Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,463,046	A→G	T308A (ACC→GCC)	paaK →	phenylacetate‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,463,046	0	A	G	100.0%	252.0 / NA	69	T308A (ACC→GCC)	paaK	phenylacetate‑CoA ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/38); total (31/38)

GGGAAGATCATTTCTATCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACA  >  NC_000913/1462909‑1463182

gggAAGATCATTTCTATCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGcc                                                                                                                                        <  2:1015691/140‑1 (MQ=255)

 ggAAGATCATTTCTATCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGccc                                                                                                                                       <  1:356737/140‑1 (MQ=255)

   aaGATCATTTCTATCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGa                                                                                                                                     <  2:598775/140‑1 (MQ=255)

   aaGATCATTTCTATCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGa                                                                                                                                     <  1:573840/140‑1 (MQ=255)

    aGATCATTTCTATCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGAc                                                                                                                                    <  1:269483/140‑1 (MQ=255)

     gATCATTTCTATCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACt                                                                                                                                   <  2:963295/140‑1 (MQ=255)

     gATCATTTCTATCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACt                                                                                                                                   >  2:100310/1‑140 (MQ=255)

      aTCATTTCTATCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTg                                                                                                                                  <  1:251993/140‑1 (MQ=255)

      aTCATTTCTATCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTg                                                                                                                                  >  1:714083/1‑140 (MQ=255)

               aTCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGa                                                                                                                         <  1:967468/140‑1 (MQ=255)

                tCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGaa                                                                                                                        >  1:895809/1‑140 (MQ=255)

                tCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGaa                                                                                                                        >  2:1202768/1‑140 (MQ=255)

                tCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGaa                                                                                                                        <  1:328688/140‑1 (MQ=255)

                tCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGaa                                                                                                                        >  1:359718/1‑140 (MQ=255)

                     aaaTTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTg                                                                                                                                  >  1:221788/1‑125 (MQ=255)

                     aaaTTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTg                                                                                                                                  <  2:221788/125‑1 (MQ=255)

                     aaaTTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGgcg                                                                                                                   >  1:3972/1‑140 (MQ=255)

                     aaaTTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGgcg                                                                                                                   >  2:895879/1‑140 (MQ=255)

                         tGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGa                                                                                                               <  1:460538/140‑1 (MQ=255)

                          gTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGAc                                                                                                              >  1:286522/1‑140 (MQ=255)

                           ttAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACt                                                                                                             >  1:804953/1‑140 (MQ=255)

                               tCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATgc                                                                                                         <  2:804953/140‑1 (MQ=255)

                               tCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATgc                                                                                                         <  2:3972/140‑1 (MQ=255)

                                ccTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATgcg                                                                                                        <  2:286522/140‑1 (MQ=255)

                                ccTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATgcg                                                                                                        <  1:29435/140‑1 (MQ=255)

                                 cTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATgcgc                                                                                                       >  2:556984/1‑140 (MQ=255)

                                   cATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGcgccg                                                                                                     >  2:725809/1‑140 (MQ=255)

                                    aTGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGcgccgc                                                                                                    >  1:548092/1‑140 (MQ=255)

                                        cGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATgg                                                                                                <  1:905049/140‑1 (MQ=255)

                                         ggCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGa                                                                                               >  2:253068/1‑140 (MQ=255)

                                           cacaCCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCa                                                                                                   <  2:723730/134‑1 (MQ=255)

                                           cacaCCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCa                                                                                                   >  1:723730/1‑134 (MQ=255)

                                                cGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATc                                                                                        >  2:227591/1‑140 (MQ=255)

                                                cGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATc                                                                                        >  2:762755/1‑140 (MQ=255)

                                                cGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATc                                                                                        >  2:1059128/1‑140 (MQ=255)

                                                      ccGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGa                                                                                  <  2:714083/140‑1 (MQ=255)

                                                      ccGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGa                                                                                  <  1:762755/140‑1 (MQ=255)

                                                       cGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGAc                                                                                 <  1:556984/140‑1 (MQ=255)

                                                        gATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACg                                                                                <  1:227591/140‑1 (MQ=255)

                                                        gATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACg                                                                                <  2:264756/140‑1 (MQ=255)

                                                           ggTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAg                                                                             <  2:354526/140‑1 (MQ=255)

                                                               aaCATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGAt                                                                         <  1:100310/140‑1 (MQ=255)

                                                                     gCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATg                                                                   <  1:370231/140‑1 (MQ=255)

                                                                        aaCTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTg                                                                >  2:230483/1‑140 (MQ=255)

                                                                              tATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGattatt                                                          >  1:157444/1‑140 (MQ=255)

                                                                                ttCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTcg                                                        <  1:170296/140‑1 (MQ=255)

                                                                                  caccacGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTcgcg                                                      <  1:1104082/140‑1 (MQ=255)

                                                                                  caccacGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTcgcg                                                      <  1:895879/140‑1 (MQ=255)

                                                                                     cacGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGgtg                                                   >  1:967412/1‑140 (MQ=255)

                                                                                      acGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGgtgt                                                  <  1:1202768/140‑1 (MQ=255)

                                                                                       cGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTa                                                 >  2:903678/1‑140 (MQ=255)

                                                                                       cGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTa                                                 >  2:787752/1‑140 (MQ=255)

                                                                                       cGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTa                                                 >  2:63381/1‑140 (MQ=255)

                                                                                          tGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTcg                                                        <  2:861954/130‑1 (MQ=255)

                                                                                          tGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTcg                                                        >  1:861954/1‑130 (MQ=255)

                                                                                          tGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAAt                                              <  1:1059128/140‑1 (MQ=255)

                                                                                            aCCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGt                                            >  2:1162812/1‑140 (MQ=255)

                                                                                                      cATTGCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTca                                  >  2:1032852/1‑140 (MQ=255)

                                                                                                          gCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATTACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAAc                              <  2:343085/140‑1 (MQ=255)

                                                                                                          gCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAAc                              <  1:253068/140‑1 (MQ=255)

                                                                                                          gCCGGTCATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAAc                              <  1:1162812/140‑1 (MQ=255)

                                                                                                                cATTCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGgaag                        <  1:725809/140‑1 (MQ=255)

                                                                                                                   tCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTgg                           >  1:591030/1‑134 (MQ=255)

                                                                                                                   tCGTTACCGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTgg                           <  2:591030/134‑1 (MQ=255)

                                                                                                                         ccGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAAc                              <  1:1174293/125‑1 (MQ=255)

                                                                                                                         ccGCACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAAc                              >  2:1174293/1‑125 (MQ=255)

                                                                                                                            cACGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCa            <  2:943099/140‑1 (MQ=255)

                                                                                                                             aCGCGTGATTTAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCaa           >  2:487679/1‑140 (MQ=255)

                                                                                                                                      ttAGCCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACa  >  2:1130765/1‑140 (MQ=255)

GGGAAGATCATTTCTATCCTGAAATTGTTAATCCTCATGACGGCACACCGCTTGCCGATGGTGAACATGGCGAACTGTTATTCACCACGCTGACCAAAGAAGCATTGCCGGTCATTCGTTACCGCACGCGTGATTTAACCCGACTGTTACCAGGAACGGCGCGGACTATGCGCCGCATGGATCGCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACA  >  NC_000913/1462909‑1463182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A