Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	163	52.9	1786516	99.3%	1774010	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,300,739	A→G	I7T (ATT→ACT)	yhbS ←	putative acyltransferase with acyl‑CoA N‑acyltransferase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,300,739	0	A	G	100.0%	119.3 / NA	34	I7T (ATT→ACT)	yhbS	putative acyltransferase with acyl‑CoA N‑acyltransferase domain
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/11); total (23/11)

ACCTTCGTCATCTGTCGCCACCAGCCCCAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTT  >  NC_000913/3300606‑3300870

acctTCGTCATCTGTCGCCACCAGCCCCAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGATTCTa                                                                                                                               >  1:800501/1‑140 (MQ=255)

     cGTCATCTGTCGCCACCAGCCCCAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGa                                                                                                                          >  2:33882/1‑140 (MQ=255)

     cGTCATCTGTCGCCACCAGCCCCAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGa                                                                                                                          >  1:32076/1‑140 (MQ=255)

         aTCTGTCGCCACCAGCCCCAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTa                                                                                                                      <  1:792220/140‑1 (MQ=255)

             gTCGCCACCAGCCCCAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCAt                                                                                                                  <  1:278865/140‑1 (MQ=255)

             gTCGCCACCAGCCCCAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCAt                                                                                                                  <  2:30711/140‑1 (MQ=255)

               cGCCACCAGCCCCAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATgg                                                                                                                >  2:888960/1‑140 (MQ=255)

                gCCACCAGCCCCAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGt                                                                                                               <  1:296238/140‑1 (MQ=255)

                gCCACCAGCCCCAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGt                                                                                                               <  1:515015/140‑1 (MQ=255)

                  caccaGCCCCAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTc                                                                                                             <  1:33882/140‑1 (MQ=255)

                     caGCCCCAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCAcc                                                                                                          >  1:475852/1‑140 (MQ=255)

                         cccAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTAcc                                                                                                      <  2:214164/140‑1 (MQ=255)

                            aGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGAt                                                                                                   <  1:452448/140‑1 (MQ=255)

                            aGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGAt                                                                                                   <  2:15684/140‑1 (MQ=255)

                                                 cGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGcgcg                                                                              <  1:255274/140‑1 (MQ=255)

                                                  gCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCgg                                                                             >  1:892693/1‑140 (MQ=255)

                                                      aTCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGtttt                                                                         >  1:530545/1‑140 (MQ=255)

                                                      aTCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGtttt                                                                         >  1:509937/1‑140 (MQ=255)

                                                          tGACCCAGCTCCGCTCCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATCCCCGGCGCATCAATGGGAGTTTCTCCTCGAATTAGCATGGTTCCCCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCAtt                                                                     <  2:628688/140‑1 (MQ=255)

                                                            acccAGCTCCGCTCCCGCATCCCTTTCGAATGAGCGACGCAGCAGGGCATCAATCCCCGGCGCATCAAGGGGAGTTTCTACTCGAATTAGCATGGTTCCCCTCCCGATTTCTGTTTGTTTTCAGGCGCGGTTTTCATccc                                                                   <  2:289749/138‑1 (MQ=255)

                                                                    tcgcttcCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTcaa                                                           >  1:70631/1‑140 (MQ=255)

                                                                    tcgcttcCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTcaa                                                           >  1:389025/1‑140 (MQ=255)

                                                                            gCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATcc                                                   >  2:450214/1‑140 (MQ=255)

                                                                                  ttttCGAATGAGCGACGCAGCAGGGCATCAATCCCCGGCGCATCAAGGGGAGTTTCTACTCGAATTAGCATGGTTCCCCTACCGATGTCTGTTTGGTTTCAGGCGCGTTTTTCATTCCCGCCTCAACAAAATCCGCCAGt                                             <  2:183966/139‑1 (MQ=255)

                                                                                  ttttCAAATGAGCAACCCAGAGGGCCATCAATCCCCGGCCCATCAATGGGATTTTCTCCCCAAATTAGCATGTTTCACCTACCGATGTTTTTTTGGTTCCAGGCGCGGTTTTCATCCCCGCCTCAACAAAATCCGCCAGt                                             <  2:32076/139‑1 (MQ=255)

                                                                                  cTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGt                                             >  1:687752/1‑140 (MQ=255)

                                                                                  cTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGt                                             >  1:299210/1‑140 (MQ=255)

                                                                                     tCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTgc                                          >  2:364234/1‑140 (MQ=255)

                                                                                           gggCGACGCAGCAGGCCACCAACACCCGGCGAATAAATGGGATTTTCTACCCGAATAAGCATGTTTCCCCTACCGATTTCTGTTTGGTTTCAGGCGCGTTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTgcagcagc                                    <  2:514619/138‑1 (MQ=255)

                                                                                                        gggCATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTaact                       >  1:514619/1‑138 (MQ=255)

                                                                                                           cATCAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCtt                    >  1:837050/1‑140 (MQ=255)

                                                                                                            aTCAATCCCCGGCGCATCAAGGGGATTTTCTACTCGATTTAGTATGGTTCCCCTACCGTTGTTTTTTTGTTTTCAGGCGCGTTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCttt                   <  2:837050/140‑1 (MQ=255)

                                                                                                              cAATACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCg                 >  1:527933/1‑140 (MQ=255)

                                                                                                                aTACCCGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGc               >  1:15672/1‑140 (MQ=255)

                                                                                                                     cGGCGCATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTg          >  2:311662/1‑140 (MQ=255)

                                                                                                                        cgcATCAATGGGAGTTTCTACTCGAATTAGCATGGTTGACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATGATGCGTAAGGCTTTCGGCATGTGCTc       >  2:789936/1‑140 (MQ=255)

                                                                                                                        cgcATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTc       <  2:509937/140‑1 (MQ=255)

                                                                                                                        cgcATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTc       >  2:33122/1‑140 (MQ=255)

                                                                                                                        cgcATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTc       <  2:530545/140‑1 (MQ=255)

                                                                                                                        cgcATCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTc       >  2:853047/1‑140 (MQ=255)

                                                                                                                        cgcATAAATGGGATTTTCTACTCGATTTAGCATGTTTCCCCTACCGTTTTCTTTTTGGTTTCGGGCGGGTTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTc       <  2:15672/140‑1 (MQ=255)

                                                                                                                            tCAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGt   <  2:687752/140‑1 (MQ=255)

                                                                                                                             cAATGGGAGTTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGtt  >  2:235035/1‑140 (MQ=255)

ACCTTCGTCATCTGTCGCCACCAGCCCCAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAATTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTTCAGGCGCGGTTTTCATTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTT  >  NC_000913/3300606‑3300870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A