Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	191	83.5	2830284	99.3%	2810472	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,515,272	T→C	Y469C (TAC→TGC)	fecA ←	ferric citrate outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,515,272	0	T	C	100.0%	316.8 / NA	87	Y469C (TAC→TGC)	fecA	ferric citrate outer membrane transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (40/47); total (40/47)

TGTGATGGCGTTGTTCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGT  >  NC_000913/4515139‑4515407

tgtgATGGCGTTGTTCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGTAACGCGTATCGCGGTCGCAAGGGc                                                                                                                                   <  2:713389/140‑1 (MQ=255)

  tgATGGCGTTGTTCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCtt                                                                                                                                 >  1:1223568/1‑140 (MQ=255)

      ggCGTTGTTCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGc                                                                                                                             <  2:107651/140‑1 (MQ=255)

       gCGTTGTTCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGcc                                                                                                                            >  1:690634/1‑140 (MQ=255)

        cGTTGTTCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCg                                                                                                                           >  2:608558/1‑140 (MQ=255)

        cGTTGTTCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCg                                                                                                                           <  1:591012/140‑1 (MQ=255)

         gttgttCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCgg                                                                                                                          >  2:806738/1‑140 (MQ=255)

            gttCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACg                                                                                                                       >  1:1276294/1‑140 (MQ=255)

            gttCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACg                                                                                                                       >  2:1078323/1‑140 (MQ=255)

            gttCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACg                                                                                                                       >  1:623740/1‑140 (MQ=255)

              tCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGc                                                                                                                     >  1:51165/1‑140 (MQ=255)

                  gTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACt                                                                                                                 >  2:504760/1‑140 (MQ=255)

                       aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTg                                                                                                                <  1:317095/136‑1 (MQ=255)

                       aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTg                                                                                                                >  2:317095/1‑136 (MQ=255)

                       aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCg                                                                                                            >  2:931270/1‑140 (MQ=255)

                       aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCg                                                                                                            >  1:375460/1‑140 (MQ=255)

                       aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCg                                                                                                            >  2:1308239/1‑140 (MQ=255)

                       aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCg                                                                                                            >  2:1185667/1‑140 (MQ=255)

                       aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCg                                                                                                            >  1:673502/1‑140 (MQ=255)

                       aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCg                                                                                                            >  1:986616/1‑140 (MQ=255)

                       aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCg                                                                                                            >  2:886482/1‑140 (MQ=255)

                       aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCg                                                                                                            >  1:107576/1‑140 (MQ=255)

                       aCTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCg                                                                                                            >  2:842743/1‑140 (MQ=255)

                           gATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCgctgc                                                                                                        <  1:806738/140‑1 (MQ=255)

                            atatGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCgctgct                                                                                                       >  1:792328/1‑140 (MQ=255)

                               tGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggt                                                                                                    <  1:1272731/140‑1 (MQ=255)

                                 ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                  <  2:1276294/140‑1 (MQ=255)

                                 ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                  >  1:68274/1‑140 (MQ=255)

                                 ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                  <  1:608558/140‑1 (MQ=255)

                                 ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                  >  2:8162/1‑140 (MQ=255)

                                 ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                  <  2:1021595/140‑1 (MQ=255)

                                 ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                  <  1:1184097/140‑1 (MQ=255)

                                 ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                  <  1:1221230/140‑1 (MQ=255)

                                 ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                  <  2:1106662/140‑1 (MQ=255)

                                 ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                  <  1:456771/140‑1 (MQ=255)

                                 ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                  <  2:1223568/140‑1 (MQ=255)

                                 ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                  <  1:1308239/140‑1 (MQ=255)

                                 ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                  <  2:255072/140‑1 (MQ=255)

                                 ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                  <  2:138045/140‑1 (MQ=255)

                                 ttCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggtga                                                                                                  <  2:127395/140‑2 (MQ=255)

                                    gAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCgg                                                                                               <  1:1229928/140‑1 (MQ=255)

                                    gAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCgg                                                                                               <  1:706111/140‑1 (MQ=255)

                                      aaCGCTTTCCCGGCGTGTTTGTTCAGTTGCCGATGTCGATTTTGCCATCCAGAGACCAGGCGTGCGTCTCGGTGCCGGAACGCGTATCGGGGGCGCAAGGGCTTGATCCGGACGGCAACTGCCCGATGCTGGTGGCGgtg                                                                                             <  2:998574/140‑1 (MQ=255)

                                      aaCGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGgtg                                                                                             >  1:101139/1‑140 (MQ=255)

                                      aaCGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGgtg                                                                                             <  2:364545/140‑1 (MQ=255)

                                        cGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTgta                                                                                           <  2:51165/140‑1 (MQ=255)

                                        cGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTgta                                                                                           <  2:150341/140‑1 (MQ=255)

                                                   cGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                  >  1:1355142/1‑122 (MQ=255)

                                                   cGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCtggtgg                                                                                                  <  2:1355142/122‑1 (MQ=255)

                                                   cGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCAttt                                                                                >  2:1275501/1‑140 (MQ=255)

                                                    gTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTc                                                                               <  1:1078323/140‑1 (MQ=255)

                                                     tGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCa                                                                              <  2:986616/140‑1 (MQ=255)

                                                         ggTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTc                                                                               <  1:1023866/135‑1 (MQ=255)

                                                         ggTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTc                                                                               >  2:1023866/1‑135 (MQ=255)

                                                             cAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGa                                                                      <  1:504760/140‑1 (MQ=255)

                                                             cAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGa                                                                      <  1:1145375/140‑1 (MQ=255)

                                                             cAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGa                                                                      >  1:1203524/1‑140 (MQ=255)

                                                               gTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGatt                                                                    <  1:430582/140‑1 (MQ=255)

                                                                       tGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCaaa                                                            <  1:1226914/140‑1 (MQ=255)

                                                                       tGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCaaa                                                            <  1:575608/140‑1 (MQ=255)

                                                                             ttttGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGa                                                      <  2:445707/140‑1 (MQ=255)

                                                                                  tCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcc                                                 <  1:8162/140‑1 (MQ=255)

                                                                                  tCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcc                                                 <  1:1275501/140‑1 (MQ=255)

                                                                                  tCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgcc                                                 <  1:512992/140‑1 (MQ=255)

                                                                                    aTCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAgccca                                               <  2:68274/140‑1 (MQ=255)

                                                                                                ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGt                                   >  1:178290/1‑140 (MQ=255)

                                                                                                ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGt                                   >  2:1231750/1‑140 (MQ=255)

                                                                                                ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGt                                   >  1:558076/1‑140 (MQ=255)

                                                                                                ggCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGt                                   >  2:569850/1‑140 (MQ=255)

                                                                                                    tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTgcgc                               >  2:979230/1‑140 (MQ=255)

                                                                                                    tGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTgcgc                               >  1:1178944/1‑140 (MQ=255)

                                                                                                      cgcCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTc                                     >  2:1317280/1‑132 (MQ=255)

                                                                                                      cgcCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTc                                     <  1:1317280/132‑1 (MQ=255)

                                                                                                      cgcCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCgg                             >  1:1180942/1‑140 (MQ=255)

                                                                                                       gcCTCGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTTATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGa                            >  1:1037606/1‑140 (MQ=255)

                                                                                                           cGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAggg                        >  1:1334973/1‑140 (MQ=255)

                                                                                                           cGGTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAggg                        <  2:1176554/140‑1 (MQ=255)

                                                                                                            ggTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGc                       <  1:865949/140‑1 (MQ=255)

                                                                                                            ggTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGc                       <  2:1334973/140‑1 (MQ=255)

                                                                                                             gTGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGcc                      <  2:1178944/140‑1 (MQ=255)

                                                                                                              tGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCg                     <  2:1037606/140‑1 (MQ=255)

                                                                                                              tGCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCg                     <  1:142027/140‑1 (MQ=255)

                                                                                                               gCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGa                    <  2:558076/140‑1 (MQ=255)

                                                                                                               gCCGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGa                    <  2:1203524/140‑1 (MQ=255)

                                                                                                                 cGGAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATc                  <  1:477896/140‑1 (MQ=255)

                                                                                                                   gAACGCGTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCAt                >  2:848190/1‑140 (MQ=255)

                                                                                                                        cgTATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGa           <  2:673502/140‑1 (MQ=255)

                                                                                                                          tATCGCGGTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGGAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATc         >  2:977008/1‑140 (MQ=255)

                                                                                                                                 gTCGCAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGt  >  2:35205/1‑140 (MQ=255)

TGTGATGGCGTTGTTCTGGTATGACTCGATATGTTCGAAACGCATACCCGGCGTGATGGTCCAGTTGCCGATGTCGATTTTGTCATCCAGATACCAGGCGTGCGCCTCGGTGCCGGAACGCGTATCGCGGTCGTAAGGGCTTGAGCCGGACGGCAACTGCCCGCTGCTGGTGGCGGTGTAGTAACGCATTTCATGCGTTGATTCATTCAAATAGCGATAGCCCACGCCCACTTCGTGCGCGGAAGGGCCGATCATAAAGATCTGGCTGT  >  NC_000913/4515139‑4515407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A