=255)

                             ggCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGAGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACATTCCATCGCTGAACTGTGGGGCTAAGGAg                                                                                                  <  2:1036260/140‑1 (MQ=255)

                              gcgcTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGc                                                                                                 >  1:233615/1‑140 (MQ=255)

                                   ggAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCt                                                                                                        >  2:689471/1‑128 (MQ=255)

                                   ggAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCt                                                                                                        <  1:689471/128‑1 (MQ=255)

                                   ggAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGata                                                                                            >  2:999185/1‑140 (MQ=255)

                                    gAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGatat                                                                                           >  1:677242/1‑140 (MQ=255)

                                     aaGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATAtg                                                                                          <  2:1190799/140‑1 (MQ=255)

                                         tCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATAtgttgt                                                                                      <  1:385148/140‑1 (MQ=255)

                                                   ttCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAAt                                                                            >  2:402339/1‑140 (MQ=255)

                                                   ttCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAAt                                                                            >  2:1072634/1‑140 (MQ=255)

                                                             ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                                  >  1:531111/1‑140 (MQ=255)

                                                             ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                                  >  1:804560/1‑140 (MQ=255)

                                                             ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                                  <  1:999185/140‑1 (MQ=255)

                                                             ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                                  >  1:1394651/1‑140 (MQ=255)

                                                             ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                                  <  1:1293594/140‑1 (MQ=255)

                                                             ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                                  <  2:777250/140‑1 (MQ=255)

                                                               ccGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCAt                                                                <  2:238120/140‑1 (MQ=255)

                                                                  aTTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTa                                                             >  2:619491/1‑140 (MQ=255)

                                                                  aTTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTa                                                             >  1:999397/1‑140 (MQ=255)

                                                                       cGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCAt                                                                <  2:887220/132‑1 (MQ=255)

                                                                       cGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCAt                                                                >  1:887220/1‑132 (MQ=255)

                                                                        gTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGaaaa                                                       >  2:1292704/1‑140 (MQ=255)

                                                                              aTCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGGGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCt                                                 <  2:677242/140‑1 (MQ=255)

                                                                                cgcgGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTcc                                               <  2:531111/140‑1 (MQ=255)

                                                                                  cgGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTcccc                                             >  2:911877/1‑140 (MQ=255)

                                                                                  cgGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACCGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTcccc                                             >  1:172034/1‑140 (MQ=255)

                                                                                    ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATg                                                           <  2:1267293/124‑1 (MQ=255)

                                                                                    ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATg                                                           >  1:1267293/1‑124 (MQ=255)

                                                                                    ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAACCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCccgc                                           >  2:1406585/1‑140 (MQ=255)

                                                                                              tCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgc                                 <  1:940554/140‑1 (MQ=255)

                                                                                                 gTGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctgg                              >  2:119047/1‑140 (MQ=255)

                                                                                                  tGATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggc                             >  2:722506/1‑140 (MQ=255)

                                                                                                   gATGCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggct                            >  1:568070/1‑140 (MQ=255)

                                                                                                      gCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGa                         <  1:1129380/140‑1 (MQ=255)

                                                                                                      gCCGCTTCTCCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGa                         <  1:1072634/140‑1 (MQ=255)

                                                                                                              tcCGGTGGAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGc                 >  2:1160070/1‑140 (MQ=255)

                                                                                                                     gAAGCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGc          >  2:864183/1‑140 (MQ=255)

                                                                                                                        gCCGCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCaaa       >  2:293094/1‑140 (MQ=255)

                                                                                                                           gCACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAAc    >  2:511301/1‑140 (MQ=255)

                                                                                                                            cACGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACg   >  1:617915/1‑140 (MQ=255)

                                                                                                                             aCGCCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGt  <  1:511301/140‑1 (MQ=255)

GGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGT  >  NC_000913/4422672‑4422936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A