Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	161	75.5	2581324	99.3%	2563254	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,781	A→G	T399A (ACC→GCC)	ydiF →	putative acetate‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,781	0	A	G	100.0%	134.6 / NA	37	T399A (ACC→GCC)	ydiF	putative acetate‑CoA transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/21); total (16/21)

CGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAAT  >  NC_000913/1776646‑1776913

cGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCgg                                                                                                                                  <  2:456342/140‑1 (MQ=255)

 gTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGt                                                                                                                                 <  2:409107/140‑1 (MQ=255)

 gTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGt                                                                                                                                 >  1:409107/1‑140 (MQ=255)

  tGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTg                                                                                                                                <  1:751340/140‑1 (MQ=255)

   gCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTgg                                                                                                                               >  1:212729/1‑140 (MQ=255)

     cATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGAt                                                                                                                             <  1:1027300/140‑1 (MQ=255)

     cATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGAt                                                                                                                             <  1:169505/140‑1 (MQ=255)

       ttCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGAttt                                                                                                                           >  1:48032/1‑140 (MQ=255)

          tGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTAtt                                                                                                                        <  2:983409/140‑1 (MQ=255)

            gATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGa                                                                                                                      >  2:243758/1‑140 (MQ=255)

                 gACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCa                                                                                                                 >  1:521684/1‑140 (MQ=255)

                     tCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGc                                                                                                             <  1:243758/140‑1 (MQ=255)

                      cccAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGcc                                                                                                            >  2:1088823/1‑140 (MQ=255)

                                          ggTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCAtttt                                                                                        <  2:212729/140‑1 (MQ=255)

                                                ggTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCgg                                                                                  <  2:48032/140‑1 (MQ=255)

                                                        tgtttgttATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCgg                                                                                  <  1:203487/132‑1 (MQ=255)

                                                        tgtttgttATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCgg                                                                                  >  2:203487/1‑132 (MQ=255)

                                                        tgtttgttATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTaa                                                                          >  1:235457/1‑140 (MQ=255)

                                                            tgttATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGc                                                                      >  2:1018033/1‑140 (MQ=255)

                                                             gttATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCg                                                                     <  1:1076921/140‑1 (MQ=255)

                                                                                          cACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACgg                                        <  1:979773/140‑1 (MQ=255)

                                                                                           aCGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGc                                       <  1:1018033/140‑1 (MQ=255)

                                                                                                    tCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGc                                       <  2:349982/131‑1 (MQ=255)

                                                                                                    tCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGc                                       >  1:349982/1‑131 (MQ=255)

                                                                                                    tCGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAat                              >  2:502529/1‑140 (MQ=255)

                                                                                                     cGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCGCATTAACTACGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAata                             <  2:504479/140‑1 (MQ=255)

                                                                                                     cGGCGTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAata                             <  1:74734/140‑1 (MQ=255)

                                                                                                         gTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGt                         >  2:1101768/1‑140 (MQ=255)

                                                                                                         gTGCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGt                         <  1:334423/140‑1 (MQ=255)

                                                                                                           gCATAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTcc                       >  2:270084/1‑140 (MQ=255)

                                                                                                             aTAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGt                         <  2:376824/136‑1 (MQ=255)

                                                                                                             aTAAATTCAATGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGt                         >  1:376824/1‑136 (MQ=255)

                                                                                                                      aTGGTAAAATCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACgg            >  2:627288/1‑140 (MQ=255)

                                                                                                                            aaaTCATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTgaag      >  1:1122822/1‑140 (MQ=255)

                                                                                                                                cATGGGCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAAt  <  2:521684/140‑1 (MQ=255)

                                                                                                                                    ggCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCaggaagga               <  1:439977/123‑1 (MQ=255)

                                                                                                                                    ggCGCCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAAt  <  1:789501/136‑1 (MQ=255)

CGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTTTTGCTGAAGTCGACCAGCACGGTAACGTCGGCGTGCATAAATTCAATGGTAAAATCATGGGCACCGGTGGATTTATTGATATCAGTGCCACTTCGAAGAAAATCATTTTCTGCGGCACATTAACTGCGGGCAGTTTAAAAACAGAAATTACCGACGGCAAATTAAATATCGTCCAGGAAGGACGGGTGAAGAAAT  >  NC_000913/1776646‑1776913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A