Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,604,940	T→C	S44P (TCA→CCA)	hyfD →	hydrogenase 4 component D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,604,940	0	T	C	100.0%	163.2 / NA	46	S44P (TCA→CCA)	hyfD	hydrogenase 4 component D
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/27); total (19/27)

ACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGG  >  NC_000913/2604804‑2605076

aCGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCAc                                                                                                                                       >  2:725219/1‑140 (MQ=255)

aCGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCAc                                                                                                                                       >  2:497909/1‑140 (MQ=255)

   gAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGa                                                                                                                                    >  1:587513/1‑140 (MQ=255)

           aaaaTCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCcgcg                                                                                                                            >  1:17189/1‑140 (MQ=255)

           aaaaTCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCcgcg                                                                                                                            <  1:650426/140‑1 (MQ=255)

            aaaTCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGt                                                                                                                           >  2:846381/1‑140 (MQ=255)

              aTCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGttt                                                                                                                         >  2:317333/1‑140 (MQ=255)

              aTCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGttt                                                                                                                         >  2:32462/1‑140 (MQ=255)

                cTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTa                                                                                                                       >  2:382738/1‑140 (MQ=255)

                 ttGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTAt                                                                                                                      >  1:608154/1‑140 (MQ=255)

                              gTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAg                                                                                                         <  1:151139/140‑1 (MQ=255)

                               ttATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGt                                                                                                        <  1:317333/140‑1 (MQ=255)

                                     ctgcCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGt                                                                                                  <  1:382738/140‑1 (MQ=255)

                                       gcCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGTGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCa                                                                                                >  1:625841/1‑140 (MQ=255)

                                                cGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCacgttgacgt                                                                                       <  1:32462/140‑1 (MQ=255)

                                                  gcgcACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCacgttgacgttg                                                                                     >  2:27494/1‑140 (MQ=255)

                                                  gcgcACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCacgttgacgttg                                                                                     >  2:703194/1‑140 (MQ=255)

                                                     cACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTc                                                                                  <  2:420728/140‑1 (MQ=255)

                                                     cACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTc                                                                                  <  1:770198/140‑1 (MQ=255)

                                                             gtttcgtttTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTggg                                                                          >  1:788330/1‑140 (MQ=255)

                                                               ttcgtttTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTggggg                                                                        <  1:282327/140‑1 (MQ=255)

                                                               ttcgtttTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTggggg                                                                        >  1:706239/1‑140 (MQ=255)

                                                                 cgtttTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGAt                                                                      <  1:770206/140‑1 (MQ=255)

                                                                  gtttTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGAtg                                                                     >  2:320482/1‑140 (MQ=255)

                                                                     ttCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTgg                                                                  <  1:317832/140‑1 (MQ=255)

                                                                      tCGCCACAACGTCGGGCCGCCGAATGTGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGc                                                                 <  2:625841/140‑1 (MQ=255)

                                                                      tCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGc                                                                 <  2:569901/140‑1 (MQ=255)

                                                                       cGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCg                                                                <  1:32659/140‑1 (MQ=255)

                                                                            caACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCgttgtt                                                           <  1:47615/140‑1 (MQ=255)

                                                                                gTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGc                                                       <  1:725219/140‑1 (MQ=255)

                                                                                gTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGc                                                       <  2:184309/140‑1 (MQ=255)

                                                                                 tCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGGTCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGcc                                                      <  1:565850/140‑1 (MQ=255)

                                                                                 tCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGcc                                                      <  1:497909/140‑1 (MQ=255)

                                                                                 tCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGCCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGcc                                                      <  2:68392/140‑1 (MQ=255)

                                                                                     gccgccGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGt                                                  <  1:453466/140‑1 (MQ=255)

                                                                                       cgccgAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAGCGTGGGGGATGTGGCGTTGTTTGGCCTGGTCa                                                <  2:25545/140‑1 (MQ=255)

                                                                                       cgccgAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCa                                                <  1:27494/140‑1 (MQ=255)

                                                                                         ccgAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCAtt                                              >  1:357781/1‑140 (MQ=255)

                                                                                                        tGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACg                               <  2:17189/140‑1 (MQ=255)

                                                                                                        tGTTCGCCGCGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACg                               <  2:706239/140‑1 (MQ=255)

                                                                                                              ccgcGCTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGAtt                         <  2:608154/140‑1 (MQ=255)

                                                                                                                   cTGACCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGtt                    <  2:357781/140‑1 (MQ=255)

                                                                                                                      aCCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGt                                  <  2:672030/123‑1 (MQ=255)

                                                                                                                      aCCACGCTGTGCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGt                                  >  1:672030/1‑123 (MQ=255)

                                                                                                                               tgCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGtt        >  2:750880/1‑140 (MQ=255)

                                                                                                                                gCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGtt                    >  1:873202/1‑127 (MQ=255)

                                                                                                                                gCATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGtt                    <  2:873202/127‑1 (MQ=255)

                                                                                                                                 cATGTTGCCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTtc      <  1:591598/140‑1 (MQ=255)

                                                                                                                                     ttgcCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTTGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCgg  >  2:486865/1‑140 (MQ=255)

ACGGAATATGGAAAATCTTGCTCTGACGACGTTATTGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGCCGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCACTGATCTCCGCGTTTTATCAGGCCGATAAAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCGTTGTTTGGCCTGGTCATTGATCGCGTGAGTACGCTGATTCTGTTTGTGGTGGTGTTTCTCGG  >  NC_000913/2604804‑2605076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A