Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R1 163 52.9 1786516 99.3% 1774010 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,910,211 A→G W159R (TGG→CGG)  mazG ← nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,910,2110AG90.2% 144.5 / 5.4 51W159R (TGG→CGG) mazGnucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base G (25/21);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACG  >  NC_000913/2910074‑2910346
                                                                                                                                         |                                                                                                                                       
cAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGAt                                                                                                                                       >  2:463266/1‑140 (MQ=255)
            ggCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGt                                                                                                                           <  1:550624/140‑1 (MQ=255)
             gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGtt                                                                                                                          <  1:157698/140‑1 (MQ=255)
             gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGtt                                                                                                                          >  2:680639/1‑140 (MQ=255)
                     cagGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAAc                                                                                                                  >  2:218860/1‑140 (MQ=255)
                      agGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACg                                                                                                                 <  2:47455/140‑1 (MQ=255)
                       ggTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGt                                                                                                                >  1:79177/1‑140 (MQ=255)
                                tttCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGAtt                                                                                                       >  1:469081/1‑140 (MQ=255)
                                   cctcctccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGAttttt                                                                                                    <  1:136254/140‑1 (MQ=255)
                                     tcctccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTg                                                                                                  >  1:714493/1‑140 (MQ=255)
                                     tcctccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTg                                                                                                  >  1:61296/1‑140 (MQ=255)
                                      cctccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTgc                                                                                                 <  1:417088/140‑1 (MQ=255)
                                         ccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGcgca                                                                                              <  2:714493/140‑1 (MQ=255)
                                         ccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGcgca                                                                                              <  1:486741/140‑1 (MQ=255)
                                            gTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGcgcacgc                                                                                           <  2:298059/140‑1 (MQ=255)
                                             tttAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGcgcacgca                                                                                          >  2:570035/1‑140 (MQ=255)
                                             tttAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGcgcacgc                                                                                           <  2:649505/139‑1 (MQ=255)
                                             tttAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGcgcacgc                                                                                           >  1:649505/1‑139 (MQ=255)
                                                 gCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTaa                                                                                      <  1:195081/140‑1 (MQ=255)
                                                 gCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTaa                                                                                      <  2:806748/140‑1 (MQ=255)
                                                      gTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCg                                                                                 >  2:313633/1‑140 (MQ=255)
                                                      gTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCg                                                                                 <  1:34460/140‑1 (MQ=255)
                                                          acaacaGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTaa                                                                             >  2:67658/1‑140 (MQ=255)
                                                             acaGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACt                                                                          <  1:171146/140‑1 (MQ=255)
                                                                  cTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAg                                                                     <  1:570035/140‑1 (MQ=255)
                                                                  cTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAg                                                                     <  2:61296/140‑1 (MQ=255)
                                                                    gCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGa                                                                   <  1:313633/140‑1 (MQ=255)
                                                                       gcgcTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAata                                                                <  2:79177/140‑1 (MQ=255)
                                                                         gcTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATc                                                              >  2:137134/1‑140 (MQ=255)
                                                                            tCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTc                                                           <  2:469081/140‑1 (MQ=255)
                                                                                    cACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCg                                                   >  2:671182/1‑140 (MQ=255)
                                                                                       cTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAata                                                                >  2:431375/1‑124 (MQ=255)
                                                                                       cTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAata                                                                <  1:431375/124‑1 (MQ=255)
                                                                                       cTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAAt                                                <  2:499855/140‑1 (MQ=255)
                                                                                       cTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAAt                                                >  1:514452/1‑140 (MQ=255)
                                                                                        tcgtcgATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAAtg                                               <  2:341427/140‑1 (MQ=255)
                                                                                          gtcgATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAAtgct                                             >  2:242080/1‑140 (MQ=255)
                                                                                          gtcgATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAAtgct                                             >  2:405511/1‑140 (MQ=255)
                                                                                            cgATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACACATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAAtgctgc                                           >  1:846757/1‑140 (MQ=255)
                                                                                              aTCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTgcgc                                         >  1:314650/1‑140 (MQ=255)
                                                                                                     cGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTgc                                  >  2:240085/1‑140 (MQ=255)
                                                                                                        aGACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTgcgcg                               <  2:485910/140‑1 (MQ=255)
                                                                                                         gACTTTATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTgcgcgc                              <  1:284187/140‑1 (MQ=255)
                                                                                                             ttATCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGctc                          >  1:51943/1‑140 (MQ=255)
                                                                                                               aTCGACTACCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCtt                        >  2:508519/1‑140 (MQ=255)
                                                                                                                      aCCGGACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGA‑GAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTg                >  2:444342/1‑140 (MQ=255)
                                                                                                                          gACCAAGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGAtt             >  1:751484/1‑140 (MQ=255)
                                                                                                                            ccAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTg           <  2:514452/140‑1 (MQ=255)
                                                                                                                               aGCGTCGTCCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTc        >  2:525209/1‑140 (MQ=255)
                                                                                                                                 tgtcgtcCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTccc      >  1:5161/2‑140 (MQ=255)
                                                                                                                                   tcgtcCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCaa    <  1:525209/140‑1 (MQ=255)
                                                                                                                                     gtcCGATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACg  >  2:136526/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAATGCTGCGCTTTCTGCGCGCGCTCTTCGGTTTTGATTTGCTCCCAACG  >  NC_000913/2910074‑2910346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A