Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,874,137	(A)_6→7	coding (46/105 nt)	yoaI ←	protein YoaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,874,131	1	.	A	100.0%	167.4 / NA	47	A18S (GCC→TCC)	yoaI	protein YoaI
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (22/25); total (22/25)

TGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGC‑AAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGAT  >  NC_000913/1873992‑1874260

tGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGc                                                                                                                                    >  2:9021/1‑140 (MQ=255)

  cTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGC‑aa                                                                                                                                 >  1:144375/1‑140 (MQ=255)

           tCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGC‑aaaaaaacgat                                                                                                                        >  2:463556/1‑135 (MQ=255)

            ccGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATg                                                                                                                        >  2:887026/1‑140 (MQ=255)

            ccGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATg                                                                                                                        <  2:729558/140‑1 (MQ=255)

                   tttGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGAt                                                                                                                 <  2:798761/140‑1 (MQ=255)

                            cgcgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATg                                                                                                                        <  2:813031/124‑1 (MQ=255)

                            cgcgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATg                                                                                                                        >  1:813031/1‑124 (MQ=255)

                            cgcgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAgtgt                                                                                                        >  1:28415/1‑140 (MQ=255)

                            cgcgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAgtgt                                                                                                        >  2:677461/1‑140 (MQ=255)

                                  ttattTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGAc                                                                                                  <  1:434847/140‑1 (MQ=255)

                                  ttattTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGAc                                                                                                  <  2:28415/140‑1 (MQ=255)

                                  ttattTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGAc                                                                                                  <  2:175537/140‑1 (MQ=255)

                                         cgcattgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACAtt                                                                                           <  1:887026/140‑1 (MQ=255)

                                           cattgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTg                                                                                         >  2:16832/1‑140 (MQ=255)

                                            attgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGa                                                                                        <  2:156555/140‑1 (MQ=255)

                                             ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAATAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGAt                                                                                       <  2:710408/140‑1 (MQ=255)

                                             ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGAt                                                                                       <  1:853383/140‑1 (MQ=255)

                                             ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGAt                                                                                       >  1:21077/1‑140 (MQ=255)

                                             ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGAt                                                                                       >  2:676246/1‑140 (MQ=255)

                                               gcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCg                                                                                     >  1:366352/1‑140 (MQ=255)

                                                 gcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGtt                                                                                   <  1:162271/140‑1 (MQ=255)

                                                         aaCTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCAt                                                                           <  1:677461/140‑1 (MQ=255)

                                                           cTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGc                                                                         <  2:144375/140‑1 (MQ=255)

                                                              gTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGcccc                                                                      >  2:566675/1‑140 (MQ=255)

                                                               tCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGccccc                                                                     >  2:768028/1‑140 (MQ=255)

                                                               tCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGccccc                                                                     >  2:249447/1‑140 (MQ=255)

                                                               tCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGccccc                                                                     >  1:23431/1‑140 (MQ=255)

                                                                 aaTTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCgg                                                                   >  1:291409/1‑140 (MQ=255)

                                                                                gCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTc                                                    <  2:746225/140‑1 (MQ=255)

                                                                                gCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTc                                                    <  2:212535/140‑1 (MQ=255)

                                                                                    aaCTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGt                                                <  1:90635/140‑1 (MQ=255)

                                                                                    aaCTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGt                                                <  2:366352/140‑1 (MQ=255)

                                                                                        aGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG‑CCCCGGGAGATAGCTGTTTTCACGTTATTg                                           <  2:21077/140‑1 (MQ=255)

                                                                                           cGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTc                                                    >  1:852921/1‑129 (MQ=255)

                                                                                           cGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTc                                                    <  2:852921/129‑1 (MQ=255)

                                                                                           cGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGtt                                         >  2:614219/1‑140 (MQ=255)

                                                                                           cGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGtt                                         >  2:737022/1‑140 (MQ=255)

                                                                                           cGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGtt                                         >  1:594461/1‑140 (MQ=255)

                                                                                           cGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGtt                                         >  2:154141/1‑140 (MQ=255)

                                                                                              tCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGc                                      >  2:558340/1‑140 (MQ=255)

                                                                                                cGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGt                                    <  1:566675/140‑1 (MQ=255)

                                                                                                   tCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGca                                 <  1:9021/140‑1 (MQ=255)

                                                                                                   tCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGca                                 >  1:759507/1‑140 (MQ=255)

                                                                                                           aagaaCGGACAAAACCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGc                         <  1:558340/140‑1 (MQ=255)

                                                                                                                     aaaaCCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTtgatga               <  1:249447/140‑1 (MQ=255)

                                                                                                                     aaaaCCAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTtgatga               <  1:768028/140‑1 (MQ=255)

                                                                                                                         ccAGTACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGt           <  2:291409/140‑1 (MQ=255)

                                                                                                                             tACAACAGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGc       >  2:771039/1‑140 (MQ=255)

                                                                                                                                  caGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGAt  >  2:451908/1‑140 (MQ=255)

                                                                                                                                  caGCAATGGCAAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGAt  >  1:875442/1‑140 (MQ=255)

TGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGC‑AAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGAT  >  NC_000913/1873992‑1874260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A