Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,123,249	A→G	D424D (GAT→GAC)	cpsB ←	mannose‑1‑phosphate guanylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,123,249	0	A	G	100.0%	143.5 / NA	41	D424D (GAT→GAC)	cpsB	mannose‑1‑phosphate guanylyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/17); total (24/17)

CACATCATCCTCTTCGAGATAAGAGCCGGAGCGCACTTCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATATCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCTGGTAGCGGTCGCCCGCGTCGAT  >  NC_000913/2123117‑2123386

cacaTCATCCTCTTCGAGATAAGAGCCGGAGCGCACTTCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCAt                                                                                                                                    <  1:482399/140‑1 (MQ=255)

     catcCTCTTCGAGATAAGAGCCGGAGCGCACTTCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATg                                                                                                                               >  2:823886/1‑140 (MQ=255)

       tcCTCTTCGAGATAAGAGCCGGAGCGCACTTCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGt                                                                                                                             >  2:344791/1‑140 (MQ=255)

          tctTCGAGATAAGAGCCGGAGCGCACTTCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCGCCACCAATGGTGAc                                                                                                                          >  2:549497/1‑140 (MQ=255)

                      gagCCGGAGCGCACTTCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTcc                                                                                                              >  1:683151/1‑140 (MQ=255)

                         ccGGAGCGCACTTCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCcgc                                                                                                           <  2:503157/140‑1 (MQ=255)

                           ggAGCGCACTTCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGa                                                                                                         >  2:36714/1‑140 (MQ=255)

                           ggAGCGCACTTCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGa                                                                                                         <  2:106965/140‑1 (MQ=255)

                            gAGCGCACTTCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGAc                                                                                                        <  1:890191/140‑1 (MQ=255)

                            gAGCGCACTTCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGAc                                                                                                        >  1:539459/1‑140 (MQ=255)

                                gcACTTCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAacc                                                                                                    <  2:539459/140‑1 (MQ=255)

                                 cACTTCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAacca                                                                                                   >  1:742743/1‑140 (MQ=255)

                                         ttAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAgtgt                                                                                           <  1:823886/140‑1 (MQ=255)

                                            aaTCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAacc                                                                                                    <  1:321241/128‑1 (MQ=255)

                                            aaTCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAacc                                                                                                    >  2:321241/1‑128 (MQ=255)

                                              tCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCcgc                                                                                      >  1:860797/1‑140 (MQ=255)

                                              tCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCcg                                                                                       <  1:73868/139‑1 (MQ=255)

                                              tCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCcg                                                                                       >  2:73868/1‑139 (MQ=255)

                                                 aGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCcgcgcg                                                                                   >  2:681336/1‑140 (MQ=255)

                                                 aGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCcgcgcg                                                                                   >  1:312363/1‑140 (MQ=255)

                                                      aaTTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGAt                                                                              >  2:583586/1‑140 (MQ=255)

                                                        ttttCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATgg                                                                            <  1:549046/140‑1 (MQ=255)

                                                             cccGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCAt                                                                       <  1:344791/140‑1 (MQ=255)

                                                             cccGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCAt                                                                       <  2:275308/140‑1 (MQ=255)

                                                                        cAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACa                                                            <  2:649883/140‑1 (MQ=255)

                                                                               tGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTc                                                     >  2:536920/1‑140 (MQ=255)

                                                                               tGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTc                                                     >  2:208800/1‑140 (MQ=255)

                                                                               tGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTc                                                     >  1:42893/1‑140 (MQ=255)

                                                                                  gTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGcc                                                  <  1:174970/140‑1 (MQ=255)

                                                                                  gTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGcc                                                  <  1:208800/140‑1 (MQ=255)

                                                                                        cccAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGttt                                            <  1:36714/140‑1 (MQ=255)

                                                                                            gCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACg                                        >  2:454443/1‑140 (MQ=255)

                                                                                                       aTGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGttt                             >  2:250394/1‑140 (MQ=255)

                                                                                                       aTGGACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGttt                             >  1:573904/1‑140 (MQ=255)

                                                                                                          gACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCAc                          >  2:330308/1‑140 (MQ=255)

                                                                                                          gACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCAc                          <  2:42893/140‑1 (MQ=255)

                                                                                                          gACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCAc                          >  1:344349/1‑140 (MQ=255)

                                                                                                          gACTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCAc                          >  1:290696/1‑140 (MQ=255)

                                                                                                            cTCGTTTTCACCAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCt                        >  2:353267/1‑140 (MQ=255)

                                                                                                                       cAAGCAGTTTGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCTGGTAGCGGTCg             <  2:312363/140‑1 (MQ=255)

                                                                                                                              tttGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCTGGTAGCGGTCGCCCGCGt      >  2:548974/1‑140 (MQ=255)

                                                                                                                                tGATGTCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCTGGTAGCGGTCGCCCGCGTCg    >  2:727003/1‑140 (MQ=255)

                                                                                                                                  atatCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCTGGTAGCGGTCGCCCGCGTCGAt  <  1:830346/140‑1 (MQ=255)

CACATCATCCTCTTCGAGATAAGAGCCGGAGCGCACTTCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATATCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCTGGTAGCGGTCGCCCGCGTCGAT  >  NC_000913/2123117‑2123386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A