Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R1 163 52.9 1786516 99.3% 1774010 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,515,631 (A)5→4 coding (54/1425 nt) patD → gamma‑aminobutyraldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,515,6270A.100.0% 166.9 / NA 40coding (50/1425 nt)patDgamma‑aminobutyraldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (23/17);  total (23/17)

ATGTTGGTAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAACCACGCCGAA  >  NC_000913/1515488‑1515759
                                                                                                                                           |                                                                                                                                    
aTGTTGGTAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGTGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGa                                                                                                                                      >  1:157655/1‑140 (MQ=255)
  gTTGGTAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGaaa                                                                                                                                    >  2:532246/1‑140 (MQ=255)
       tAACAACCTTTCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGaaaacagc                                                                                                                               <  2:723769/140‑5 (MQ=255)
       tAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAATGGGaaaacagc                                                                                                                               >  1:410920/1‑136 (MQ=255)
       tAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGaaaacagc                                                                                                                               <  2:761625/140‑5 (MQ=255)
       tAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGaaaacagc                                                                                                                               <  1:271687/140‑5 (MQ=255)
       tAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGaaaacagc                                                                                                                               >  1:282874/1‑136 (MQ=255)
       tAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGaaaacagc                                                                                                                               <  2:747152/140‑5 (MQ=255)
       tAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGaaaacagc                                                                                                                               <  2:357738/140‑5 (MQ=255)
        aacaacCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGcc                                                                                                                             <  2:302513/140‑1 (MQ=255)
        aacaacCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGcc                                                                                                                             <  1:785452/140‑1 (MQ=255)
         acaacCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCt                                                                                                                            <  2:96837/140‑1 (MQ=255)
           aacCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAATCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGt                                                                                                                          <  2:888890/140‑1 (MQ=255)
                  ccGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGaaaacagc                                                                                                                               >  1:197104/1‑125 (MQ=255)
                  ccGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGaaaacagc                                                                                                                               <  2:197104/129‑5 (MQ=255)
                       ccTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGc                                                                                                              >  2:624266/1‑140 (MQ=255)
                                cTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAATGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGa                                                                                                     <  2:410920/140‑1 (MQ=255)
                                cTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGa                                                                                                     >  2:219485/1‑140 (MQ=255)
                                cTGGCGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGa                                                                                                     >  2:401348/1‑140 (MQ=255)
                                 tggtggCTAACCCGCGAAGGCAACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGAc                                                                                                    >  2:285068/1‑140 (MQ=255)
                                            ccGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGa                                                                                         <  1:624266/140‑1 (MQ=255)
                                                   ggCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGcc                                                                                  <  2:364658/140‑1 (MQ=255)
                                                             gTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCg                                                                        <  2:282874/140‑1 (MQ=255)
                                                              tCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGc                                                                       >  1:644114/1‑140 (MQ=255)
                                                                   aaCCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGc                                                                  <  1:702112/140‑1 (MQ=255)
                                                                     ccACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAg                                                                <  2:644114/140‑1 (MQ=255)
                                                                       aCTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGt                                                              <  1:285068/140‑1 (MQ=255)
                                                                        cTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTc                                                             >  2:39177/1‑140 (MQ=255)
                                                                            tACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGAtg                                                         >  2:721204/1‑140 (MQ=255)
                                                                                ggAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGAtgctgc                                                     >  1:603992/1‑140 (MQ=255)
                                                                                ggAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGAtgctgc                                                     <  2:603992/140‑1 (MQ=255)
                                                                                  aaTATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGctgctg                                                   >  1:465357/1‑140 (MQ=255)
                                                                                     atgctatgcAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTgc                                                >  2:160684/1‑140 (MQ=255)
                                                                                         tatgcAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTgcgcgc                                            >  2:170006/1‑140 (MQ=255)
                                                                                           tgcAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCgg                                          <  1:721204/140‑1 (MQ=255)
                                                                                           tgcAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCgg                                          <  2:498633/140‑1 (MQ=255)
                                                                                           tgcAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCgg                                          >  2:303385/1‑140 (MQ=255)
                                                                                           tgcAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCgg                                          >  1:604018/1‑140 (MQ=255)
                                                                                            gcAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGc                                         <  2:643719/140‑1 (MQ=255)
                                                                                                cATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGAt                                     >  2:44614/1‑140 (MQ=255)
                                                                                                                aCGGAGAACTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAAt                     <  1:39177/140‑1 (MQ=255)
                                                                                                                      aaCTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGc               >  2:511295/1‑140 (MQ=255)
                                                                                                                      aaCTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGc               >  2:552818/1‑140 (MQ=255)
                                                                                                                      aaCTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGc               >  1:41360/1‑140 (MQ=255)
                                                                                                                      aaCTGGTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGc               >  2:1735/1‑140 (MQ=255)
                                                                                                                          ggTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAAc           <  1:160684/140‑1 (MQ=255)
                                                                                                                           gTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAAcc          >  2:438209/1‑140 (MQ=255)
                                                                                                                           gTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAAcc          >  2:427208/1‑140 (MQ=255)
                                                                                                                           gTTAGCGGCGAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAAcc          >  2:379175/1‑140 (MQ=255)
                                                                                                                                cggcgAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAACCACGcc     >  2:609167/1‑140 (MQ=255)
                                                                                                                                   cgAAGGGG‑AAAACAGCCTGTCTATAATCCGGCAACGGGAGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAACCACGCCGaa  >  2:288341/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                    
ATGTTGGTAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCGACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACATAAGTTACTGATTAACGGAGAACTGGTTAGCGGCGAAGGGGAAAAACAGCCTGTCTATAATCCGGCAACGGGGGACGTTTTACTGGAAATTGCCGAGGCATCCGCAGAGCAGGTCGATGCTGCTGTGCGCGCGGCAGATGCAGCATTTGCCGAATGGGGGCAAACCACGCCGAA  >  NC_000913/1515488‑1515759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A