Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	54,212	A→G	V164A (GTG→GCG)	surA ←	peptidyl‑prolyl cis‑trans isomerase SurA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	54,212	0	A	G	96.2%	171.4 / ‑2.3	53	V164A (GTG→GCG)	surA	peptidyl‑prolyl cis‑trans isomerase SurA
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (30/21); total (31/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

CGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTC  >  NC_000913/54078‑54338

cGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCctgc                                                                                                                           <  2:398915/140‑1 (MQ=255)

  ttACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCctgctg                                                                                                                         >  2:457568/1‑140 (MQ=255)

          cTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAggg                                                                                                                 >  2:61178/1‑140 (MQ=255)

            gATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGAt                                                                                                               >  1:300459/1‑140 (MQ=255)

             aTCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGAtt                                                                                                              <  1:194650/140‑1 (MQ=255)

                    aTGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTc                                                                                                       >  1:47504/1‑140 (MQ=255)

                     tGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTcc                                                                                                      <  2:300459/140‑1 (MQ=255)

                        cgcgcgcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCGGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGc                                                                                                   >  1:50917/1‑140 (MQ=255)

                        cgcgcgcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGc                                                                                                   >  2:232947/1‑140 (MQ=255)

                        cgcgcgcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGc                                                                                                   >  1:343642/1‑140 (MQ=255)

                          cgcgcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCgg                                                                                                 >  1:59318/1‑140 (MQ=255)

                            cgcCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCa                                                                                               >  1:110695/1‑140 (MQ=255)

                                               ttCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCGGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGacgacg                                                                            <  2:481496/140‑1 (MQ=255)

                                                tCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGc                                                                           <  2:55777/140‑1 (MQ=255)

                                                 cACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCa                                                                          <  1:454107/140‑1 (MQ=255)

                                                   ccTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCAcc                                                                        <  2:110695/140‑1 (MQ=255)

                                                              gTCGGGTTTTCCGGCAGCGGGATCGGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACg                                                             <  2:50917/140‑1 (MQ=255)

                                                              gTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACg                                                             <  2:552098/140‑1 (MQ=255)

                                                              gTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACg                                                             >  2:222038/1‑140 (MQ=255)

                                                              gTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACg                                                             >  1:737398/1‑140 (MQ=255)

                                                              gTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCCTCGTTTTGGTTCCCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACg                                                             <  2:682102/140‑1 (MQ=255)

                                                                 gggTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACg                                                             <  1:142689/137‑1 (MQ=255)

                                                                 gggTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACg                                                             >  2:142689/1‑137 (MQ=255)

                                                                 gggTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCAc                                                          >  1:615013/1‑140 (MQ=255)

                                                                            cAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATaa                                               >  1:652416/1‑140 (MQ=255)

                                                                                 ggATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCagag                                                   >  1:204334/1‑131 (MQ=255)

                                                                                 ggATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCagag                                                   <  2:204334/131‑1 (MQ=255)

                                                                                     cAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCtt                                      <  2:615013/140‑1 (MQ=255)

                                                                                       ggATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTg                                    <  1:457568/140‑1 (MQ=255)

                                                                                         aTGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCg                                  <  1:61178/140‑1 (MQ=255)

                                                                                            tgGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGAt                               <  2:59318/140‑1 (MQ=255)

                                                                                             ggCTCGGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATc                              >  1:39013/1‑140 (MQ=255)

                                                                                              gCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGAt                               <  2:870971/138‑1 (MQ=255)

                                                                                              gCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGAt                               >  1:870971/1‑138 (MQ=255)

                                                                                              gCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGGTACGCACTTCAGAGATAATCATCTCTTTGCGGATCt                             >  2:608156/1‑140 (MQ=255)

                                                                                                  aGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCGGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGtt                         >  1:4957/1‑140 (MQ=255)

                                                                                                  aGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGtt                         >  2:134085/1‑140 (MQ=255)

                                                                                                   ggTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTa                        >  2:71516/1‑140 (MQ=255)

                                                                                                       cAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGAt                    <  2:445666/140‑1 (MQ=255)

                                                                                                       cAGCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGAt                    <  1:222038/140‑1 (MQ=255)

                                                                                                         gCTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAg                  >  1:795667/1‑140 (MQ=255)

                                                                                                          cTCAGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAgg                 <  1:179520/140‑1 (MQ=255)

                                                                                                             aGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAgg                 <  1:68018/137‑1 (MQ=255)

                                                                                                             aGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAgg                 >  2:68018/1‑137 (MQ=255)

                                                                                                             aGTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGgtgt              >  1:167797/1‑140 (MQ=255)

                                                                                                              gTGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGtgtt             >  1:755619/1‑140 (MQ=255)

                                                                                                               tGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGt                          >  2:815753/1‑126 (MQ=255)

                                                                                                               tGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGt                          <  1:815753/126‑1 (MQ=255)

                                                                                                               tGCTGGCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGtgttg            <  2:39013/140‑1 (MQ=255)

                                                                                                                    gCGTCGTTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGtt       >  1:809690/1‑140 (MQ=255)

                                                                                                                       tcgtTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAg    >  1:779145/1‑140 (MQ=255)

                                                                                                                         gtTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTc  >  2:54723/1‑140 (MQ=255)

                                                                                                                         gtTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTc  >  2:388025/1‑140 (MQ=255)

                                                                                                                         gtTTTGGTTACCCGCCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGCGTTGTAGTTCAGTc  >  2:785953/1‑140 (MQ=255)

CGTTACGCGCCTGATCGACAATGGCGCGCGCCTGGCTTTCCGCTTCGTTCACCTGATCAGAGGTCGGGTTTTCCGGCAGCGGGATCAGGATGTGGCTCAGGTTCAGCTCAGTGCTGGCGTCGTTTTGGTTACCCACCTGCTGCGCCAGGGATTCGACTTCCTGCGGCAGGATGGTGATGCGACGACGCACCTCGTTGTTACGCACTTCAGAGATAATCATCTCTTTGCGGATCTGGTTACGATAGGTGTTGTAGTTCAGTC  >  NC_000913/54078‑54338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A