Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,445,459	G→A	G34G (GGC→GGT)	yfcA ←	conserved inner membrane protein YfcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,445,459	0	G	A	100.0%	131.4 / NA	44	G34G (GGC→GGT)	yfcA	conserved inner membrane protein YfcA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/26); total (18/26)

AAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGG  >  NC_000913/2445322‑2445594

aaaCTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACccaccacc                                                                                                                                       >  1:191080/1‑140 (MQ=255)

aaaCTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACccaccacc                                                                                                                                       <  1:564311/140‑1 (MQ=255)

        cACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCTAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCaa                                                                                                                               >  1:859130/1‑140 (MQ=255)

        cACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCaa                                                                                                                               >  2:148159/1‑140 (MQ=255)

                     aTAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCCGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGTGTAACCCACCACCACCGGCCATCGCGGCGATaaa                                                                                                                  >  2:863649/1‑140 (MQ=255)

                     aTAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATaaa                                                                                                                  >  2:674603/1‑140 (MQ=255)

                     aTAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATaaa                                                                                                                  <  2:565052/140‑1 (MQ=255)

                                agtagCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCAAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCa                                                                                                       >  2:613988/1‑140 (MQ=255)

                                 gtagCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCAt                                                                                                      <  1:649189/140‑1 (MQ=255)

                                  tagCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATg                                                                                                     >  1:222969/1‑140 (MQ=255)

                                    gCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGc                                                                                                   <  1:210275/140‑1 (MQ=255)

                                     cGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCg                                                                                                  <  2:858300/140‑1 (MQ=255)

                                       gAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATgg                                                                                                    >  2:180183/1‑136 (MQ=255)

                                       gAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATgg                                                                                                    <  1:180183/136‑1 (MQ=255)

                                                  gccgcAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGaa                                                                                     <  1:148159/140‑1 (MQ=255)

                                                  gccgcAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGaa                                                                                     >  2:587550/1‑140 (MQ=255)

                                                  gccgcAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGaa                                                                                     <  1:587550/140‑1 (MQ=255)

                                                   ccgcAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAAc                                                                                    >  1:666276/1‑140 (MQ=255)

                                                             gCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAAc                                                                          >  1:190710/1‑140 (MQ=255)

                                                              cAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCAGGGCGACAAAAAAGAGAACTCCCACCAACa                                                                         >  2:546821/1‑140 (MQ=255)

                                                               aGTTTATTGGTTGCTAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAg                                                                        <  2:859130/140‑1 (MQ=255)

                                                                gTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGc                                                                       <  1:687261/140‑1 (MQ=255)

                                                                    tttGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCgggg                                                                   <  2:445502/139‑1 (MQ=255)

                                                                    tttGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGGGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCgggg                                                                   <  2:436645/139‑1 (MQ=255)

                                                                    aTTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCCCCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCgggg                                                                   <  1:780409/140‑1 (MQ=255)

                                                                    aTTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCgggg                                                                   <  1:611361/140‑1 (MQ=255)

                                                                    aTTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCgggg                                                                   <  2:834398/140‑1 (MQ=255)

                                                                    aTTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCgggg                                                                   <  2:641155/140‑1 (MQ=255)

                                                                    aTTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCgggg                                                                   <  2:222969/140‑1 (MQ=255)

                                                                        gTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAAc                                                               >  2:759732/1‑140 (MQ=255)

                                                                           gCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCAt                                                            >  1:865111/1‑140 (MQ=255)

                                                                              aGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATaaa                                                         <  1:834151/140‑1 (MQ=255)

                                                                               gcgcATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAAc                                                        <  1:613988/140‑1 (MQ=255)

                                                                                cgcATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACa                                                       >  2:465277/1‑140 (MQ=255)

                                                                                 gcATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAg                                                      <  2:666276/140‑1 (MQ=255)

                                                                                         gggAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTaa                                              <  1:674603/140‑1 (MQ=255)

                                                                                           gagaCATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAAc                                            <  2:190710/140‑1 (MQ=255)

                                                                                                 tCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTcc                                      >  2:682628/1‑140 (MQ=255)

                                                                                                       cTGCCATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGa                                >  2:642613/1‑140 (MQ=255)

                                                                                                          ccATCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCa                             >  2:489683/1‑140 (MQ=255)

                                                                                                             tCAATGCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGt                          <  1:147939/140‑1 (MQ=255)

                                                                                                                  gCCGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCa                     <  1:682628/140‑1 (MQ=255)

                                                                                                                    cGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATc                   >  1:540307/1‑140 (MQ=255)

                                                                                                                    cGGAATGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATc                   >  1:585175/1‑140 (MQ=255)

                                                                                                                       aaTGGTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAg                <  1:542516/140‑1 (MQ=255)

                                                                                                                           gTGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAgc            >  2:551540/1‑140 (MQ=255)

                                                                                                                            tGAGTAACCCACCACCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAgcg           <  2:540307/140‑1 (MQ=255)

                                                                                                                            tGAGTAACCCACCACCACCGGCAATCGAGTCGATAAAGCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAgcg           <  2:644609/140‑1 (MQ=255)

                                                                                                                              aGTAACCCACCCCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGcc         <  1:489683/140‑1 (MQ=255)

                                                                                                                                     caccaccaccgGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAgcgc          <  1:434209/132‑1 (MQ=255)

                                                                                                                                     caccaccaccgGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAgcgc          >  2:434209/1‑132 (MQ=255)

                                                                                                                                     caccaccaccgGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAgg  >  2:368030/1‑140 (MQ=255)

AAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGG  >  NC_000913/2445322‑2445594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A