Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,594,791	T→C	T357A (ACG→GCG)	tmcA ←	tRNA(Met) cytidine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,594,791	0	T	C	100.0%	89.3 / NA	27	T357A (ACG→GCG)	tmcA	tRNA(Met) cytidine acetyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/14); total (13/14)

TGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGTCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATGGGTGAAGTTTTCATCGTCAAAAACCAGTGCCTCGCTGACCATTTTTTCCAGCGGGCATCCCTGCGCCCAGCGGATCGGCTGTTGCAGTTC  >  NC_000913/2594667‑2594923

tGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGCTAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGa                                                                                                                       >  2:682028/1‑140 (MQ=255)

tGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGa                                                                                                                       <  1:891220/140‑1 (MQ=255)

     aaaaTGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGa                                                                                                                  <  2:364528/140‑1 (MQ=255)

          gTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAata                                                                                                             <  1:634068/140‑1 (MQ=255)

          gTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAata                                                                                                             <  2:39502/140‑1 (MQ=255)

            tGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATAtt                                                                                                           <  1:850348/140‑1 (MQ=255)

               ccTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGcc                                                                                                        <  2:830533/140‑1 (MQ=255)

                  ggTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTtg                                                                                                     >  1:561459/1‑140 (MQ=255)

                   gTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATg                                                                                                                   >  1:545274/1‑125 (MQ=255)

                   gTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATg                                                                                                                   <  2:545274/125‑1 (MQ=255)

                        tccatcATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGgtgt                                                                                               <  1:121075/140‑1 (MQ=255)

                             catcCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATggg                                                                                          >  1:239741/1‑140 (MQ=255)

                             catcCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATggg                                                                                          >  2:566774/1‑140 (MQ=255)

                                 cGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATGGGTGaa                                                                                      >  2:6205/1‑140 (MQ=255)

                                  ggCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTACCTTGTGGTGTATGGGTGAAg                                                                                     >  2:459662/1‑140 (MQ=255)

                                   gCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGTGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGTGGAAATGACAATATTGCCTTGTGGTGTATGGGTGAAGt                                                                                    >  2:536165/1‑140 (MQ=255)

                                    cGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATGGGTGAAGtt                                                                                   >  2:218241/1‑140 (MQ=255)

                                        aaTCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATGGGTGAAGTTTTCa                                                                               <  2:239741/140‑1 (MQ=255)

                                         aTCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATGGGTGAAGTTTTCAt                                                                              <  2:122150/140‑1 (MQ=255)

                                                                         aCAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATGGGTGAAGTTTTCATCGTCAAAAACCAGTGCCTCGCTGACCAttttt                                              >  1:661719/1‑140 (MQ=255)

                                                                             gagCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATGGGTGAAGTTTTCATCGTCAAAAACCAGTGCCTCGCTGACCATTTTTTCCa                                          <  1:566774/140‑1 (MQ=255)

                                                                                cTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATGGGTGAAGTTTTCATCGTCAAAAACCAGTGCCTCGCTGACCATTTTTTCCAGCg                                       >  2:341702/1‑140 (MQ=255)

                                                                                   aTAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATGGGTGAAGTTTTCATCGTCAAAAACCAGTGCCTCGCTGACCATTTTTTCCAGCGGGc                                    >  2:130404/1‑140 (MQ=255)

                                                                                       aCCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATGGGTGAAGTTTTCATCGTCAAAAACCAGTGCCTCGCTGACCATTTTTTCCAGCGGGCATcc                                <  1:536165/140‑1 (MQ=255)

                                                                                               cGGCGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATGGGTGAAGTTTTCATCGTCAAAAACCAGTGCCTCGCTGACCATTTTTTCCAGCGGGCATCCCTGCGccc                        <  2:468261/140‑1 (MQ=255)

                                                                                                  cGTTTCTGGATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATGGGTGAAGTTTTCATCGTCAAAAACCAGTGCCTCGCTGACCATTTTTTCCAGCGGGCATCCCTGCGCCCAGc                     >  2:633166/1‑140 (MQ=255)

                                                                                                          gATCGCTTTGCCATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATGGGTGAAGTTTTCATCGTCAAAAACCAGTGCCTCGCTGACCATTTTTTCCAGCGGGCATCCCTGCGCCCAGCGGATCGGc             <  2:661719/140‑1 (MQ=255)

                                                                                                               cTTTGCCGTAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTAGTGGTGTATGGGTGAAGTTTTCATCGTCAAAAACCAGTGCCTCGCTGACCATTTTTTCCAGCGGGCATCCCTGCGCCCAGCGGATCGGCTGTTg        >  1:777277/1‑140 (MQ=255)

                                                                                                                     cATAACGCCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATGGGTGAAGTTTTCATCGTCAAAAACCAGTGCCTCGCTGACCATTTTTTCCAGCGGGCATCCCTGCGCCCAGCGGATCGGCTGTTGCAGTTc  >  2:124062/1‑140 (MQ=255)

TGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCGAAGTCCGATAGTGCGCACCAGACAAGAGCTGATAAACCTTTAACGGCGTTTCTGGATCGCTTTGCCATAACGTCTGTTCAAATGCGGAAATGACAATATTGCCTTGTGGTGTATGGGTGAAGTTTTCATCGTCAAAAACCAGTGCCTCGCTGACCATTTTTTCCAGCGGGCATCCCTGCGCCCAGCGGATCGGCTGTTGCAGTTC  >  NC_000913/2594667‑2594923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A