Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,008,407	+G	coding (898/1197 nt)	ygeX →	2,3‑diaminopropionate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,008,404	1	.	G	100.0%	161.1 / NA	46	W299G (TGG→GGG)	ygeX	2,3‑diaminopropionate ammonia‑lyase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (27/19); total (27/19)

CACAGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCT‑GGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGG  >  NC_000913/3008269‑3008528

cacaGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGctgggct‑gggg                                                                                                                          <  2:733062/140‑2 (MQ=255)

   aGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGctgggct‑ggggaaa                                                                                                                       <  1:290109/140‑5 (MQ=255)

             ttgAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTaa                                                                                                              >  1:640151/1‑140 (MQ=255)

               gAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACt                                                                                                            >  2:22130/1‑140 (MQ=255)

               gAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACt                                                                                                            >  1:691783/1‑140 (MQ=255)

               gAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACt                                                                                                            >  1:362544/1‑140 (MQ=255)

               gAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACt                                                                                                            >  1:491646/1‑140 (MQ=255)

               gAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACt                                                                                                            >  1:51896/1‑140 (MQ=255)

                    tGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGcc                                                                                                       >  1:654208/1‑140 (MQ=255)

                     gACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCa                                                                                                      <  2:691783/140‑1 (MQ=255)

                     gACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCa                                                                                                      <  1:222507/140‑1 (MQ=255)

                     gACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAAGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCa                                                                                                      >  1:444343/1‑140 (MQ=255)

                       cAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCAcc                                                                                                    <  1:277242/140‑1 (MQ=255)

                               aCTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCAt                                                                                            >  2:596461/1‑140 (MQ=255)

                               aCTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCAt                                                                                            <  1:106066/140‑1 (MQ=255)

                                    aTTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCAtct                                                                                          <  1:492043/137‑1 (MQ=255)

                                    aTTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCAtct                                                                                          >  2:492043/1‑137 (MQ=255)

                                             tCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACa                                                                              >  2:115970/1‑140 (MQ=255)

                                             tCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACa                                                                              >  1:879077/1‑140 (MQ=255)

                                                   gTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTg                                                                        <  1:115970/140‑1 (MQ=255)

                                                    tCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGc                                                                       <  1:139497/140‑1 (MQ=255)

                                                          gCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCAtt                                                                 <  1:596461/140‑1 (MQ=255)

                                                           cGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTa                                                                >  1:831249/1‑140 (MQ=255)

                                                            gACATCGTTAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCATTGCCGCATTAg                                                               >  1:762813/1‑140 (MQ=255)

                                                              cATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGt                                                             >  2:35739/1‑140 (MQ=255)

                                                                  gTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATgc                                                         <  1:776338/140‑1 (MQ=255)

                                                                  gTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATgc                                                         <  2:654208/140‑1 (MQ=255)

                                                                           ggCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTgg                                                >  2:425366/1‑140 (MQ=255)

                                                                           ggCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCACATTAGGTATGCGCGTGCTgg                                                <  2:879077/140‑1 (MQ=255)

                                                                             cGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGt                                              <  2:665529/140‑1 (MQ=255)

                                                                              ggTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTa                                             <  2:362544/140‑1 (MQ=255)

                                                                                   tatGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCGATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCg                                        <  2:51896/140‑1 (MQ=255)

                                                                                            catcatGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAAc                               >  1:147592/1‑140 (MQ=255)

                                                                                                atGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGAcc                           <  1:402/140‑1 (MQ=255)

                                                                                                      ggcctggcctGTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGca                     >  1:71847/1‑140 (MQ=255)

                                                                                                       gcctggcctgTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTa                                      >  2:241454/1‑122 (MQ=255)

                                                                                                       gcctggcctgTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTa                                      <  1:241454/122‑1 (MQ=255)

                                                                                                        cctggcctgTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGcatc                   >  1:598706/1‑140 (MQ=255)

                                                                                                            gcctgTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAAc                               <  2:768134/124‑1 (MQ=255)

                                                                                                            gcctgTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAAc                               >  1:768134/1‑124 (MQ=255)

                                                                                                            gcctgTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCAtct               >  2:176982/1‑140 (MQ=255)

                                                                                                            gcctgTGGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCACGCATCAtct               >  1:359521/1‑140 (MQ=255)

                                                                                                                 tgGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGt          >  2:127618/1‑140 (MQ=255)

                                                                                                                 tgGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGt          >  1:296228/1‑140 (MQ=255)

                                                                                                                 tgGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGt          >  2:99017/1‑140 (MQ=255)

                                                                                                                 tgGCGAACCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTAC‑GCAACGACCCGCGCATCATCTCCGGTg         >  1:519892/1‑140 (MQ=255)

                                                                                                                       aCCTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTAC‑GCAACGACCCGCGCATCATCTCCGGTGAATCCg   <  2:519892/140‑1 (MQ=255)

                                                                                                                         cTAACCCGCTGGGCTGGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCgg  <  2:598706/140‑1 (MQ=255)

CACAGCATTATTGTTGAACCTGACAAAGCTGACTGTATTTATCGCTCCGGCGTCAAAGGCGACATCGTCAACGTTGGCGGTGATATGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCT‑GGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAATCCGTACGGCAACGACCCGCGCATCATCTCCGGTGAATCCGG  >  NC_000913/3008269‑3008528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A