Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R1 163 52.9 1786516 99.3% 1774010 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,541,335 A→G T127A (ACT→GCT)  fimB → regulator for fimA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,541,3350AG97.7% 298.1 / ‑3.8 87T127A (ACT→GCT) fimBregulator for fimA
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (41/44);  total (42/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATT  >  NC_000913/4541201‑4541466
                                                                                                                                      |                                                                                                                                   
tGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTcc                                                                                                                                <  1:488731/140‑1 (MQ=255)
tGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTcc                                                                                                                                <  1:40237/140‑1 (MQ=255)
    tAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCggtg                                                                                                                            >  1:111693/1‑140 (MQ=255)
    tAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCggtg                                                                                                                            <  1:810999/140‑1 (MQ=255)
    tAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCggtg                                                                                                                            >  2:586060/1‑140 (MQ=255)
       agaagTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTa                                                                                                                         >  1:723855/1‑140 (MQ=255)
       agaagTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTa                                                                                                                         >  1:513777/1‑140 (MQ=255)
         aagTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAAt                                                                                                                       >  1:636565/1‑140 (MQ=255)
              cAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCgg                                                                                                                  <  1:503828/140‑1 (MQ=255)
               aGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCggg                                                                                                                 <  1:447158/140‑1 (MQ=255)
                ggCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGt                                                                                                                <  1:235139/140‑1 (MQ=255)
                 gCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGtt                                                                                                               <  2:834765/140‑1 (MQ=255)
                  cTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTg                                                                                                              <  2:293413/140‑1 (MQ=255)
                   tttAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGt                                                                                                             <  2:677458/140‑1 (MQ=255)
                   tttAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGt                                                                                                             >  2:39926/1‑140 (MQ=255)
                   tttAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAACCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGt                                                                                                             <  2:84468/140‑1 (MQ=255)
                            cTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGgaga                                                                                                    >  1:489000/1‑140 (MQ=255)
                            cTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGgaga                                                                                                    <  2:43995/140‑1 (MQ=255)
                                     tATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGc                                                                                           <  1:34268/140‑1 (MQ=255)
                                      aTCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGca                                                                                          <  1:39926/140‑1 (MQ=255)
                                      aTCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGca                                                                                          <  1:285204/140‑1 (MQ=255)
                                         cGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACAt                                                                                       >  2:409345/1‑140 (MQ=255)
                                             cTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTa                                                                                   >  2:471919/1‑140 (MQ=255)
                                               tCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACg                                                                                 >  1:345411/1‑140 (MQ=255)
                                                cGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGc                                                                                <  2:723855/140‑1 (MQ=255)
                                                  tACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCa                                                                              >  2:3655/1‑140 (MQ=255)
                                                  tACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCa                                                                              >  2:461672/1‑140 (MQ=255)
                                                  tACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCa                                                                              >  2:242644/1‑140 (MQ=255)
                                                  tACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCa                                                                              >  1:176601/1‑140 (MQ=255)
                                                     ccGCATGCTGAGAGCGAGCGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTc                                                                           >  2:663366/1‑140 (MQ=255)
                                                       gCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCgt                                                                         <  2:636565/140‑1 (MQ=255)
                                                        cATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCgtg                                                                        <  1:174044/140‑1 (MQ=255)
                                                          tGCTGAGAGCGAGTGGGTATTTTTATGACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGtgtg                                                                      >  1:707802/1‑140 (MQ=255)
                                                          tGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGtgtg                                                                      >  2:780457/1‑140 (MQ=255)
                                                               agagCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGtttt                                                                 >  1:172692/1‑140 (MQ=255)
                                                                gagCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTg                                                                <  1:409345/140‑1 (MQ=255)
                                                                 agCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGc                                                               >  2:421028/1‑140 (MQ=255)
                                                                 agCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGc                                                               >  2:516491/1‑140 (MQ=255)
                                                                   cGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCgt                                                                         <  2:70637/128‑1 (MQ=255)
                                                                   cGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCgt                                                                         >  1:70637/1‑128 (MQ=255)
                                                                   cGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCtt                                                             >  2:818222/1‑140 (MQ=255)
                                                                     aGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTg                                                           >  2:225274/1‑140 (MQ=255)
                                                                     aGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTg                                                           >  2:465739/1‑140 (MQ=255)
                                                                     aGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTg                                                           >  2:582763/1‑140 (MQ=255)
                                                                     aGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTg                                                           >  1:583211/1‑140 (MQ=255)
                                                                     aGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTg                                                           >  1:70118/1‑140 (MQ=255)
                                                                     aGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTg                                                           >  2:303309/1‑140 (MQ=255)
                                                                     aGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTg                                                           >  1:664431/1‑140 (MQ=255)
                                                                       tGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATGCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGc                                                         <  1:303309/140‑1 (MQ=255)
                                                                       tGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGc                                                         <  1:384042/140‑1 (MQ=255)
                                                                        gggTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCg                                                        <  2:572909/140‑1 (MQ=255)
                                                                            aTTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAata                                                    <  1:242644/140‑1 (MQ=255)
                                                                             tttttATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAatat                                                   <  2:489000/140‑1 (MQ=255)
                                                                             tttttATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAatat                                                   <  1:663366/140‑1 (MQ=255)
                                                                                  aTCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGaa                                              >  2:664795/1‑140 (MQ=255)
                                                                                   tCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAAt                                             >  1:223685/1‑140 (MQ=255)
                                                                                   tCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAAt                                             >  1:501504/1‑140 (MQ=255)
                                                                                   tCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAAt                                             >  2:119376/1‑140 (MQ=255)
                                                                                        tAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATa                                        >  1:66536/1‑140 (MQ=255)
                                                                                         aaGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATAc                                       <  2:172692/140‑1 (MQ=255)
                                                                                           ggggAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATAcgc                                     >  1:96172/1‑140 (MQ=255)
                                                                                              gAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGAc                                  <  1:471919/140‑1 (MQ=255)
                                                                                              gAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGAc                                  <  1:299104/140‑1 (MQ=255)
                                                                                              gAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGAc                                  <  2:178115/140‑1 (MQ=255)
                                                                                              gAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGAc                                  <  1:225274/140‑1 (MQ=255)
                                                                                              gAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGAc                                  <  2:66536/140‑1 (MQ=255)
                                                                                                aTCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACtt                                >  1:316671/1‑140 (MQ=255)
                                                                                                   cGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATc                             <  1:465739/140‑1 (MQ=255)
                                                                                                   cGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATc                             <  1:29379/140‑1 (MQ=255)
                                                                                                   cGCTTTCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATc                             <  1:818222/140‑1 (MQ=255)
                                                                                                      tttCTCGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAg                          >  1:254889/1‑140 (MQ=255)
                                                                                                        tctcGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGa                        <  2:70118/140‑1 (MQ=255)
                                                                                                        tctcGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGa                        <  2:223685/140‑1 (MQ=255)
                                                                                                        tctcGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGa                        <  1:3655/140‑1 (MQ=255)
                                                                                                        tctcGGCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGa                        <  2:394953/140‑1 (MQ=255)
                                                                                                             gCAACAGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATc                   >  2:862959/1‑140 (MQ=255)
                                                                                                                  aGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTggg              <  2:707802/140‑1 (MQ=255)
                                                                                                                  aGTTTTACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTggg              <  1:652189/140‑1 (MQ=255)
                                                                                                                    ttttACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCa            <  1:58538/140‑1 (MQ=255)
                                                                                                                       tACCATATTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGTATCg         <  1:193159/140‑1 (MQ=255)
                                                                                                                           atatTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAat     <  2:501504/140‑1 (MQ=255)
                                                                                                                           atatTATCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAat     <  1:582763/140‑1 (MQ=255)
                                                                                                                            tattatCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTggg              <  1:35154/130‑1 (MQ=255)
                                                                                                                            tattatCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTggg              >  2:35154/1‑130 (MQ=255)
                                                                                                                              ttatCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATAtt  >  1:31071/1‑140 (MQ=255)
                                                                                                                              ttatCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATAtt  >  2:455246/1‑140 (MQ=255)
                                                                                                                              ttatCTCGGCTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATAtt  >  2:320140/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                   
TGAATAAAGAAGTTCAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATT  >  NC_000913/4541201‑4541466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A